| ¿µ¹® | saturation | ÇÑ±Û | Æ÷È, Æ÷ȵµ, Æ÷ȼ±·®, äµµ |
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||
| FISH | Fluorescence In Situ Hybridization |
|---|---|
| CISH | competitive in situ hybridization |
| FISH | fluorescence in situ hybridization |
| FLASH | fast low angle shot; fluorescence in situ hybridization |
| ISH | icteric serum hepatitis; in situ hybridization; internal self helper; International Society of Hemat... |
| CGH | Comparative genome hybridization |
|---|---|
| FISH | Filter In Situ Hybridization |
| FISH | Fluorescence In Situ Hybridization |
| GISH | Genomic in situ hybridization |
| 3H | Hybridization of |
| saturation | 1. The act of saturating, or the state of being saturating; complete penetration or impregnation. 2. <chemistry> The act, process, or result of saturating a substance, or of combining it to its fullest extent. 3. <optics> Freedom from mixture or dilution with white; purity; said of colours. The degree of saturation of a colour is its relative purity, or freedom from admixture with white. Origin: L. Saturatio: cf. F. Saturation. Source: Websters Dictionary (01 Mar 1998) |
|---|---|
| saturation analysis | General term for an assay in which a binder competes for labelled versus unlabelled ligand; following separation of free and bound ligand, the ligand (the analyte assayed) is quantitated by relating bound and unbound ratios to known standards. See: enzyme-linked immunosorbent assay, radioreceptor assay, immunoassay, enzyme-multiplied immunoassay technique, radioimmunoassay. Synonym: displacement analysis, saturation analysis. (05 Mar 2000) |
| saturation index | An indication of the relative concentration of haemoglobin in the red blood cells, calculated as: grams of haemoglobin per 100 ml (expressed as percent of normal) ÷ haematocrit value (expressed as percent of normal) = saturation index The normal index for adults and infants is 0.97 to 1.02; in primary and secondary anaemia, the index is usually considerably less than 0.97. (05 Mar 2000) |
| saturation of receptors | Saturation, the state in which all receptors are effectively occupied all the time, can be said to occur in a simple binding equilibrium when the concentration of ligand is more than 5 times the Kd value, although strictly this will only be true at infinite ligand concentration. (18 Nov 1997) |
| secondary saturation | A technique of nitrous oxide anaesthesia consisting of an abrupt curtailment of the oxygen in the inhaled mixture to produce a deep plane of anaesthesia, following which oxygen is administered to correct hypoxia. (05 Mar 2000) |
| cell hybridization | Fusion of two or more dissimilar cells, leading to formation of a synkaryon. (05 Mar 2000) |
| cross hybridization | Annealing of a DNA probe to an imperfectly matching DNA molecule. (05 Mar 2000) |
| protein hybridization | The formation of a protein consisting of two or more polypeptide chains from separate and different polypeptide chains. (12 Dec 1998) |
| hybridization | <molecular biology> The process of joining two complementary strands of DNA or one each of DNA and RNA to form a double-stranded molecule. Technique in which single stranded nucleic acids are allowed to interact so that complexes or hybrids, are formed by molecules with sufficiently similar, complementary sequences. By this means the degree of sequence identity can be assessed and specific sequences detected. The hybridisation can be carried out in solution or with one component immobilised on a gel or, most commonly, nitrocellulose paper. Hybrids are detected by various means: visualisation in the electron microscope, by radioactively labelling one component and removing noncomplexed DNA or by washing or digestion with an enzyme that attacks single stranded nucleic acids and finally estimating the radioactivity bound. Hybridisations are done in all combinations: DNA DNA (DNA can be rendered single stranded by heat denaturation), DNA RNA or RNA RNA. In situ hybridisations involve hybridising a labelled nucleic acid (often labelled with a fluorescent dye) to suitably prepared cells or histological sections. This is used particularly to look for specific transcription or localisation of genes to specific chromosomes (FISH analysis). <zoology> The mating of individuals from different species or sub-species. (13 Oct 1997) |
| somatic cell hybridization | Production of a heterokaryon. (05 Mar 2000) |
| nucleic acid hybridization | Widely used technique which exploits the ability of complementary sequences in single-stranded dnas or rnas to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (kendrew, encyclopedia of molecular biology, 1994, p503; dorlands, 28th ed, p781) (12 Dec 1998) |
| DNA hybridization | A technique used to determine the relatedness of microorganisms by the speed and efficiency of the reassociation of single-stranded DNA to form double-stranded DNA when one of the strands originates from one organism and the other strand from another organism; occurs when the base sequences are complementary or nearly so. (05 Mar 2000) |
| in situ hybridization | <molecular biology> The use of a DNA or RNA probe to detect the complementary DNA sequence. (12 Dec 1998) |
| in situ nucleic acid hybridization | <molecular biology> The use of a DNA or RNA probe to detect the complementary DNA sequence. (12 Dec 1998) |
| overlap hybridization | A procedure to find and sequence a gene whose approximate position in a chromosome is known by classical genetic linkage studies. Starting with the known sequence of a gene shown by classical genetics to be near to the novel gene, new clones are picked from a genomic library by hybridisation with a short probe generated from the appropriate end of the known sequence. The new clones are then sequenced, new probes generated and the process repeated until the gene of interest is reached. (18 Nov 1997) |
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