| NRRL | Northern Regional Research Laboratory |
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| NT | Northern Territory |
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| NFM | Northern fowl mites |
| SB | Southern blotting |
| WB | Westen blotting |
| WLB | Western Ligand Blotting |
| blotting, northern | Detection of RNA that has been electrophoretically separated and immobilised by blotting on nitrocellulose or other type of paper or nylon membrane. (12 Dec 1998) |
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| blotting | <molecular biology, procedure> General term for the transfer of protein, RNA or DNA molecules from a relatively thick acrylamide or agarose gel or to a paper like membrane (usually nylon or nitrocellulose) by capilliarity or an electric field, preserving the spatial arrangment. Once on the membrane, the molecules are immobilised, typically by baking or by ultra violet irradiation and can then be detected at high sensitivity by hybridisation (in the case of DNA & RNA) or antibody labelling (in the case of protein). RNA blots are called Northern blots, DNA blots, Southern, protein blots, Western. (18 Nov 1997) |
| blotting, southern | A method (first developed by e.m. Southern) for detection of DNA that has been electrophoretically separated and immobilised by blotting on nitrocellulose or other type of paper or nylon membrane. (12 Dec 1998) |
| blotting, western | Identification of proteins or peptides that have been electrophoretically separated by blotting and transferred to strips of nitrocellulose paper. The blots are then detected by radiolabelled antibody probes. (12 Dec 1998) |
| Western blotting | <molecular biology> An electroblotting method in which proteins are transferred from a gel to a thin, rigid support (nitrocellulose) and detected by binding of labelled antibody. See: blots. (18 Nov 1997) |
| Southern blotting | <molecular biology, procedure> General term for the transfer of protein, RNA or DNA molecules from a relatively thick acrylamide or agarose gel or to a paper like membrane (usually nylon or nitrocellulose) by capilliarity or an electric field, preserving the spatial arrangment. Once on the membrane, the molecules are immobilised, typically by baking or by ultra violet irradiation and can then be detected at high sensitivity by hybridisation (in the case of DNA & RNA) or antibody labelling (in the case of protein). RNA blots are called Northern blots, DNA blots, Southern, protein blots, Western. (18 Nov 1997) |
| africa, northern | The geographical area of africa comprising algeria, egypt, libya, morocco, and tunisia. It includes also the vast deserts and oases of the sahara. It is often referred to as north africa, french-speaking africa, or the magreb. (12 Dec 1998) |
| blot, northern | A technique in molecular biology, used mainly to separate and identify pieces of RNA. Called a Northern blot only because it is similar to a Southern blot (which is named after its inventor, the British biologist M.E. Southern). (12 Dec 1998) |
| northern | 1. Of or pertaining to the north; being in the north, or nearer to that point than to the east or west. 2. In a direction toward the north; as, to steer a northern course; coming from the north; as, a northern wind. Northern diver. <zoology> See Loon. Northern lights. See Aurora borealis, under Aurora. <botany> Northern spy, an excellent American apple, of a yellowish colour, marked with red. Origin: AS. Northerne. Source: Websters Dictionary (01 Mar 1998) |
| Northern blot | <molecular biology, procedure> An electroblotting method in which RNA is transferred to a filter and detected by hybridisation to (32)P labelled RNA or DNA. (18 Nov 1997) |
| Northern blot analysis | A procedure similar to the Southern blot analysis, used mostly to separate and identify RNA fragments; typically via transferring RNA fragments from an agarose gel to a nitrocellulose filter followed by detection with a suitable probe. Origin: coined to distinguish it from eponymic Southern blot a. (05 Mar 2000) |
| northern territory | A territory in north central Australia, east of the state of queensland. Its capital is darwin. Originally a part of new south wales, it was annexed to south Australia in 1863, entered the commonwealth as part of it in 190l, transferred to the commonwealth in 1911, divided in 1927, but was reestablished in 1931. It was granted self government within the commonwealth of Australia in 1978. (12 Dec 1998) |
| Northern blotting method |
A blot analysis technique for analyzing a small portion of RNA. Operationally, this test is identical to Southern blotting except for the target (RNA) and the specific reagents used.
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