| SSCP | single-stranded conformational polymorphism |
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| SSB | short spike burst; sicca syndrome B; single-strand break; single-stranded binding [protein]; stereos... |
| SSC | single-strand conformational [analysis]; sister strand crossover; somatosensory cortex; standard sal... |
| SS | disulfide; sacrosciatic; saline soak; saline solution; saliva sample; saliva substitute; Salmonella-... |
| ss(c)DNA | single-stranded circular deoxyribonucleic acid |
| SSCP | Single Stranded Conformational Polymorphism |
|---|---|
| SSCP | Single Stranded Conformational Polymorphism analysis |
| SSCP | PCR)-single stand conformational polymorphism |
| PCR-SSCP | Polymerase chain reaction single strand conformational polymorphism |
| SSCP | Single Strand Conformational Polymorphism |
| single stranded conformational polymorphism | Technique for detecting point mutations in genes by amplifying a region of genomic DNA (using asymmetric PCR) and running the resulting product on a high quality gel. Single base substitutions can alter the secondary structure of the fragment in the gel, producing a visible shift in its mobility. (18 Nov 1997) |
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| polymorphism, single-stranded conformational | Variation occurring within a species in the conformation of denatured DNA fragments. These single-stranded DNA fragments are allowed to partially renature in a way that prevents the formation of double-stranded DNA. The fragments are run on polyacrylamide gels under various conditions to detect subtle changes in migration due to altered secondary structure. The resulting bands will align themselves if the fragments are the same, but will misalign if any point mutations are present. Sscps have been used in detecting mutations in various genes, such as oncogenes, tumour suppressor genes, and genes responsible for genetic diseases. (12 Dec 1998) |
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| single stranded binding protein | <protein> SSB's are proteins responsiblefor holding the replication fork of DNAopen while polymerases readthe templates. (09 Oct 1997) |
| single stranded DNA | <molecular biology> DNA that consists of only one chain of nucleotides rather than the two base pairing strands found in DNA in the double helix form. Parvoviridae have a single stranded DNA genome. Single stranded DNA can be produced experimentally by rapidly cooling heat denatured DNA. Heating causes the strands to separate and rapid cooling prevents renaturation. (18 Nov 1997) |
| single stranded DNA dependent ATPase | <enzyme> Probably involved in DNA replication Registry number: EC 3.6.1.- Synonym: ssdna-dependent atpase (26 Jun 1999) |
| DNA, single-stranded | A single chain of deoxyribonucleotides that occurs in some bacteria and viruses. It usually exists as a covalently closed circle. (12 Dec 1998) |
| conformational change | <cell biology> Alteration in the shape usually the tertiary structure of a protein as a result of alteration in the environment pH, temperature, ionic strength) or the binding of a ligand (to a receptor) or binding of substrate (to an enzyme). (18 Nov 1997) |
| conformational map | A graphical representation in which the dihedral angle of rotation about the alpha-carbon to carbonyl-carbon bond in polypeptides is plotted against the dihedral angle of rotation about the alpha-carbon to nitrogen bond. Synonym: conformational map. (05 Mar 2000) |
| Haworth conformational formula | <biochemistry> Of cyclic sugars, for the pyranoses, these depict those shapes (conformations) on which none, one, or two ring-atoms lie outside the plane of the ring. If there are two such atoms para to each other, they can lie 1) on opposite sides of the plane (trans), giving chair forms, or 2) on the same side of the plane (cis), giving boat forms. For beta-d-ribopyranose, the two chair forms (4C1 and 1C4) are depicted. Similarly, there are six boat conformations. If the two (trans) exoplanar atoms are meta to each other, the conformation is a skew form; if the two atoms are ortho to each other, the conformation is a half-chair form. For the furanoses, the envelope conformations have one ring-atom exoplanar. If there are three adjacent, coplanar ring-atoms (the two exoplanar ring-atoms on opposite sides of the plane), the conformations are twist forms. (05 Mar 2000) |
| RNA, double-stranded | RNA consisting of two strands as opposed to the more prevalent single-stranded RNA. most of the double-stranded segments are formed from transcription of DNA by intramolecular base-pairing of inverted complementary sequences separated by a single-stranded loop. Some double-stranded segments of RNA are normal in all organisms. (12 Dec 1998) |
| negative stranded RNA virus | <virology> Class V viruses that have an RNA genome that is complementary to the mRNA, the positive strand. They also carry the virus specific RNA polymerase necessary for the synthesis of the mRNA. Includes (Rhabdoviridae, Paramyxoviridae and Myoviridae (for example the T even phages). (18 Nov 1997) |
| double-stranded complimentary DNA | <molecular biology> A duplex DNA molecule copied from a copy DNA template. (09 Oct 1997) |
| amplified fragment length polymorphism | <technique> Invented by KeyGene, a Dutch biotech company based in Wageningen, Holland. The technique is now merchandised under licence agreement by Perkin Elmer. Selected markers are amplified in a PCR, which makes amplified fragment length polymorphism an easy and fast tool for strain identification in agriculture, botany, microbiology and animal breeding. Acronym: AFLP (05 Feb 1998) |
| balanced polymorphism | <genetics> A type of polymorphism where the many morphs are alleles. The polymorphism is maintained in a population rather than the norm of one allele eventually becoming the only allele in the population, because individuals who are heterozygous, or have two different alleles, are more adaptive (more fit) than individuals who are homozygous, or have two copies of the same allele. (09 Oct 1997) |
| genetic polymorphism | The occurrence in the same population of multiple discrete alletic states of which at least two have high frequency (conventionally of 1% or more). (05 Mar 2000) |
| restriction fragment length polymorphism | <molecular biology, technique> A method that allows familial relationships to be established by comparing the characteristic polymorphic patterns that are obtained when certain regions of genomic DNA are amplified (typically by PCR) and cut with certain restriction enzymes. The variation in the length of DNA fragments produced by a restriction endonuclease that cuts at a polymorphic locus. Such variations are generated by mutations that create or abolish recognition sites for these enzymes. This is a key tool in DNA fingerprinting, reflecting the existence of different alleles in the individual. Restriction fragment length polymorphism mapping is also used in plant breeding to see if a key trait such as disease resistance is inherited. In principle, an individual can be identified unambiquously by restriction fragment length polymorphism hence the use of restriction fragment length polymorphism in forensic analysis of blood, hair or semen). Similarly, if a polymorphism can be identified close to the locus of a genetic defect, it provides a valuable marker for tracing the inheritance of the defect. Synonym: DNA fingerprinting. Acronym: RFLP (12 Jan 1998) |
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