| GAWTS | genomic amplification with transcript sequencing |
|---|---|
| gDNA | genomic deoxyribonucleic acid |
| CGH | Comparative Genomic Hybridisation |
|---|---|
| gDNA | Genomic DNA |
| GISH | Genomic in situ hybridization |
| RLGS | Restriction Landmark Genomic Scanning |
| SB | Southern blotting |
| blotting | <molecular biology, procedure> General term for the transfer of protein, RNA or DNA molecules from a relatively thick acrylamide or agarose gel or to a paper like membrane (usually nylon or nitrocellulose) by capilliarity or an electric field, preserving the spatial arrangment. Once on the membrane, the molecules are immobilised, typically by baking or by ultra violet irradiation and can then be detected at high sensitivity by hybridisation (in the case of DNA & RNA) or antibody labelling (in the case of protein). RNA blots are called Northern blots, DNA blots, Southern, protein blots, Western. (18 Nov 1997) |
|---|---|
| blotting, northern | Detection of RNA that has been electrophoretically separated and immobilised by blotting on nitrocellulose or other type of paper or nylon membrane. (12 Dec 1998) |
| blotting, southern | A method (first developed by e.m. Southern) for detection of DNA that has been electrophoretically separated and immobilised by blotting on nitrocellulose or other type of paper or nylon membrane. (12 Dec 1998) |
| blotting, western | Identification of proteins or peptides that have been electrophoretically separated by blotting and transferred to strips of nitrocellulose paper. The blots are then detected by radiolabelled antibody probes. (12 Dec 1998) |
| genomic | Relating to a genome. (05 Mar 2000) |
| genomic DNA | <molecular biology> The DNA which is found in the organisms genome and is passed on to offspring as information necessary for survival. The phrase is used to distinguish between other types of DNA, such as found within plasmids. (09 Oct 1997) |
| genomic formula | <molecular biology> The method used to identify the number of sets of chromosomes within a cell or organism (i.e. Its ploidy). Each set is designated n so one set is n is haploid, two sets is 2n is diploid, three sets is 3n is triploid, four sets is 4n is tetraploid, etc. Also, 2n+1 is known as trisomy (i.e. Two full sets plus a third copy of one of the chromosomes). (09 Oct 1997) |
| genomic imprinting | <genetics, molecular biology> Parent specific expression or repression of genes or chromosomes in offspring. There are an increasing number of recognised chromosomal imprinting events in pathological conditions: for example preferential transmission of paternal or maternal predisposition to diabetes or atopy, preferential retention of paternal alleles in rhabdomyosarcoma, osteosarcoma, retinoblastoma and Wilm's tumour, preferential translocation to the paternal chromosome 9 of a portion of maternal chromosome 22 to form the Philadelphia chromosome of chronic myeloid leukaemia. (18 Nov 1997) |
| genomic library | <molecular biology> A collection of DNA molecules, derived from restriction fragments that have been cloned in vectors, that includes all or part of the genetic material of an organism. (18 Nov 1997) |
| Western blotting | <molecular biology> An electroblotting method in which proteins are transferred from a gel to a thin, rigid support (nitrocellulose) and detected by binding of labelled antibody. See: blots. (18 Nov 1997) |
| Southern blotting | <molecular biology, procedure> General term for the transfer of protein, RNA or DNA molecules from a relatively thick acrylamide or agarose gel or to a paper like membrane (usually nylon or nitrocellulose) by capilliarity or an electric field, preserving the spatial arrangment. Once on the membrane, the molecules are immobilised, typically by baking or by ultra violet irradiation and can then be detected at high sensitivity by hybridisation (in the case of DNA & RNA) or antibody labelling (in the case of protein). RNA blots are called Northern blots, DNA blots, Southern, protein blots, Western. (18 Nov 1997) |
| library, genomic | A collection of DNA clones made from a set of randomly generated overlapping DNA fragments representing the entire genome of an organism. As a molecular genetic sequel to john steinbeck's of mice and men , today you can have a mouse genomic library or a human genomic library. (12 Dec 1998) |
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