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| agit | agitated, agitation; shake [Lat. agita] |
|---|---|
| MLC | minimum lethal concentration; mixed leukocyte culture; mixed ligand chelate; mixed lymphocyte concen... |
| TC | target cell; taurocholate; temperature compensation; teratocarcinoma; tertiary cleavage; tetracyclin... |
| TCID | tissue culture infective dose; tissue culture inoculated dose |
| TCID50 | median tissue culture infective dose; 50% tissue culture infective dose |
| A.T.C.C. | American Type Culture Collection |
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| CFUc | Colony Forming Units in culture |
| CFU-C | Colony-forming units culture |
| CF | Culture filtrate |
| CS | Culture supernatants |
| shake culture | A culture made by inoculating a liquefied gelatin or agar medium, distributing the inoculum thoroughly by agitation, and then allowing the medium to solidify in the tube in an upright position. (05 Mar 2000) |
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| mitotic shake off method | <cell biology, procedure> A method of collecting cells in mitosis, so that the chromosomes can be examined and the karyotype determined. Many cultured cells round up during mitosis and so become less firmly attached to the culture substratum. Cells in mitosis thus can be removed into suspension by gentle shaking of the culture vessel, leaving the nonmitotic cells still attached. The number of cells that are in mitosis is usually increased by using a drug, such as colcemid that blocks mitosis at metaphase. (18 Nov 1997) |
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| shake | 1. To cause to move with quick or violent vibrations; to move rapidly one way and the other; to make to tremble or shiver; to agitate. "As a fig tree casteth her untimely figs, when she is shaken of a mighty wind." (Rev. Vi. 13) "Ascend my chariot; guide the rapid wheels That shake heaven's basis." (Milton) 2. To move from firmness; to weaken the stability of; to cause to waver; to impair the resolution of. "When his doctrines grew too strong to be shook by his enemies, they persecuted his reputation." (Atterbury) "Thy equal fear that my firm faith and love Can by his fraud be shaken or seduced." (Milton) 3. To give a tremulous tone to; to trill; as, to shake a note in music. 4. To move or remove by agitating; to throw off by a jolting or vibrating motion; to rid one's self of; generally with an adverb, as off, out, etc.; as, to shake fruit down from a tree. "Shake off the golden slumber of repose." (Shak) "'Tis our fast intent To shake all cares and business from our age." (Shak) "I could scarcely shake him out of my company." (Bunyan) To shake a cask, to luff up in the wind, causing the sails to shiver. Origin: OE. Shaken, schaken, AS. Scacan, sceacan; akin to Icel. & Sw. Skaka, OS. Skakan, to depart, to flee. Cf. Shock. 1. The act or result of shaking; a vacillating or wavering motion; a rapid motion one way and other; a trembling, quaking, or shivering; agitation. "The great soldier's honor was composed Of thicker stuff, which could endure a shake." (Herbert) "Our salutations were very hearty on both sides, consisting of many kind shakes of the hand." (Addison) 2. A fissure or crack in timber, caused by its being dried too suddenly. 3. A fissure in rock or earth. 4. A rapid alternation of a principal tone with another represented on the next degree of the staff above or below it; a trill. 5. One of the staves of a hogshead or barrel taken apart. 6. A shook of staves and headings. 7. <zoology> The redshank; so called from the nodding of its head while on the ground. No great shakes, of no great importance. The shakes, the fever and ague. Source: Websters Dictionary (01 Mar 1998) |
| shake test | A test for foetal pulmonary maturity, determined by the ability of pulmonary surfactant in amniotic fluid to generate stable foam in the presence of ethanol after mechanical agitation. Synonym: shake test. (05 Mar 2000) |
| American Type Culture Collection | <cell culture> A key resource for cultured cells, located in Rockville, USA. (12 Dec 1998) |
| animal cell culture | <cell culture> Mammalian cells are fragile and harder to grow than other cell types, but their large-scale culturing is an economic boon because it allows for the production of proteins that are otherwise difficult or expensive or unethical to extract from living organisms. The cells are immobilised on a substrate and then perfused with culture medium, The cells are in a free suspension which is very gently mixed and aerated. (12 Nov 1997) |
| anoxic culture | A culture of anaerobicmicrobes which use inorganic substances other thanoxygen as their terminal electron acceptors. (09 Oct 1997) |
| anther culture | A plant culturing technique in which immature pollen is made to divide andgrow into tissue (either callus or embryonic tissue) in either aliquidmedium or on solid media. Pollen-containing anthers are removed from aflower and put in a culture medium, some microspheres survive and developinto tissue. If embryonic tissue develops, its put in a medium favorablefor shoot and root development, if its callus tissue, its put in asolution of hormones that will spur it to differentiate and develop shootand root tissue. (09 Oct 1997) |
| axenic culture | <cell culture, microbiology> A culture that contains only one microbial species. (02 Jan 1998) |
| batch culture | A closed system culture of microorganisms with specific nutrient types, temperature, pressure, aeration, and other environmental conditions, where only a few generations are allowed to grow before all nutrients are used up. Compare: continuous culture. (09 Oct 1997) |
| blood culture | <investigation, microbiology> A test which involves the incubation of a blood specimen overnight to determine if bacteria are present. (27 Sep 1997) |
| cell culture | General term referring to the maintenance of cell strains or lines in the laboratory. (18 Nov 1997) |
| roll-tube culture | A culture in a tube of medium which has been melted and allowed to solidify while the tube is being spun; the inside of the tube is thereby coated with a thin layer of solidified medium. (05 Mar 2000) |
| mixed lymphocyte culture | <investigation> Test to determine whether a patients and donor's white blood cells interact adversely. Often used to determine whether a person would be a suitable bone marrow donor for a particular patient. (16 Dec 1997) |
| mixed lymphocyte culture test | Measure of histocompatibility at the hl-a locus. Peripheral blood lymphocytes from two individuals are mixed together in tissue culture for several days. Lymphocytes from incompatible individuals will stimulate each other to proliferate significantly (measured by tritiated thymidine uptake) whereas those from compatible individuals will not. In the one-way mlc test, the lymphocytes from one of the individuals are inactivated (usually by treatment with mitomycin c or radiation) thereby allowing only the untreated remaining population of cells to proliferate in response to foreign histocompatibility antigens. (12 Dec 1998) |
| confluent culture | <cell biology> A cell culture in which all the cells are in contact and the entire surface of the culture vessel is covered. It is also often used with the implication that the cells have also reached their maximum density, though confluence does not necessarily mean that division will cease or that the population will not increase in size. (18 Nov 1997) |
| shake culture |
a culture made by inoculating warm liquid agar culture medium in a tube and shaking to distribute contents evenly. Incubation of the resolidified culture allows the development of separated colonies; especially applicable to obligate anaerobes.
Ãâó: www.merckmedicus.com/pp/us/hcp/thcp_dorlands_conte...
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| shake culture |
An agitated suspension in culture providing adequate aeration for cells in the liquid medium. Usually an Erlenmeyer flask containing the culture is attached to a horizontal or platform shaker, or agitated with a magnetic stirrer.
Ãâó: www.fao.org/docrep/003/X3910E/X3910E22.htm
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