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  • JrId: 3579
    JournalTitle: Electrophoresis.
    MedAbbr: Electrophoresis
    ISSN: 0173-0835
    ESSN:
    IsoAbbr: Electrophoresis
    NlmId: 8204476
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electrophoresis <technique> Separation of ionic molecules, (principally proteins) by the differential migration through a gel according to the size and ionic charge of the molecules in an electrical field. High resolution techniques normally use a gel support for the fluid phase.
Examples of gels used are starch, acrylamide, agarose or mixtures of acrylamide and agarose. Frictional resistance produced by the support causes size, rather than charge alone, to become the major determinant of separation.
Smaller molecules with a more negative charge will travel faster and further through the gel toward the anode of an electrophoretic cell when high voltage is applied. Similar molecules will group on the gel. They may be visualised by staining and quantitated, in relative terms, using densitometers which continuously monitor the photometric density of the resulting stain.
The electrolyte may be continuous (a single buffer) or discontinuous, where a sample is stacked by means of a buffer discontinuity, before it enters the running gel/ running buffer. The gel may be a single concentration or gradient in which pore size decreases with migration distance.
In SDS gel electrophoresis of proteins or electrophoresis of polynucleotides, mobility depends primarily on size and is used to determined molecular weight. In pulse field electrophoresis, two fields are applied alternately at right angles to each other to minimise diffusion mediated spread of large linear polymers.
See: electrofocussing, pulse field electrophoresis
(01 Dec 1998)
electrophoresis, agar gel Electrophoresis in which agar or agarose gel is used as the diffusion medium.
(12 Dec 1998)
electrophoresis, capillary A highly-sensitive (in the picomolar range, which is 10,000-fold more sensitive than conventional electrophoresis) and efficient technique that allows separation of proteins, nucleic acids, and carbohydrates.
(12 Dec 1998)
electrophoresis, cellulose acetate Electrophoresis in which cellulose acetate is the diffusion medium.
(12 Dec 1998)
electrophoresis, disc Electrophoresis in which discontinuities in both the voltage and pH gradients are introduced by using buffers of different composition and pH in the different parts of the gel column. The term 'disc' was originally used as an abbreviation for 'discontinuous' referring to the buffers employed, and does not have anything to do with the shape of the separated zones.
(12 Dec 1998)
electrophoresis, gel, pulsed-field Electrophoresis in which the direction of the electric field is changed periodically. This technique is similar to other electrophoretic methods normally used to separate double-stranded DNA molecules ranging in size up to tens of thousands of base-pairs. However, by alternating the electric field direction one is able to separate DNA molecules up to several million base-pairs in length.
(12 Dec 1998)
electrophoresis, gel, two-dimensional Electrophoresis in which a second perpendicular electrophoretic transport is performed on the separate components resulting from the first electrophoresis. This technique is usually performed on polyacrylamide gels.
(12 Dec 1998)
electrophoresis, paper Electrophoresis in which paper is used as the diffusion medium. This technique is confined almost entirely to separations of small molecules such as amino acids, peptides, and nucleotides, and relatively high voltages are nearly always used.
(12 Dec 1998)
electrophoresis, polyacrylamide gel Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
(12 Dec 1998)
electrophoresis, starch gel Electrophoresis in which a starch gel (a mixture of amylose and amylopectin) is used as the diffusion medium.
(12 Dec 1998)
electrophoretic Relating to electrophoresis, as an electrophoretic separation.
Synonym: ionophoretic.
(05 Mar 2000)
electrophoretogram <chemistry> The densitometric or colourimetric pattern obtained from filter paper or similar porous strips on which substances have been separated by electrophoresis; may also refer to the strips themselves.
Synonym: electrophoretogram, ionogram, ionopherogram.
(05 Mar 2000)
MeSH(Medical Subject Headings) ¸ÂÃã °Ë»ö (http://www.nlm.nih.gov) °á°ú : 5 ÆäÀÌÁö: 1
  • Electrophoresis - »õâ An electrochemical process in which macromolecules or colloidal particles with a net electric charge migrate in a solution under the influence of an electric current.
    Synonyms : Electrophoreses, Isotachophoreses
  • Electrophoresis, Agar Gel - »õâ Electrophoresis in which agar or agarose gel is used as the diffusion medium.
    Synonyms : Agar Gel Electrophoresis, Agarose Gel Electrophoresis, Gel Electrophoresis, Agar, Gel Electrophoresis, Agarose
  • Electrophoresis, Capillary - »õâ A highly-sensitive (in the picomolar range, which is 10,000-fold more sensitive than conventional electrophoresis) and efficient technique that allows separation of PROTEINS; NUCLEIC ACIDS; and CARBOHYDRATES. (Segen, Dictionary of Modern Medicine, 1992)
    Synonyms : Capillary Zone Electrophoresis, Capillary Electrophoreses, Capillary Electrophoresis, Capillary Zone Electrophoreses, Electrophoreses, Capillary, Electrophoreses, Capillary Zone, Electrophoresis, Capillary Zone, Zone Electrophoreses, Capillary
  • Electrophoresis, Cellulose Acetate - »õâ Electrophoresis in which cellulose acetate is the diffusion medium.
    Synonyms : Cellulose Acetate Electrophoreses, Cellulose Acetate Electrophoresis, Electrophoreses, Cellulose Acetate
  • Electrophoresis, Disc - »õâ Electrophoresis in which discontinuities in both the voltage and pH gradients are introduced by using buffers of different composition and pH in the different parts of the gel column. The term 'disc' was originally used as an abbreviation for 'discontinuous' referring to the buffers employed, and does not have anything to do with the shape of the separated zones.
    Synonyms : Electrophoresis, Disk, Disc Electrophoresis, Disk Electrophoresis
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electrophoretic of or relating to electrophoresis
Ãâó: wordnet.princeton.edu/perl/webwn
electrophoresis the motion of charged particles in a colloid under the influence of an electric field; particles with a positive charge go to the cathode and negative to the anode
Ãâó: wordnet.princeton.edu/perl/webwn
electrophoresis A technique that separates charged molecules - such as DNA, RNA or protein - on the basis of relative migration in an appropriate matrix (such as agarose gel or polyacrylamide gel) subjected to an electric field. See agarose gel electrophoresis; polyacrylamide gel electrophoresis (PAGE); pulsed-field gel electrophoresis (PFGE).
Ãâó: www.fao.org/docrep/003/X3910E/X3910E08.htm
electrophoresis A procedure by means of which the members of a heterogenous population of charged particles can be separated by virtue of their dissimilar migration characteristics in an electric field. (16)
Ãâó: ppathw3.cals.cornell.edu/glossary/Defs_E.htm
electrophoresis a technique which uses the differential mobility of differently charged molecules in an electric field to separate them within a stationary gel.
Ãâó: www.mycolog.com/GLOSSARY.htm
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electrophore the motion of charged particles in a colloid under the influence of an electric field
electrophore of or relating to electrophoresis
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