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shadow-cast replica <microscopy> A replica which has been shadowed.
See: shadowing.
(05 Aug 1998)
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replica A specimen for electron microscopic examination obtained by coating a crystalline array or other virus material with carbon; the mold (the replica) obtained after the viral material has been dissolved provides details of structure and arrangement.
Origin: It., fr. L.L. Re-plico, to fold back
(05 Mar 2000)
replica methods <technique> Methods in the preparation of specimens for transmission electron microscopy. The specimen (for example: a piece of freeze fractured tissue) is shadowed with metal and coated with carbon and then the tissue is digested away. The replica is then picked up on a grid and it is the replica that is examined in the microscope.
(18 Nov 1997)
replica plate A technique in which the pattern of bacterial colonies on a culture plate is copied using sterile filter paper, and then the paper is pressed against a second sterile plate. The new plate is infected with cells in the same relative positions as the colonies in the original plate. Usually the new plate is tested quite destructively for some property. The ones with the best result are identified, and the corresponding group of organisms on the original plate can be identified because they are in the equivalent place.
(14 Nov 1997)
replica plating <technique> Technique for testing the genetic characteristics of bacterial colonies. A dilute suspension of bacteria is first spread, in a petri dish, on agar containing a medium expected to support the growth of all bacteria, the master plate. Each bacterial cell in the suspension is expected to give rise to a colony. A sterile velvet pad, the same size as the petri dish, is then pressed onto it, picking up a sample of each colony. The bacteria can then be stamped onto new sterile petri dishes, plates, in the identical arrangement. The media in the new plates can be made up to lack specific nutritional requirements or to contain antibiotics. Thus colonies can be identified that cannot grow without specific nutrients or that are antibiotic resistant and cells with mutations in particular genes can be isolated.
(18 Nov 1997)
replica techniques Methods of preparing tissue specimens for visualization using an electron microscope, usually a scanning electron microscope. The methods involve the creation of exact copies of the specimens by making a mold or cast (i.e., replica) of the specimen.
(12 Dec 1998)
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