| MAP | Mean Arterial Pressure |
|---|---|
| CEM | computerized electroencephalographic map; conventional transmission electron microscope |
| ISM | information sources map [of UMLS]; International Society of Microbiologists; intersegmental muscle |
| MAP | malignant atrophic papulosis; mandibular angle plane; maturation-activated protein; maximal aerobic ... |
| MDFD | map-dot-fingerprint dystrophy |
| MAP-KKK | MAP kinase kinase kinase |
|---|---|
| ARDRA | Amplified ribosomal DNA restriction analysis |
| CR | Caloric restriction |
| DR | Diet restriction |
| DR | Dietary restriction |
| restriction map | <molecular biology> Map of DNA showing the position of sites recognised and cut by various restriction endonucleases. (12 Jan 1998) |
|---|---|
| restriction mapping | Use of restriction endonucleases to analyze and generate a physical map of genomes or genes. The nucleotide sequence determined is often then translated into an amino acid sequence, providing a means for sequencing the protein for which the gene codes, or for which the mRNA is a messenger. (12 Dec 1998) |
| cell cycle restriction point | <cell biology, molecular biology> A point, late in G1, after which the cell must, normally, proceed through to division at its standard rate. (26 Mar 1998) |
|---|---|
| restriction | 1. The process with which foreign DNA that has been introduced into a prokaryotic cell becomes ineffective. 2. A limitation. (05 Mar 2000) |
| restriction endonuclease | <enzyme, molecular biology> Class of bacterial enzymes that cut DNA at specific sites. In bacteria their function is to destroy foreign DNA, such as that of bacteriophages (host DNA is specifically modified at these sites). Type I restriction endonucleases occur as a complex with the methylase and a polypeptide that binds to the recognition site on DNA. They are often not very specific and cut at a remote site. Type II restriction endonucleases are the classic experimental tools. They have very specific recognition and cutting sites. The recognition sites are short, 4-8 nucleotides and are usually palindromic sequences. Because both strands have the same sequence running in opposite directions the enzymes make double stranded breaks, which, if the site of cleavage is off centre, generates fragments with short single stranded tails, these can hybridise to the tails of other fragments and are called sticky ends. They are generally named according to the bacterium from which they were isolated (first letter of genus name and the first two letters of the specific name). The bacterial strain is identified next and multiple enzymes are given Roman numerals. For example the two enzymes isolated from the R strain of E. Coli are designated Eco RI and Eco RII. (10 Mar 1998) |
| restriction enzyme | <enzyme, molecular biology> Class of bacterial enzymes that cut DNA at specific sites. In bacteria their function is to destroy foreign DNA, such as that of bacteriophages (host DNA is specifically modified at these sites). Type I restriction endonucleases occur as a complex with the methylase and a polypeptide that binds to the recognition site on DNA. They are often not very specific and cut at a remote site. Type II restriction endonucleases are the classic experimental tools. They have very specific recognition and cutting sites. The recognition sites are short, 4-8 nucleotides and are usually palindromic sequences. Because both strands have the same sequence running in opposite directions the enzymes make double stranded breaks, which, if the site of cleavage is off centre, generates fragments with short single stranded tails, these can hybridise to the tails of other fragments and are called sticky ends. They are generally named according to the bacterium from which they were isolated (first letter of genus name and the first two letters of the specific name). The bacterial strain is identified next and multiple enzymes are given Roman numerals. For example the two enzymes isolated from the R strain of E. Coli are designated Eco RI and Eco RII. (10 Mar 1998) |
| restriction enzyme cutting site | <molecular biology> A specific nucleotide sequence of DNA at which a particular restriction enzyme cuts the DNA. Some sites occur frequently in DNA (for example, every several hundred basepairs), others much less frequently (rare-cutter, for example, every 10,000 base pairs). (10 Mar 1998) |
| restriction enzyme, endonuclease | A protein that recognises specific, short nucleotide sequences and cuts DNA at those sites. Bacteria contain over 400 such enzymes that recognise and cut over 100 different DNA sequences. See restriction enzyme cutting site. (05 Mar 2000) |
| restriction fragment | <molecular biology> The fragments of DNA generated by digesting DNA with a specific restriction endonuclease. Each of the fragments ends in a site recognised by that specific enzyme. (10 Mar 1998) |
| restriction fragment length polymorphism | <molecular biology, technique> A method that allows familial relationships to be established by comparing the characteristic polymorphic patterns that are obtained when certain regions of genomic DNA are amplified (typically by PCR) and cut with certain restriction enzymes. The variation in the length of DNA fragments produced by a restriction endonuclease that cuts at a polymorphic locus. Such variations are generated by mutations that create or abolish recognition sites for these enzymes. This is a key tool in DNA fingerprinting, reflecting the existence of different alleles in the individual. Restriction fragment length polymorphism mapping is also used in plant breeding to see if a key trait such as disease resistance is inherited. In principle, an individual can be identified unambiquously by restriction fragment length polymorphism hence the use of restriction fragment length polymorphism in forensic analysis of blood, hair or semen). Similarly, if a polymorphism can be identified close to the locus of a genetic defect, it provides a valuable marker for tracing the inheritance of the defect. Synonym: DNA fingerprinting. Acronym: RFLP (12 Jan 1998) |
| restriction length polymorphism | Fragment length polymorphism, the existence of allelic forms recognizable by the length of fragments that result when the nucleotide chain is treated by a specific restriction enzyme that cleaves wherever a particular sequence of nucleotides occurs. A mutation in this sequence changes cleaving and hence the number of fragments. (05 Mar 2000) |
| restriction methylation | The enzymatic addition of methyl groups to selected adenine and cytosine residues to protect from hydrolysis by certain restriction enzymes. (05 Mar 2000) |
| restriction nuclease | <enzyme, molecular biology> Class of bacterial enzymes that cut DNA at specific sites. In bacteria their function is to destroy foreign DNA, such as that of bacteriophages (host DNA is specifically modified at these sites). Type I restriction endonucleases occur as a complex with the methylase and a polypeptide that binds to the recognition site on DNA. They are often not very specific and cut at a remote site. Type II restriction endonucleases are the classic experimental tools. They have very specific recognition and cutting sites. The recognition sites are short, 4-8 nucleotides and are usually palindromic sequences. Because both strands have the same sequence running in opposite directions the enzymes make double stranded breaks, which, if the site of cleavage is off centre, generates fragments with short single stranded tails, these can hybridise to the tails of other fragments and are called sticky ends. They are generally named according to the bacterium from which they were isolated (first letter of genus name and the first two letters of the specific name). The bacterial strain is identified next and multiple enzymes are given Roman numerals. For example the two enzymes isolated from the R strain of E. Coli are designated Eco RI and Eco RII. (10 Mar 1998) |
| restriction site | A sequence in DNA that can be recognised and cut by a specific restriction enzyme. (12 Dec 1998) |
| restriction-site polymorphism | DNA polymorphism in which the sequence of one form of the polymorphism contains a recognition site for a particular endonuclease, but the sequence of the other form lacks such a site. (05 Mar 2000) |
| MHC restriction | <immunology> Restriction on interaction between cells of the immune system because of the requirement to recognise foreign antigen is association with MHC antigens (major histocompatibility antigens). Thus, cytotoxic T-cells will only kill virally infected cells that have the same Class I antigens as themselves, whereas helper T-cells respond to foreign antigen associated with Class II antigens. (18 Nov 1997) |
| host restriction-modification | A bacterial system where the bacterium is able to destroy invading DNA from a bacteriophage (virus which infects bacteria) while at the same time preventing the destruction of their own DNA. The phage DNA is cleaved by a restriction enzyme made by the bacterium, the bacterial DNA is modified (usually with methylation) so that the enzyme will not destroy it. (09 Oct 1997) |
Synonyms : Analysis, Restriction Enzyme, Enzyme Analysis, Restriction, Restriction Enzyme Analysis, Analyses, Restriction Enzyme, Endonuclease Mappings, Restriction, Enzyme Analyses, Restriction, Enzyme Mappings, Restriction, Mapping, Restriction, Mappings, Restriction
| restriction map |
A description of restriction endonuclease cleavage sites within a piece of DNA. Generating such a map is usually the first step in characterizing an unknown DNA, and a prerequisite to manipulating it for other purposes. Typically, restriction enzymes that cleave DNA infrequently (eg those with 6 bp recognition sites) and are relatively inexpensive are used to produce at a map.
Ãâó: www.geocities.com/bioinformaticsweb/genomicglossar...
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| restriction map |
The linear array of restriction endonuclease sites on a DNA molecule. See mapping.
Ãâó: www.fao.org/docrep/003/X3910E/X3910E21.htm
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| restriction map |
A genetic map of DNA showing the relative positions of restriction enzyme cleavage sites.
Ãâó: www.modernhumanorigins.com/r.html
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| restriction map |
is a linear array of sites on DNA cleaved by various restriction enzymes.
Ãâó: www.hgsc.bcm.tmc.edu/docs/HGSC_glossary.html
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| restriction map |
A map of a DNA sequence showing the restriction sites of one or more restriction enzymes.
Ãâó: www.promega.com/guides/re_guide/glossary/glossary1...
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