| DIB | diagnostic interview for borderlines; difficulty in breathing; disability insurance benefits; dot im... |
|---|---|
| DOT | date of transfer; Dictionary of Occupational Titles |
| MDFD | map-dot-fingerprint dystrophy |
| MEWD, MEWDS | multiple evanescent white dot [syndrome] |
| RIBA | Recombinant Immuno-Blot Assay |
| Dot-ELISA | Dot Enzyme-Linked Immunosorbent Assay |
|---|---|
| EITB | Enzyme Linked Immunoelectro Transfer Blot |
| RIBA | Recombinant Immuno Blot Assay |
| SB | Southern Blot |
| SBH | Southern blot hybridisation |
| dot blot | <molecular biology, procedure> Method for detecting a specific protein or message. A spot of solution is dotted onto nitrocellulose paper, a specific antibody or probe is allowed to bind and the presence of bound antibody/probe then shown by using a peroxidase coupled second antibody, as in Western blotting or by other visualisation methods. (18 Nov 1997) |
|---|---|
| black-dot ringworm | Tinea capitis due most commonly to Trichophyton tonsurans or T. Violaceum. (05 Mar 2000) |
| blue dot sign | <clinical sign> A blue or black spot visible beneath the skin on the cranial aspect of testis or epididymis. This is a torsed testicular appendage and is usually quite tender. (05 Mar 2000) |
| map-dot-fingerprint dystrophy | Fingerprint dystrophy accompanied by map-like patterns and microcystic epithelial inclusions. (05 Mar 2000) |
| dot | A small spot. (05 Mar 2000) |
| cell hybridization | Fusion of two or more dissimilar cells, leading to formation of a synkaryon. (05 Mar 2000) |
| cross hybridization | Annealing of a DNA probe to an imperfectly matching DNA molecule. (05 Mar 2000) |
| protein hybridization | The formation of a protein consisting of two or more polypeptide chains from separate and different polypeptide chains. (12 Dec 1998) |
| hybridization | <molecular biology> The process of joining two complementary strands of DNA or one each of DNA and RNA to form a double-stranded molecule. Technique in which single stranded nucleic acids are allowed to interact so that complexes or hybrids, are formed by molecules with sufficiently similar, complementary sequences. By this means the degree of sequence identity can be assessed and specific sequences detected. The hybridisation can be carried out in solution or with one component immobilised on a gel or, most commonly, nitrocellulose paper. Hybrids are detected by various means: visualisation in the electron microscope, by radioactively labelling one component and removing noncomplexed DNA or by washing or digestion with an enzyme that attacks single stranded nucleic acids and finally estimating the radioactivity bound. Hybridisations are done in all combinations: DNA DNA (DNA can be rendered single stranded by heat denaturation), DNA RNA or RNA RNA. In situ hybridisations involve hybridising a labelled nucleic acid (often labelled with a fluorescent dye) to suitably prepared cells or histological sections. This is used particularly to look for specific transcription or localisation of genes to specific chromosomes (FISH analysis). <zoology> The mating of individuals from different species or sub-species. (13 Oct 1997) |
| somatic cell hybridization | Production of a heterokaryon. (05 Mar 2000) |
| nucleic acid hybridization | Widely used technique which exploits the ability of complementary sequences in single-stranded dnas or rnas to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (kendrew, encyclopedia of molecular biology, 1994, p503; dorlands, 28th ed, p781) (12 Dec 1998) |
| DNA hybridization | A technique used to determine the relatedness of microorganisms by the speed and efficiency of the reassociation of single-stranded DNA to form double-stranded DNA when one of the strands originates from one organism and the other strand from another organism; occurs when the base sequences are complementary or nearly so. (05 Mar 2000) |
| in situ hybridization | <molecular biology> The use of a DNA or RNA probe to detect the complementary DNA sequence. (12 Dec 1998) |
| in situ nucleic acid hybridization | <molecular biology> The use of a DNA or RNA probe to detect the complementary DNA sequence. (12 Dec 1998) |
| overlap hybridization | A procedure to find and sequence a gene whose approximate position in a chromosome is known by classical genetic linkage studies. Starting with the known sequence of a gene shown by classical genetics to be near to the novel gene, new clones are picked from a genomic library by hybridisation with a short probe generated from the appropriate end of the known sequence. The new clones are then sequenced, new probes generated and the process repeated until the gene of interest is reached. (18 Nov 1997) |
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