| ¿µ¹® | electrophoresis | ÇÑ±Û | Àü±âÀ̵¿ |
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| UPEP | urinary protein electrophoresis; urine protein electrophoresis |
|---|---|
| CIE | Counter(current) Immuno-Electrophoresis; ¿ª¸é¿ª Àü±â ¿µµ¿¹ý |
| PAGE | Poly-Acrylamide Gel Electrophoresis |
| SDS-PAGE | Sodium DodecylSulfate-PolyacrylAmide Gel Electrophoresis |
| 2-DE | 2-Dimensional Electrophoresis |
| ACE | Affinity capillary electrophoresis |
|---|---|
| AGE | Agarose gel electrophoresis |
| BN-PAGE | Blue Native polyacrylamide gel electrophoresis |
| CGE | Capillary Gel Electrophoresis |
| CZE | Capillary Zone Electrophoresis |
| electrophoresis | <technique> Separation of ionic molecules, (principally proteins) by the differential migration through a gel according to the size and ionic charge of the molecules in an electrical field. High resolution techniques normally use a gel support for the fluid phase. Examples of gels used are starch, acrylamide, agarose or mixtures of acrylamide and agarose. Frictional resistance produced by the support causes size, rather than charge alone, to become the major determinant of separation. Smaller molecules with a more negative charge will travel faster and further through the gel toward the anode of an electrophoretic cell when high voltage is applied. Similar molecules will group on the gel. They may be visualised by staining and quantitated, in relative terms, using densitometers which continuously monitor the photometric density of the resulting stain. The electrolyte may be continuous (a single buffer) or discontinuous, where a sample is stacked by means of a buffer discontinuity, before it enters the running gel/ running buffer. The gel may be a single concentration or gradient in which pore size decreases with migration distance. In SDS gel electrophoresis of proteins or electrophoresis of polynucleotides, mobility depends primarily on size and is used to determined molecular weight. In pulse field electrophoresis, two fields are applied alternately at right angles to each other to minimise diffusion mediated spread of large linear polymers. See: electrofocussing, pulse field electrophoresis (01 Dec 1998) |
|---|---|
| electrophoresis, agar gel | Electrophoresis in which agar or agarose gel is used as the diffusion medium. (12 Dec 1998) |
| electrophoresis, capillary | A highly-sensitive (in the picomolar range, which is 10,000-fold more sensitive than conventional electrophoresis) and efficient technique that allows separation of proteins, nucleic acids, and carbohydrates. (12 Dec 1998) |
| electrophoresis, cellulose acetate | Electrophoresis in which cellulose acetate is the diffusion medium. (12 Dec 1998) |
| electrophoresis, disc | Electrophoresis in which discontinuities in both the voltage and pH gradients are introduced by using buffers of different composition and pH in the different parts of the gel column. The term 'disc' was originally used as an abbreviation for 'discontinuous' referring to the buffers employed, and does not have anything to do with the shape of the separated zones. (12 Dec 1998) |
| electrophoresis, gel, pulsed-field | Electrophoresis in which the direction of the electric field is changed periodically. This technique is similar to other electrophoretic methods normally used to separate double-stranded DNA molecules ranging in size up to tens of thousands of base-pairs. However, by alternating the electric field direction one is able to separate DNA molecules up to several million base-pairs in length. (12 Dec 1998) |
| electrophoresis, gel, two-dimensional | Electrophoresis in which a second perpendicular electrophoretic transport is performed on the separate components resulting from the first electrophoresis. This technique is usually performed on polyacrylamide gels. (12 Dec 1998) |
| electrophoresis, paper | Electrophoresis in which paper is used as the diffusion medium. This technique is confined almost entirely to separations of small molecules such as amino acids, peptides, and nucleotides, and relatively high voltages are nearly always used. (12 Dec 1998) |
| electrophoresis, polyacrylamide gel | Electrophoresis in which a polyacrylamide gel is used as the diffusion medium. (12 Dec 1998) |
| electrophoresis, starch gel | Electrophoresis in which a starch gel (a mixture of amylose and amylopectin) is used as the diffusion medium. (12 Dec 1998) |
| agarose gel electrophoresis | <procedure> A type of electrophoresis that uses a matrix of highly purified agar to separate large nucleotides in size. (06 May 1997) |
|---|---|
| blood protein electrophoresis | Electrophoresis applied to blood proteins. (12 Dec 1998) |
| capillary electrophoresis | A technique for separating compounds, a sample of a compound to beseparated is placed in a capillary tube, which is then subjected to ahigh voltage current that separates its chemical components. (09 Oct 1997) |
| capillary zone electrophoresis | A method for separating molecules extremely rapidly based on their electrophoretic mobility. (05 Mar 2000) |
| gamaglobulin electrophoresis | <investigation> A test that detects and measures the various immunoglobulins in the blood. In the normal assay no monoclonal antibodies are detected. In multiple myeloma and chronic lymphocytic leukaemia a single clone of lymphocytes can produce one type of immunoglobulin that is detected in the electrophoresis as monoclonal (made by one cell clone). (27 Sep 1997) |
| gel electrophoresis | <molecular biology> Electrophoresis using a gel supporting phase. Usually applied to systems where the gel is based on polyacrylamide. See: electrophoresis. (05 May 1997) |
| carrier electrophoresis | Electrophoresis done on a carrier (such as paper, polyacrylamide gel, etc.). (05 Mar 2000) |
| cell electrophoresis | <technique> A method for estimating the surface charge of a cell by looking at its rate of movement in an electrical field. Almost all eukaryotic cells have a net negative surface charge. Measurement is complicated by the streaming potential at the wall of the chamber itself and by the fact that the cell is surrounded by a layer of fluid (see double layer). The electrical potential measured (the zeta potential) is actually some distance away from the plasma membrane. One of the more useful modifications is to systematically vary the pH of the suspension fluid to determine the pK of the charged groups responsible (mostly carboxyl groups of sialic acid). (26 Mar 1998) |
| multi locus enzyme electrophoresis | Inhibition of translation of the transcript of a transposase gene by a multicopy plasmid with suitable inhibitory gene. The plasmid inhibits transposition events in the host bacterium. (18 Nov 1997) |
| polyacrylamide gel electrophoresis | Analytical and separative technique in which molecules, particularly proteins, are separated by their different electrophoretic mobilities in a hydrated gel. The gel suppresses convective mixing of the fluid phase through which the electrophoresis takes place and contributes molecular sieving. Commonly carried out in the presence of the anionic detergent sodium dodecylsulphate (SDS). SDS denatures proteins so that noncovalently associating sub unit polypeptides migrate independently and by binding to the proteins confers a net negative charge roughly proportional to the chain weight. See: SDS PAGE. (21 Jun 1999) |
| haemoglobin electrophoresis | <investigation> A special diagnostic procedure which identifies abnormal haemoglobin proteins by the way they migrate in an electric field (electrophoresis). The electric field is used to separate haemoglobin proteins from each other and allow the identification of different components. This can be used to diagnose thalassaemia, sickle cell disease and haemoglobin C disease. (18 Nov 1997) |
| pulsed-field gel electrophoresis | Gel electrophoresis in which, after electrophoretic migration has begun, the current is briefly stopped and reapplied in a different orientation; allows for the purification of long DNA molecules. Synonym: pulsed-field gel electrophoresis. (05 Mar 2000) |
| pulse field electrophoresis | <investigation> A method used for high resolution electrophoretic separation of very large (megabase) fragments of DNA. Electric fields 100 |
| pulse-field gel electrophoresis | Gel electrophoresis in which, after electrophoretic migration has begun, the current is briefly stopped and reapplied in a different orientation; allows for the purification of long DNA molecules. Synonym: pulsed-field gel electrophoresis. (05 Mar 2000) |
| serum immunoglobulin electrophoresis | A test that detects and measures the various immunoglobulins in the blood. In the normal assay no monoclonal antibodies are detected. In multiple myeloma and chronic lymphocytic leukaemia a single clone of lymphocytes can produce one type of immunoglobulin that is detected in the electrophoresis as monoclonal (made by one cell clone). (27 Sep 1997) |
Synonyms : Electrophoreses, Isotachophoreses
Synonyms : Agar Gel Electrophoresis, Agarose Gel Electrophoresis, Gel Electrophoresis, Agar, Gel Electrophoresis, Agarose
Synonyms : Capillary Zone Electrophoresis, Capillary Electrophoreses, Capillary Electrophoresis, Capillary Zone Electrophoreses, Electrophoreses, Capillary, Electrophoreses, Capillary Zone, Electrophoresis, Capillary Zone, Zone Electrophoreses, Capillary
Synonyms : Cellulose Acetate Electrophoreses, Cellulose Acetate Electrophoresis, Electrophoreses, Cellulose Acetate
Synonyms : Electrophoresis, Disk, Disc Electrophoresis, Disk Electrophoresis
| electrophoresis |
the motion of charged particles in a colloid under the influence of an electric field; particles with a positive charge go to the cathode and negative to the anode
Ãâó: wordnet.princeton.edu/perl/webwn
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|---|---|
| electrophoresis |
A technique that separates charged molecules - such as DNA, RNA or protein - on the basis of relative migration in an appropriate matrix (such as agarose gel or polyacrylamide gel) subjected to an electric field. See agarose gel electrophoresis; polyacrylamide gel electrophoresis (PAGE); pulsed-field gel electrophoresis (PFGE).
Ãâó: www.fao.org/docrep/003/X3910E/X3910E08.htm
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| electrophoresis |
A procedure by means of which the members of a heterogenous population of charged particles can be separated by virtue of their dissimilar migration characteristics in an electric field. (16)
Ãâó: ppathw3.cals.cornell.edu/glossary/Defs_E.htm
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| electrophoresis |
a technique which uses the differential mobility of differently charged molecules in an electric field to separate them within a stationary gel.
Ãâó: www.mycolog.com/GLOSSARY.htm
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| electrophoresis |
A technique for separating mixtures of organic molecules based on their different rates of travel in electric fields.
Ãâó: biology.usgs.gov/s+t/SNT/noframe/zy198.htm
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| electrophoresis | the motion of charged particles in a colloid under the influence of an electric field |
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