| FIA | fistula in ano; fluorescent immunoassay; focal immunoassay; Freund incomplete adjuvant |
|---|---|
| FPIA | fluorescence polarization immunoassay |
| PCFIA | particle concentration of fluorescence immunoassay |
| SPIF | solid-phase immunoassay fluorescence |
| ANIA | automated nephelometric immunoassay |
| FPIA | Fluorescence Polarisation Immunoassay |
|---|---|
| FIA | Fluorescence immunoassay |
| CHEF | Clamped Homogeneous Electric Fields |
| CHEF | Contour-clamped homogeneous electric field |
| hsr | Homogeneous staining region |
| fluorescence immunoassay | <technique> A sensitive technique which uses fluorescein, a fluorescent molecule, to measure the antigen or antibody concentration in a solution. (09 Oct 1997) |
|---|---|
| fluorescence polarisation immunoassay | A technique which takes advantage of the increased polarisation (non-random propagation of emission) of fluorescent light emissions when a fluorescent labelled antigen is bound by reagent antibody. The higher the concentration of unlabelled patient antigen present in the test mixture, the less bound fluorescent antigen is present and, consequently, the lower the polarisation of the fluorescent light emission. Standard calibration yields quantitative results. (05 Mar 2000) |
| fluorescence polarization immunoassay | Fluoroimmunoassay where detection of the hapten-antibody reaction is based on measurement of the increased polarization of fluorescence-labelled hapten when it is combined with antibody. The assay is very useful for the measurement of small haptenic antigens such as drugs at low concentrations. (12 Dec 1998) |
| homogeneous | Consisting of or composed of similar elements or ingredients, of a uniform quality throughout. Origin: Gr. Genos = kind (18 Nov 1997) |
| homogeneous catalysis | <chemistry> A form of catalysis in which the catalyst is in the same physical state than the reactants, for example reactants and catalyst are all gases. (09 Jan 1998) |
| homogeneous immersion | In immersion microscopy, use of a fluid, such as oil, that has a refractive index virtually identical to that of glass, providing the highest possible numerical aperture. (05 Mar 2000) |
| homogeneous immersion objective | <microscopy> An objective to be immersed in a liquid of a certain refractive index and dispersion value as specified by the manufacturer of the objective. An oil-immersion objective, the most important type, is intended to be immersed in cedarwood oil (nD = 1.515) or in its manmade optical equivalent. A water-immersion objective is for dipping into an aqueous specimen mount. Alpha-monobromonaphthalene has such a high refractive index (nD = 1.66) that a very highly resolving objective (1.60 numerical aperture) was designed to be immersed in that liquid, for use by reflected light on metals and other opaque objects. (05 Aug 1998) |
| homogeneous radiation | Radiation consisting of a narrow band of frequencies, the same energy, or a single type of particle. (05 Mar 2000) |
| homogeneous system | In chemistry, a system whose parts cannot be mechanically separated, and is therefore uniform throughout and possesses in every part identically physical properties; e.g., a solution of sodium chloride in water. (05 Mar 2000) |
| microparticle enzyme immunoassay | A technique in which the solid-phase support consists of very small microparticles in liquid suspension. Specific reagent antibodies are covalently bound to the microparticles. Antigen, if present, is then "sandwiched" between bound antibodies and antigen-specific, enzyme-labelled antibodies. Antigen-antibody complexes are detected and quantitated by analysis of fluorescence from the enzyme-substrate interaction. Acronym: MEIA (05 Mar 2000) |
| solid phase immunoassay | Immunoassay in which the antigen or serum is bound to a solid surface, such as a microplate wall or the sides of a tube, the other reactants being free in solution. (05 Mar 2000) |
| double antibody immunoassay | A method of separating antibody-bound antigen (e.g., insulin) from free antigen by precipitating the former with antibody specific for immunoglobulin. Synonym: double antibody immunoassay, double antibody method. (05 Mar 2000) |
| immunoassay | <investigation> A process that measures and identifies a specific biological substance such as an antigen. (09 Oct 1997) |
| thin-layer immunoassay | A method for detection of antigen-antibody reactions, applicable to detection of either antigen or antibody, based on the fact that either reactant, when added to a polystyrene surface (such as a well in a polystyrene plate) is adsorbed as a thin layer and acts as an immunosorbent capable of binding with the second reactant. (05 Mar 2000) |
| enzyme immunoassay | The general term for an expanding technical arsenal of testing which allows a full range of quantitative analyses for both antigen and antibodies. These tests use colour-changed products of enzyme-substrate interaction (or inhibition) to measure the antigen-antibody reaction. Examples of EIA procedures (EMIT, ELISA, MAC, MEIA) follow. Acronym: EIA (05 Mar 2000) |
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