| CIMS | chemical ionization mass spectrometry |
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| EDS | edema disease of swine; egg drop syndrome; Ehlers-Danlos syndrome; Emery-Dreifus syndrome; energy-di... |
| EIMS | electron ionization mass spectrometry |
| ES | ejection sound; elastic stocking; electrical stimulus, electrical stimulation; electroshock; emergen... |
| GC-MS | gas chromatography-mass spectrometry |
| MS/MS | mass spectrometry and -tandem mass spectrometry |
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| CE-LIF | Capillary electrophoresis with laser-induced fluorescence detection |
| EDXRF | Energy Dispersive X-Ray Fluorescence |
| EGFP | Enhanced Green Fluorescence Protein |
| FP | FLuorescence polarization |
| spectrometry, fluorescence | Measurement of the intensity and quality of fluorescence. (12 Dec 1998) |
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| clinical spectrometry | Spectroscopic determination of the types and amounts of various substances in living tissue or fluid from a living body. Synonym: clinical spectrometry. Origin: bio-+ L. Spectrum, an image, + G. Metron, measure (05 Mar 2000) |
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| spectrometry | The procedure of observing and measuring the wavelengths of light or other electromagnetic emissions. (05 Mar 2000) |
| spectrometry, gamma | Determination of the energy distribution of gamma rays emitted by nuclei. (12 Dec 1998) |
| spectrometry, mass, fast atom bombardment | A mass spectrometric technique that is used for the analysis of a wide range of biomolecules, such as glycoalkaloids, glycoproteins, polysaccharides, and peptides. Positive and negative fast atom bombardment spectra are recorded on a mass spectrometer fitted with an atom gun with xenon as the customary beam. The mass spectra obtained contain molecular weight recognition as well as sequence information. (12 Dec 1998) |
| spectrometry, mass, matrix-assisted laser desorption-ionization | A mass spectrometric technique that is used for the analysis of large biomolecules. Analyte molecules are embedded in an excess matrix of small organic molecules that show a high resonant absorption at the laser wavelength used. The matrix absorbs the laser energy, thus inducing a soft disintegration of the sample-matrix mixture into free (gas phase) matrix and analyte molecules and molecular ions. In general, only molecular ions of the analyte molecules are produced, and almost no fragmentation occurs. This makes the method well suited for molecular weight determinations and mixture analysis. (12 Dec 1998) |
| spectrometry, mass, secondary ion | A mass-spectrometric technique that is used for microscopic chemical analysis. A beam of primary ions with an energy of 5-20 kiloelectronvolts (kev) bombards a small spot on the surface of the sample under ultra-high vacuum conditions. Positive and negative secondary ions sputtered from the surface are analyzed in a mass spectrometer in regards to their mass-to-charge ratio. (12 Dec 1998) |
| spectrometry, X-ray emission | Identification and measurement of concentration of elements based on the fact that X-rays emitted by an excited element have a wavelength characteristic of that element and an intensity related to its concentration. It includes fluorescence, or secondary-emission, X-ray spectrometry, in which the specimen is irradiated by X-rays. Primary-emission x-ray spectrometry, in which the specimen is bombarded by electrons, is a specific type of X-ray emission spectrometry known as electron probe microanalysis. (12 Dec 1998) |
| ratio imaging fluorescence microscopy | <procedure> A method of measurement of intracellular pH or intracellular calcium levels, using a fluorescent probe molecule (see fura-2), in which the two different excitation wavelengths are used and the emitted light levels compared. If emission at one wavelength is sensitive to the intracellular ion level and emission at the other wavelength is not, then standardisation for intracellular probe concentration, efficiency of light collection, inactivation of probe and thickness of cytoplasm can all be performed automatically. (17 Dec 1997) |
| microscopy, fluorescence | Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilises antibodies that are labelled with fluorescent dye. (12 Dec 1998) |
| Eranko's fluorescence stain | <technique> Exposure of frozen sections to formaldehyde which produces a strong yellow-green fluorescence from cells containing norepinephrine. (05 Mar 2000) |
| fluorescence | <chemistry, physics> The emission of one or more photons by a molecule or atom activated by the absorption of a quantum of electro magnetic radiation. Typically the emission, that is of longer wavelength than the excitatory radiation, occurs within 10exp 8 seconds: phosphorescence is a phenomenon with a longer or much longer delay in re radiation. Note that rays, X-rays, UV, visible light and IR radiations may all stimulate fluorescence. (25 Jun 1999) |
| fluorescence-activated cell sorter | <technique> Flow cytometry is an emerging technique which holds great promise for the separation, classification and quantitation of blood cells and antibodies which affect blood cells. Complex computerised instruments are used to pass a monocellular stream of cells, platelets or other microscopic particulate elements through a beam of laser light. The cells are categorised first by size and then computer analysed to sort the mixture of cellular elements into cell type by size. Cells are labelled with fluorescent dye and then passed, in suspending medium, through a narrow dropping nozzle so that each cell is in a small droplet. A laser based detector system is used to excite fluorescence and droplets with positively fluorescent cells are given an electric charge. Charged and uncharged droplets are separated as they fall between charged plates and so collect in different tubes. The machine can be used either as an analytical tool, counting the number of labelled cells in a population or to separate the cells for subsequent growth of the selected population. Further sophistication can be built into the system by using a second laser system at right angles to the first to look at a second fluorescent label or to gauge cell size on the basis of light scatter. The great strength of the system is that it looks at large numbers of individual cells and makes possible the separation of populations with, for example: particular surface properties. Tabulation of counted data in conjunction with size analysis enables determination of relative percentages of each specific cellular subset for which monoclonal antibody conjugates are utilised, even when the size of the cell is identical to other subset species. Flow cytometry is a slightly imprecise but common term for the use of the Fluorescence-activated Cell Sorter (FACS). (01 Dec 1998) |
| fluorescence-activated cell sorting | <technique> A technique for separating and sorting cells marked with a fluorescent label based on how much they fluoresce at a particular wavelength. (12 Jan 1998) |
| fluorescence energy transfer | <technique> Transfer of energy from one fluorochrome to another. The emission wavelength of the fluorochrome excited by the incident light must approximately match the excitation wavelength of the second fluorochrome. If light at the second emission wavelength is detected, it implies that the two fluorochromes were physically within a few nanometres. Used as a technique to probe protein or cell interactions. (25 Jun 1999) |
| fluorescence immunoassay | <technique> A sensitive technique which uses fluorescein, a fluorescent molecule, to measure the antigen or antibody concentration in a solution. (09 Oct 1997) |
Synonyms : Fluorescence Spectrometry, Spectrophotometry, Fluorescence, Spectroscopy, Fluorescence
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