| stringency | <molecular biology> Reaction conditions, notably temperature, salt, and pH that dictate the annealing of single-stranded DNA/DNA, DNA/ RNA, and RNA/RNA hybrids. at high stringency, duplexes form only between strands with perfect one-to-one complementarity, lower stringency allows annealing between strands with some degree of mismatch between bases. (16 Dec 1997) |
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| hybridisation stringency | <molecular biology> The percentage of nucleotides which must match on two unrelated single-stranded nucleic acid molecules before they will base pair with each other to form a duplex, given a certain set of physical and chemical conditions. The hybridisation stringency is used to determine when a hybridisation probe and a target nucleic acid will come together, and can be set by the researcher by varying the conditions. In general, if the percentage of matching nucleotides is lower than 70 percent, the two single-stranded nucleic acid molecules are considered nonhomologous and any hybridisation is considered nonstringent. (13 Oct 1997) |
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| stringency | conscientious attention to rules and details |
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| stringency | a state occasioned by scarcity of money and a shortage of credit |
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