| CSLM | confocal scanning microscopy |
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| EM | 1) Erythro-Mycin 2) Electron Microscopy |
| AEM | Academic Emergency Medicine [journal]; analytical electron microscopy; ambulatory electrocardiograph... |
| ARM | adrenergic receptor material; aerosol rebreathing method; ambulatory renal monitor; anorectal manome... |
| ATEM | analytic transmission electron microscopy |
| CMTF | Confocal Microscopy Through Focusing |
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| TSCM | Tandem Scanning Confocal Microscopy |
| CLSM | Confocal Laser Scanning Microscope |
| CSLM | Confocal Scanning Laser Microscope |
| LSCM | Laser Scanning Confocal Microscope |
| microscopy, confocal | A light microscopic technique in which only a small spot is illuminated and observed at a time. An image is constructed through point-by-point scanning of the field in this manner. Light sources may be conventional or laser, and fluorescence or transmitted observations are possible. (12 Dec 1998) |
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| confocal microscopy | <procedure> A system of (usually) epifluorescence light microscopy in which a fine laser beam of light is scanned over the object through the objective lens. The technique is particularly good at rejecting light from outside the plane of focus and so produces higher effective resolution than is normally achieved. (18 Nov 1997) |
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| confocal | See: confocal microscope. (05 Mar 2000) |
| confocal microscope | <instrument> A microscope that allows the observer to visualise objects in a single plane of focus, thereby creating a sharper image (usually the objects are fluorescent molecules); a refinement of this microscope uses optical sectioning and a computer to record serial sections. This permits three-dimensional reconstruction. (05 Mar 2000) |
| confocal optics | <microscopy> A (microscope) optical system in which the condenser and objective lenses both focus onto one single point in the specimen. Generally, the image of a pinhole source is focused onto a point in the specimen, and that point is focused by the objective lens onto a point detector or through a mask with a pinhole aperture. With confocal optics, the Abbe limit of resolution can be exceeded since only a limited region of the specimen is viewed at any onetime. (05 Aug 1998) |
| aperture for electron microscopy | <technique> Anode aperture: The opening in the accelerating voltage anode shield of the electron gun through which the electrons must pass to irradiate the specimen. Condenser aperture: An opening in the condenser lens controlling the number of electrons entering the lens and the angular aperture of the electron beam. The angular aperture can also be controlled by the condenser lens current. Physical objective aperture: A metallic diaphragm, with a small central hole, used to limit the cone of electrons accepted by the objective lens. This improves image-contrast since highly scattered electrons are prevented from arriving at the Gaussian image plane and therefore cannot contribute to background fog. Aplanatic. Free from spherical aberration and coma. (05 Aug 1998) |
| bright field microscopy | <technique> Optical microscopy, in which absorption to a great extent and diffraction to a minor extent give rise to the image, as opposed to phase contrast or interference methods of microscopy. (18 Nov 1997) |
| ratio imaging fluorescence microscopy | <procedure> A method of measurement of intracellular pH or intracellular calcium levels, using a fluorescent probe molecule (see fura-2), in which the two different excitation wavelengths are used and the emitted light levels compared. If emission at one wavelength is sensitive to the intracellular ion level and emission at the other wavelength is not, then standardisation for intracellular probe concentration, efficiency of light collection, inactivation of probe and thickness of cytoplasm can all be performed automatically. (17 Dec 1997) |
| reflection X-ray microscopy | <technique> A method of producing enlarged images by means of X rays. In this method the radiation is totally reflected at glancing incidence from polished concave mirrors or from the curved surfaces of single crystals by Bragg reflection. The problem of aberration corrections still limits the resolution obtainable. (05 Aug 1998) |
| video microscopy | <technique> Microscopy that takes advantage of video as an imaging, image processing, analysing, or controlling device. (05 Aug 1998) |
| phase contrast microscopy | <investigation> A simple nonquantitative form of interference micoscopy of great utility in visualising live cells. Small differences in optical path length due to differences in refractive index and thickness of structures are visualised as differences in light intensity. (18 Nov 1997) |
| microscopy | <technique> The science of the interpretive use, and applications of microscopes. (05 Aug 1998) |
| microscopy, atomic force | Microscopy in which a probe systematically rides across the surface of a sample being scanned in a raster pattern. The vertical position is recorded as a spring attached to the probe rises and falls in response to peaks and valleys on the surface. A microcomputer keeps track of the vertical excursions as a function of the position of the probe in the horizontal plane and presents the sample's image. (12 Dec 1998) |
| microscopy, electron | Visual and photographic microscopy in which electron beams with wavelengths thousands of times shorter than visible light are used in place of light, thereby allowing much greater magnification. (12 Dec 1998) |
| microscopy, electron, scanning | Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point, giving the surface image a three-dimensional quality. (12 Dec 1998) |
| microscopy, electron, scanning transmission | A type of electron microscopy which scans with an extremely narrow beam that is transmitted through the sample. The detection apparatus produces an image whose brightness depends on the atomic number of the sample. It should not be confused with microscopy, electron scanning nor with microscopy, electron, transmission (see microscopy, electron). (12 Dec 1998) |
Synonyms : Confocal Laser Scanning Microscopy, Confocal Microscopies, Laser Microscopies, Laser Scanning Microscopies, Microscopies, Confocal, Microscopies, Laser, Microscopies, Laser Scanning, Microscopy, Laser, Microscopy, Laser Scanning, Scanning Microscopies, Laser
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