| FPIA | fluorescence polarization immunoassay |
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| FIA | fistula in ano; fluorescent immunoassay; focal immunoassay; Freund incomplete adjuvant |
| FP | false positive; family physician; family planning; family practice; family practitioner; Fanconi pan... |
| PCFIA | particle concentration of fluorescence immunoassay |
| SPIF | solid-phase immunoassay fluorescence |
| FP | FLuorescence polarization |
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| FPIA | Fluorescence Polarisation Immunoassay |
| FIA | Fluorescence immunoassay |
| CP | Cross-polarization |
| DNP | Dynamic nuclear polarization |
| fluorescence polarization immunoassay | Fluoroimmunoassay where detection of the hapten-antibody reaction is based on measurement of the increased polarization of fluorescence-labelled hapten when it is combined with antibody. The assay is very useful for the measurement of small haptenic antigens such as drugs at low concentrations. (12 Dec 1998) |
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| fluorescence polarization | Measurement of the polarization of fluorescent light from solutions or microscopic specimens. It is used to provide information concerning molecular size, shape, and conformation, molecular anisotropy, electronic energy transfer, molecular interaction, including dye and coenzyme binding, and the antigen-antibody reaction. (12 Dec 1998) |
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| fluorescence immunoassay | <technique> A sensitive technique which uses fluorescein, a fluorescent molecule, to measure the antigen or antibody concentration in a solution. (09 Oct 1997) |
| fluorescence polarisation immunoassay | A technique which takes advantage of the increased polarisation (non-random propagation of emission) of fluorescent light emissions when a fluorescent labelled antigen is bound by reagent antibody. The higher the concentration of unlabelled patient antigen present in the test mixture, the less bound fluorescent antigen is present and, consequently, the lower the polarisation of the fluorescent light emission. Standard calibration yields quantitative results. (05 Mar 2000) |
| angle of polarization | <optics> The angle of incidence at which the reflected light is all polarised. (05 Mar 2000) |
| microscopy, polarization | Microscopy using polarised light in which phenomena due to the preferential orientation of optical properties with respect to the vibration plane of the polarised light are made visible and correlated parameters are made measurable. (12 Dec 1998) |
| polarization | 1. The act of polarizing; the state of being polarized, or of having polarity. 2. <optics> A peculiar affection or condition of the rays of light or heat, in consequence of which they exhibit different properties in different directions. If a beam of light, which has been reflected from a plate of unsilvered glass at an angle of about 56 deg, be received upon a second plate of glass similar to the former, and at the same angle of incidence, the light will be readily reflected when the two planes of incidence are parallel to each other, but will not be reflected when the two planes of incidence are perpendicular to each other. The light has, therefore, acquired new properties by reflection from the first plate of glass, and is called polarized light, while the modification which the light has experienced by this reflection is called polarization. The plane in which the beam of light is reflected from the first mirror is called the plane of polarization. The angle of polarization is the angle at which a beam of light must be reflected, in order that the polarization may be the most complete. The term polarization was derived from the theory of emission, and it was conceived that each luminous molecule has two poles analogous to the poles of a magnet; but this view is not now held. According to the undulatory theory, ordinary light is produced by vibrations transverse or perpendicular to the direction of the ray, and distributed as to show no distinction as to any particular direction. But when, by any means, these, vibrations are made to take place in one plane, the light is said to be plane polarized. If only a portion of the vibrations lie in one plane the ray is said to be partially polarized. Light may be polarized by several methods other than by reflection, as by refraction through most crystalline media, or by being transmitted obliquely through several plates of glass with parallel faces. If a beam of polarized light be transmitted through a crystal of quartz in the direction of its axis, the plane of polarization will be changed by an angle proportional to the thickness of the crystal. This phenomenon is called rotatory polarization. A beam of light reflected from a metallic surface, or from glass surfaces under certain peculiar conditions, acquires properties still more complex, its vibrations being no longer rectilinear, but circular, or elliptical. This phenomenon is called circular or elliptical polarization. 3. <physics> An effect produced upon the plates of a voltaic battery, or the electrodes in an electrolytic cell, by the deposition upon them of the gases liberated by the action of the current. It is chiefly due to the hydrogen, and results in an increase of the resistance, and the setting up of an opposing electromotive force, both of which tend materially to weaken the current of the battery, or that passing through the cell. Origin: Cf. F. Polarization. Source: Websters Dictionary (01 Mar 1998) |
| polarization colours | <microscopy> Interference colours produced by anisotropic substances placed between two polarizing elements and examined by transmitted white light. See: Michel-Levy scale of retardation colours. (05 Aug 1998) |
| polarization microscopy | <procedure> Any form of microscopy capable of detecting birefringent objects. Usually performed with a polarizing element below the stage to produce plane polarized light and an analyser that is set to give total extinction of the background and thus to detect any birefringence. (18 Nov 1997) |
| microparticle enzyme immunoassay | A technique in which the solid-phase support consists of very small microparticles in liquid suspension. Specific reagent antibodies are covalently bound to the microparticles. Antigen, if present, is then "sandwiched" between bound antibodies and antigen-specific, enzyme-labelled antibodies. Antigen-antibody complexes are detected and quantitated by analysis of fluorescence from the enzyme-substrate interaction. Acronym: MEIA (05 Mar 2000) |
| solid phase immunoassay | Immunoassay in which the antigen or serum is bound to a solid surface, such as a microplate wall or the sides of a tube, the other reactants being free in solution. (05 Mar 2000) |
| double antibody immunoassay | A method of separating antibody-bound antigen (e.g., insulin) from free antigen by precipitating the former with antibody specific for immunoglobulin. Synonym: double antibody immunoassay, double antibody method. (05 Mar 2000) |
| immunoassay | <investigation> A process that measures and identifies a specific biological substance such as an antigen. (09 Oct 1997) |
| thin-layer immunoassay | A method for detection of antigen-antibody reactions, applicable to detection of either antigen or antibody, based on the fact that either reactant, when added to a polystyrene surface (such as a well in a polystyrene plate) is adsorbed as a thin layer and acts as an immunosorbent capable of binding with the second reactant. (05 Mar 2000) |
| enzyme immunoassay | The general term for an expanding technical arsenal of testing which allows a full range of quantitative analyses for both antigen and antibodies. These tests use colour-changed products of enzyme-substrate interaction (or inhibition) to measure the antigen-antibody reaction. Examples of EIA procedures (EMIT, ELISA, MAC, MEIA) follow. Acronym: EIA (05 Mar 2000) |
| enzyme-multiplied immunoassay technique | A type of immunoassay in which the ligand is labelled with an enzyme, and the enzyme-ligand-antibody complex is enzymatically inactive, allowing quantitation of unlabelled ligand. The test uses antibodies that react only with the particular drug for which the sample is being tested. The antibodies attach themselves to the drug if it is present in the sample. It is not designed to measure amounts of the drug present, only to detect its presence or absence. It is used predominantly, but not exclusively, for the detection of drugs of abuse in the urine. See: competitive binding assay, enzyme-linked immunosorbent assay. (05 Mar 2000) |
Synonyms : Fluorescence Polarization Immunoassays, Fluoroimmunoassay, Polarization, Fluoroimmunoassays, Polarization, Immunoassays, Fluorescence Polarization, Polarization Fluoroimmunoassays, Polarization Immunoassay, Fluorescence
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