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  • footprinting
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footprinting <molecular biology, technique> A technique used to identify the binding site of, for example: a protein on a nucleic acid sequence.
The basic principle is to carry out a very limited hydrolysis of the DNA with or without the protein complexed and then to compare the digestion products. If a cleavage site is masked by the bound protein then the pattern of fragments when protein is present will be different and it is possible to work out, by a series of such procedures, exactly where the protein binds.
(18 Nov 1997)
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DNA footprinting <molecular biology> Technique for identifying the recognition site of DNA binding proteins:
See: footprinting.
(18 Nov 1997)
DNAse footprinting <molecular biology> A lab technique used to find out which segments of a DNA molecule are protected by DNA-binding proteins from attack by endonuclease enzymes, which break down DNA into smaller fragments by cleaving its phosphodiester bonds. The technique does this with gel electrophoresis to find out which parts of the DNA have sites for proteins to attach to them, by comparing DNA which has been in the presence of DNA-binding proteins with DNA that is pure.
(09 Oct 1997)
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footprinting A DNase footprinting assay is a technique from molecular biology that detects DNA-protein interaction using the fact that a protein bound to DNA will often protect that DNA from enzymatic cleavage. The method uses restriction enzymes or other deoxyribonucleases (DNases, for short) to cut the DNA, followed by gel electrophoresis to detect the resulting cleavage pattern. ...
Ãâó: en.wikipedia.org/wiki/Footprinting
footprinting A technique to determine the length of nucleic acid in contact with a protein. While in contact the free DNA is digested. The remaining DNA is then isolated and characterized.
Ãâó: helios.bto.ed.ac.uk/bto/glossary/ef.htm
footprinting Assay used to study DNAbinding proteins.
Ãâó: www.kumc.edu/gec/gloss.html
footprinting is a technique for identifying the site on DNA bound by some protein by virtue of the protection of bonds in this region against attack by nucleases.
Ãâó: www.hgsc.bcm.tmc.edu/docs/HGSC_glossary.html
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