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| CIE | Canberra interview for the elderly; cellulose ion exchange; counter-current immunoelectrophoresis; c... |
|---|---|
| IWB | indeterminate [HIV-1] Western blot; index of well being |
| WB | waist belt; washable base; washed bladder; water bottle; Wechsler-Bellevue [Scale]; weight-bearing; ... |
| WE | wax ester; Wernicke encephalopathy; western encephalitis; western encephalomyelitis; wound of entry |
| PAT | Pain Apperception Test; paroxysmal atrial tachycardia; patient; phenylaminotetrazole; physical abili... |
| WB | Western blot |
|---|---|
| WB | Western Blot analysis |
| WLB | Western Ligand Blot |
| immunoblot | immunosorbent assay and Western blot |
| CIE | Countercurrent immunoelectrophoresis |
| blot, western | A technique in molecular biology, used to separate and identify proteins. Called a Western blot merely because it has some similarity to a Southern blot (which is named after its inventor, the British biologist M.E. Southern). (12 Dec 1998) |
|---|---|
| western blot | <technique> A technique similar to Southern blotting, though it is used for proteins. (09 Oct 1997) |
| Western blot analysis | A procedure in which proteins separated by electrophoresis in polyacrylamide gels are transferred (blotted) onto nitrocellulose or nylon membranes and identified by specific complexing with antibodies that are either pre-or post-tagged with a labelled secondary protein. See: immunoblot. Synonym: Western blot, Western blotting. Origin: coined to distinguish it from eponymic Southern blot a. (05 Mar 2000) |
| rocket immunoelectrophoresis | A quantitative method for serum proteins which involves electrophoresis of antigen into a gel containing antibody; the technique is restricted to detection of antigens that move to the positive pole on electrophoresis. See: electroimmunodiffusion. Two-dimensional immunoelectrophoresis, a combination of conventional electrophoretic separation and electroimmunodiffusion; electrophoresis is first carried out, then the electrophoretic strip is placed on a second slide and an antibody-containing agarose solution is allowed to solidify adjacent to it; electrophoresis is then performed at right angles to the original separation. Synonym: crossed immunoelectrophoresis. (05 Mar 2000) |
| crossed immunoelectrophoresis | two-dimensional immunoelectrophoresis |
| immunoelectrophoresis | <technique> A two-step procedure which first involves the electrophoretic separation of proteins, followed by the linear diffusion of antibodies into the electrophoretic gel from a trough which extends through the length of the gel adjacent to the electrophoretic path. The antigen-antibody reactions produce precipitin arcs at positions where equivalence occurs. Although quantitation is subjective, an experienced eye candetermine not only the presence of the antigen but, through visual comparison to normal control sera, may discriminate relative increases or decreases of antigen by gauging the length and density of the precipitin arcs at positions established for specific antigens using known standards. (01 Dec 1998) |
| immunoelectrophoresis, two-dimensional | Immunoelectrophoresis in which a second electrophoretic transport is performed on the initially separated antigen fragments into an antibody-containing medium in a direction perpendicular to the first electrophoresis. (12 Dec 1998) |
| blot | 1. To spot, stain, or bespatter, as with ink. "The brief was writ and blotted all with gore." (Gascoigne) 2. To impair; to damage; to mar; to soil. "It blots thy beauty, as frosts do bite the meads." (Shak) 3. To stain with infamy; to disgrace. "Blot not thy innocence with guiltless blood." (Rowe) 4. To obliterate, as writing with ink; to cancel; to efface; generally with out; as, to blot out a word or a sentence. Often figuratively; as, to blot out offenses. "One act like this blots out a thousand crimes." (Dryden) 5. To obscure; to eclipse; to shadow. "He sung how earth blots the moon's gilded wane." (Cowley) 6. To dry, as writing, with blotting paper. Synonym: To obliterate, expunge, erase, efface, cancel, tarnish, disgrace, blur, sully, smear, smutch. Origin: Cf. Dan. Plette. See Blot. Source: Websters Dictionary (01 Mar 1998) |
| blot, northern | A technique in molecular biology, used mainly to separate and identify pieces of RNA. Called a Northern blot only because it is similar to a Southern blot (which is named after its inventor, the British biologist M.E. Southern). (12 Dec 1998) |
| blot, southern | A common test for checking for a match between DNA molecules. DNA fragments are separated by agarose gel electrophoresis, transferred (blotted) onto membrane filters, and hybridised with complementary radiolabelled probes. The aim is to detect specific base sequenceswith the probes. Lest all of this sound esoteric, note that in the television series The X Files a Southern blot was done (in this case, to learn if some alien virus genome had been integrated into a person's genome). The Southern blot is named after its inventor, the British biologist M.E. Southern. There is also a Northern blot and a Western blot. (12 Dec 1998) |
| southern blot | A technique used for searching for a specific DNA fragment. The process is as follows: 1. separate DNA fragments by gel electrophoresis 2. Change pH of gel to basic, thus allowing disruption of H-bonds 3. blot gel with nitrocellulose paper 4. Heat paper so as to fix DNA fragments 5. probe with labelled messenger RNA or cDNA 6. wash Complementary mRNA/cDNA fragments will have hybridised. (09 Oct 1997) |
| Southern blot analysis | A procedure to separate and identify DNA sequences; DNA fragments are separated by electrophoresis on an agarose gel, transferred (blotted) onto a nitrocellulose or nylon membrane, and hybridised with complementary (labelled) nucleic acid probes. (05 Mar 2000) |
| Northern blot | <molecular biology, procedure> An electroblotting method in which RNA is transferred to a filter and detected by hybridisation to (32)P labelled RNA or DNA. (18 Nov 1997) |
| Northern blot analysis | A procedure similar to the Southern blot analysis, used mostly to separate and identify RNA fragments; typically via transferring RNA fragments from an agarose gel to a nitrocellulose filter followed by detection with a suitable probe. Origin: coined to distinguish it from eponymic Southern blot a. (05 Mar 2000) |
| dot blot | <molecular biology, procedure> Method for detecting a specific protein or message. A spot of solution is dotted onto nitrocellulose paper, a specific antibody or probe is allowed to bind and the presence of bound antibody/probe then shown by using a peroxidase coupled second antibody, as in Western blotting or by other visualisation methods. (18 Nov 1997) |
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