| FP | false positive; family physician; family planning; family practice; family practitioner; Fanconi pan... |
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| FPIA | fluorescence polarization immunoassay |
| CIDEP | chemically induced dynamic electron polarization |
| CIDNP | chemically induced dynamic nuclear polarization |
| DEPT | distortionless enhancement by polarization transfer |
| FP | FLuorescence polarization |
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| CP | Cross-polarization |
| DNP | Dynamic nuclear polarization |
| GP | Generalized Polarization |
| photo-CIDNP | Photo-chemically induced dynamic nuclear polarization |
| fluorescence polarization | Measurement of the polarization of fluorescent light from solutions or microscopic specimens. It is used to provide information concerning molecular size, shape, and conformation, molecular anisotropy, electronic energy transfer, molecular interaction, including dye and coenzyme binding, and the antigen-antibody reaction. (12 Dec 1998) |
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| fluorescence polarization immunoassay | Fluoroimmunoassay where detection of the hapten-antibody reaction is based on measurement of the increased polarization of fluorescence-labelled hapten when it is combined with antibody. The assay is very useful for the measurement of small haptenic antigens such as drugs at low concentrations. (12 Dec 1998) |
| angle of polarization | <optics> The angle of incidence at which the reflected light is all polarised. (05 Mar 2000) |
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| microscopy, polarization | Microscopy using polarised light in which phenomena due to the preferential orientation of optical properties with respect to the vibration plane of the polarised light are made visible and correlated parameters are made measurable. (12 Dec 1998) |
| polarization | 1. The act of polarizing; the state of being polarized, or of having polarity. 2. <optics> A peculiar affection or condition of the rays of light or heat, in consequence of which they exhibit different properties in different directions. If a beam of light, which has been reflected from a plate of unsilvered glass at an angle of about 56 deg, be received upon a second plate of glass similar to the former, and at the same angle of incidence, the light will be readily reflected when the two planes of incidence are parallel to each other, but will not be reflected when the two planes of incidence are perpendicular to each other. The light has, therefore, acquired new properties by reflection from the first plate of glass, and is called polarized light, while the modification which the light has experienced by this reflection is called polarization. The plane in which the beam of light is reflected from the first mirror is called the plane of polarization. The angle of polarization is the angle at which a beam of light must be reflected, in order that the polarization may be the most complete. The term polarization was derived from the theory of emission, and it was conceived that each luminous molecule has two poles analogous to the poles of a magnet; but this view is not now held. According to the undulatory theory, ordinary light is produced by vibrations transverse or perpendicular to the direction of the ray, and distributed as to show no distinction as to any particular direction. But when, by any means, these, vibrations are made to take place in one plane, the light is said to be plane polarized. If only a portion of the vibrations lie in one plane the ray is said to be partially polarized. Light may be polarized by several methods other than by reflection, as by refraction through most crystalline media, or by being transmitted obliquely through several plates of glass with parallel faces. If a beam of polarized light be transmitted through a crystal of quartz in the direction of its axis, the plane of polarization will be changed by an angle proportional to the thickness of the crystal. This phenomenon is called rotatory polarization. A beam of light reflected from a metallic surface, or from glass surfaces under certain peculiar conditions, acquires properties still more complex, its vibrations being no longer rectilinear, but circular, or elliptical. This phenomenon is called circular or elliptical polarization. 3. <physics> An effect produced upon the plates of a voltaic battery, or the electrodes in an electrolytic cell, by the deposition upon them of the gases liberated by the action of the current. It is chiefly due to the hydrogen, and results in an increase of the resistance, and the setting up of an opposing electromotive force, both of which tend materially to weaken the current of the battery, or that passing through the cell. Origin: Cf. F. Polarization. Source: Websters Dictionary (01 Mar 1998) |
| polarization colours | <microscopy> Interference colours produced by anisotropic substances placed between two polarizing elements and examined by transmitted white light. See: Michel-Levy scale of retardation colours. (05 Aug 1998) |
| polarization microscopy | <procedure> Any form of microscopy capable of detecting birefringent objects. Usually performed with a polarizing element below the stage to produce plane polarized light and an analyser that is set to give total extinction of the background and thus to detect any birefringence. (18 Nov 1997) |
| ratio imaging fluorescence microscopy | <procedure> A method of measurement of intracellular pH or intracellular calcium levels, using a fluorescent probe molecule (see fura-2), in which the two different excitation wavelengths are used and the emitted light levels compared. If emission at one wavelength is sensitive to the intracellular ion level and emission at the other wavelength is not, then standardisation for intracellular probe concentration, efficiency of light collection, inactivation of probe and thickness of cytoplasm can all be performed automatically. (17 Dec 1997) |
| microscopy, fluorescence | Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilises antibodies that are labelled with fluorescent dye. (12 Dec 1998) |
| spectrometry, fluorescence | Measurement of the intensity and quality of fluorescence. (12 Dec 1998) |
| Eranko's fluorescence stain | <technique> Exposure of frozen sections to formaldehyde which produces a strong yellow-green fluorescence from cells containing norepinephrine. (05 Mar 2000) |
| fluorescence | <chemistry, physics> The emission of one or more photons by a molecule or atom activated by the absorption of a quantum of electro magnetic radiation. Typically the emission, that is of longer wavelength than the excitatory radiation, occurs within 10exp 8 seconds: phosphorescence is a phenomenon with a longer or much longer delay in re radiation. Note that rays, X-rays, UV, visible light and IR radiations may all stimulate fluorescence. (25 Jun 1999) |
| fluorescence-activated cell sorter | <technique> Flow cytometry is an emerging technique which holds great promise for the separation, classification and quantitation of blood cells and antibodies which affect blood cells. Complex computerised instruments are used to pass a monocellular stream of cells, platelets or other microscopic particulate elements through a beam of laser light. The cells are categorised first by size and then computer analysed to sort the mixture of cellular elements into cell type by size. Cells are labelled with fluorescent dye and then passed, in suspending medium, through a narrow dropping nozzle so that each cell is in a small droplet. A laser based detector system is used to excite fluorescence and droplets with positively fluorescent cells are given an electric charge. Charged and uncharged droplets are separated as they fall between charged plates and so collect in different tubes. The machine can be used either as an analytical tool, counting the number of labelled cells in a population or to separate the cells for subsequent growth of the selected population. Further sophistication can be built into the system by using a second laser system at right angles to the first to look at a second fluorescent label or to gauge cell size on the basis of light scatter. The great strength of the system is that it looks at large numbers of individual cells and makes possible the separation of populations with, for example: particular surface properties. Tabulation of counted data in conjunction with size analysis enables determination of relative percentages of each specific cellular subset for which monoclonal antibody conjugates are utilised, even when the size of the cell is identical to other subset species. Flow cytometry is a slightly imprecise but common term for the use of the Fluorescence-activated Cell Sorter (FACS). (01 Dec 1998) |
| fluorescence-activated cell sorting | <technique> A technique for separating and sorting cells marked with a fluorescent label based on how much they fluoresce at a particular wavelength. (12 Jan 1998) |
| fluorescence energy transfer | <technique> Transfer of energy from one fluorochrome to another. The emission wavelength of the fluorochrome excited by the incident light must approximately match the excitation wavelength of the second fluorochrome. If light at the second emission wavelength is detected, it implies that the two fluorochromes were physically within a few nanometres. Used as a technique to probe protein or cell interactions. (25 Jun 1999) |
| fluorescence immunoassay | <technique> A sensitive technique which uses fluorescein, a fluorescent molecule, to measure the antigen or antibody concentration in a solution. (09 Oct 1997) |
| fluorescence in situ hybridization | <molecular biology, technique> A type of in situ hybridization in which target sequences are stained with fluorescent dye so their location and size can be determined using fluorescence microscopy. This staining is sufficiently distinct that the hybridization signal can be seen both in metaphase spreads and in interphase nuclei. Acronym: FISH (25 Jun 1999) |
Synonyms : Anisotropies, Fluorescence, Fluorescence Anisotropies, Fluorescence Polarizations, Polarizations, Fluorescence
Synonyms : Fluorescence Polarization Immunoassays, Fluoroimmunoassay, Polarization, Fluoroimmunoassays, Polarization, Immunoassays, Fluorescence Polarization, Polarization Fluoroimmunoassays, Polarization Immunoassay, Fluorescence
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