| AVS | aortic valve stenosis; arteriovenous shunt; auditory vocal sequencing |
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| GAWTS | genomic amplification with transcript sequencing |
| RAWTS | ribonucleic acid amplification with transcript sequencing |
| VMST | visual motor sequencing test |
| SBT | Sequencing Based Typing |
|---|---|
| SBR | Sequencing Batch Reactor |
| SBH | Sequencing by Hybridization |
| sequence ladder | The array of bands, made conspicuous by labeling, when DNA fragmented by endonucleases is subject to gel electrophoresis; corresponds to the nucleotide sequence. (05 Mar 2000) |
|---|---|
| fish ladder | A series of descending weirs which carry water around a dam or waterfall to facilitate the upstream migration of fish. (09 Oct 1997) |
| ladder | 1. A frame usually portable, of wood, metal, or rope, for ascent and descent, consisting of two side pieces to which are fastened cross strips or rounds forming steps. "Some the engines play, And some, more bold, mount ladders to the fire." (Dryden) 2. That which resembles a ladder in form or use; hence, that by means of which one attains to eminence. "Lowliness is young ambition's ladder." (Shak) Fish ladder. See Fish. Ladder beetle, a spiral marine shell of the genus Scalaria. See Scalaria. Origin: OE. Laddre, AS. Hlder, hldder; akin to OFries. Hladder, OHG.leitara, G. Leiter, and from the root of E. Lean, v. See Lean, and cf. Climax. Source: Websters Dictionary (01 Mar 1998) |
| ladder splint | A flexible splint consisting of two stout parallel wires with finer cross wires. Synonym: Cramer wire splint. (05 Mar 2000) |
| gene sequencing | Determination of the sequence of nucleotide bases in a strand of DNA. (14 Nov 1997) |
| maxam-gilbert sequencing | A lab technique used to find out the sequence of nucleotide bases in a nucleic acid (a DNA or RNA molecule). The technique involves putting copies of the nucleic acid into separate test tubes, each of which contains a chemical that will cleave the molecule at a different base (either adenine, guanine, cytosine, or thymine or uracil (the last depending on whether it is DNA or RNA)). The result is that each of the test tubes contains fragments of the nucleic acid that all end at the same base, but at different points on the molecule where the base occurs. The contents of the test tubes are then separated by size with gel electrophoresis (one gel well per test tube, four total wells), the smallest fragments will travel the farthest and the largest will travel the least far from the well. The sequence can then be determined from the picture of the finished gel by noting the sequence of the marks on the gel and from which well they came from. (09 Oct 1997) |
| Maxim-Gilbert sequencing | <molecular biology> A method of sequencing DNA using dimethyl sulfate and hydrazinolysis. (05 Mar 2000) |
| chemical sequencing | A lab technique used to determine the sequence of nucleotides in a DNA molecule. The DNA molecule is labelled with radioactive phosphorous (chemical element P), cut into fragments, and analysed through electrophoresis. (09 Oct 1997) |
| protein sequencing | Determining the sequence of amino acids in a protein, a process carried out by automated techniques. (14 Nov 1997) |
| sequencing | <molecular biology, procedure> Determination of the order of nucleotides (base sequences) in a DNA or RNA molecule or the order of amino acids in a protein. (11 Jun 1998) |
| dideoxy sequencing | The most popular method of DNA sequence determination (c.f. Maxam Gilbert sequencing). Starting with single stranded template DNA, a short complementary primer is annealed and extended by a DNA polymerase. The reaction is split into 4 tubes (called A, C, G or T) each containing a low concentration of the indicated dideoxy nucleotide, in addition to the normal deoxynucleotides. Dideoxynucleotides, once incorporated, block further chain extension and so each tube accumulates a mixture of chains of lengths determined by the template sequence. The 4 reactions are denatured and run out on an acrylamide sequencing gel in neighbouring lanes and the sequence read up the gel according to the order of the bands. (18 Nov 1997) |
| DNA sequencing | <molecular biology> Any lab technique used to find out the sequence of nucleotide bases in a DNA molecule or fragment. Examples are dideoxy sequencing and Maxam-Gilbert sequencing. (09 Oct 1997) |
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