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transfection <molecular biology> The introduction of DNA into a recipient eukaryote cell and its subsequent integration into the recipient cells chromosomal DNA.
Usually accomplished using DNA precipitated with calcium ions though a variety of other methods can be used (e.g. Electroporation). Only about 1% of cultured cells are normally transfected.
Transfection is analogous to bacterial transformation but in eukaryotes transformation is used to describe the changes in cultured cells caused by tumour viruses.
(17 Mar 1998)
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  • Transfection - »õâ The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
    Synonyms : Transfections
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transfection Introducing DNA into eukaryotic cells, such as animal cells, is called transfection. Transfection typically involves opening transient "holes" or gates in cells to allow the entry of extracellular molecules, typically supercoiled plasmid DNA, but also siRNA, among others. Several different transfection techniques are available, including calcium phosphate transfection, lipofection, electroporation, heat shock, and proprietary transfection reagents such as Fugene. ...
Ãâó: en.wikipedia.org/wiki/Transfection
transfection The transfer of exogenous DNA into a cell.
Ãâó: www.nature.com/nrg/journal/v4/n10/glossary/nrg1180...
transfection The introduction of DNA into a cell or organism using recombinant DNA technology.
Ãâó: www.genpromag.com/Glossary~LETTER~T.html
transfection The successful virus-infection of cells following their inoculation with viral nucleic acid. (10)
Ãâó: ppathw3.cals.cornell.edu/glossary/Defs_T.htm
transfection The introduction of foreign DNA into a host cell. Source : Human Genome Project Information
Ãâó: www.genomecanada.ca/GCglossaire/glossaire/index.as...
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