| ICC | immunocompetent cells; immunocytochemistry; Indian childhood cirrhosis; intensive coronary care; int... |
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| FIA | fistula in ano; fluorescent immunoassay; focal immunoassay; Freund incomplete adjuvant |
| FPIA | fluorescence polarization immunoassay |
| PCFIA | particle concentration of fluorescence immunoassay |
| SPIF | solid-phase immunoassay fluorescence |
| FCS | Fluorescence Correlation Spectroscopy |
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| FPIA | Fluorescence Polarisation Immunoassay |
| FIA | Fluorescence immunoassay |
| CC | Correlation coefficient |
| COSY | Correlation spectroscopy |
| fluorescence immunoassay | <technique> A sensitive technique which uses fluorescein, a fluorescent molecule, to measure the antigen or antibody concentration in a solution. (09 Oct 1997) |
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| fluorescence polarisation immunoassay | A technique which takes advantage of the increased polarisation (non-random propagation of emission) of fluorescent light emissions when a fluorescent labelled antigen is bound by reagent antibody. The higher the concentration of unlabelled patient antigen present in the test mixture, the less bound fluorescent antigen is present and, consequently, the lower the polarisation of the fluorescent light emission. Standard calibration yields quantitative results. (05 Mar 2000) |
| fluorescence polarization immunoassay | Fluoroimmunoassay where detection of the hapten-antibody reaction is based on measurement of the increased polarization of fluorescence-labelled hapten when it is combined with antibody. The assay is very useful for the measurement of small haptenic antigens such as drugs at low concentrations. (12 Dec 1998) |
| rank-difference correlation | The relationship between paired series of measurements, each ranked according to magnitude, which yields a coefficient known as rho; the value of rho varies from zero (no relationship) to +1.00 (perfect relationship). (05 Mar 2000) |
| correlation | <statistics> most generally, the degree to which one phenomenon or random variable is associated with or can be predicted from another. In statistics, correlation usually refers to the degree to which a linear predictive relationship exists between random variables, as measured by a correlation coefficient. Correlation may be positive, i.e., both variables increase or decrease together or negative or inverse, i.e., one variable increases when the other decreases. (18 Nov 1997) |
| correlation coefficient | A measure of association that indicates the degree to which two variables have a linear relationship; this coefficient, represented by the letter r, can vary between +1 and -1; when r = +1, there is a perfect positive linear relationship in which one variable relates directly with the other; when r = -1, there is a perfect negative linear relationship between the variables. (05 Mar 2000) |
| product-moment correlation | A statistical procedure which yields the correlation coefficient referred to as r (-1.00 to +1.00) and involves the actual values, rather than the ranks (rank order) of the measurements. (05 Mar 2000) |
| microparticle enzyme immunoassay | A technique in which the solid-phase support consists of very small microparticles in liquid suspension. Specific reagent antibodies are covalently bound to the microparticles. Antigen, if present, is then "sandwiched" between bound antibodies and antigen-specific, enzyme-labelled antibodies. Antigen-antibody complexes are detected and quantitated by analysis of fluorescence from the enzyme-substrate interaction. Acronym: MEIA (05 Mar 2000) |
| solid phase immunoassay | Immunoassay in which the antigen or serum is bound to a solid surface, such as a microplate wall or the sides of a tube, the other reactants being free in solution. (05 Mar 2000) |
| double antibody immunoassay | A method of separating antibody-bound antigen (e.g., insulin) from free antigen by precipitating the former with antibody specific for immunoglobulin. Synonym: double antibody immunoassay, double antibody method. (05 Mar 2000) |
| immunoassay | <investigation> A process that measures and identifies a specific biological substance such as an antigen. (09 Oct 1997) |
| thin-layer immunoassay | A method for detection of antigen-antibody reactions, applicable to detection of either antigen or antibody, based on the fact that either reactant, when added to a polystyrene surface (such as a well in a polystyrene plate) is adsorbed as a thin layer and acts as an immunosorbent capable of binding with the second reactant. (05 Mar 2000) |
| enzyme immunoassay | The general term for an expanding technical arsenal of testing which allows a full range of quantitative analyses for both antigen and antibodies. These tests use colour-changed products of enzyme-substrate interaction (or inhibition) to measure the antigen-antibody reaction. Examples of EIA procedures (EMIT, ELISA, MAC, MEIA) follow. Acronym: EIA (05 Mar 2000) |
| enzyme-multiplied immunoassay technique | A type of immunoassay in which the ligand is labelled with an enzyme, and the enzyme-ligand-antibody complex is enzymatically inactive, allowing quantitation of unlabelled ligand. The test uses antibodies that react only with the particular drug for which the sample is being tested. The antibodies attach themselves to the drug if it is present in the sample. It is not designed to measure amounts of the drug present, only to detect its presence or absence. It is used predominantly, but not exclusively, for the detection of drugs of abuse in the urine. See: competitive binding assay, enzyme-linked immunosorbent assay. (05 Mar 2000) |
| ratio imaging fluorescence microscopy | <procedure> A method of measurement of intracellular pH or intracellular calcium levels, using a fluorescent probe molecule (see fura-2), in which the two different excitation wavelengths are used and the emitted light levels compared. If emission at one wavelength is sensitive to the intracellular ion level and emission at the other wavelength is not, then standardisation for intracellular probe concentration, efficiency of light collection, inactivation of probe and thickness of cytoplasm can all be performed automatically. (17 Dec 1997) |
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