| CSLM | confocal scanning microscopy |
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| DCO | Diploma of the College of Optics |
| opt | best [Lat. optimus]; optics, optician |
| CLSM | Confocal Laser Scanning Microscope |
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| CMTF | Confocal Microscopy Through Focusing |
| CSLM | Confocal Scanning Laser Microscope |
| LSCM | Laser Scanning Confocal Microscope |
| TSCM | Tandem Scanning Confocal Microscopy |
| confocal optics | <microscopy> A (microscope) optical system in which the condenser and objective lenses both focus onto one single point in the specimen. Generally, the image of a pinhole source is focused onto a point in the specimen, and that point is focused by the objective lens onto a point detector or through a mask with a pinhole aperture. With confocal optics, the Abbe limit of resolution can be exceeded since only a limited region of the specimen is viewed at any onetime. (05 Aug 1998) |
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| rectified optics | <microscopy> Microscope lens system correcting the rotation of polarized light that takes place at high-incidence-angle interfaces between the polarizer and analyser. Rectification provides high extinction for polarized-light and Differential Interference Contrast microscopy at high numerical apertures, thus permitting bifringence or phase retardation combined with high in g low phase. (05 Aug 1998) |
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| optics | That branch of physical science which treats of the nature and properties of light, the laws of its modification by opaque and transparent bodies, and the phenomena of vision. Origin: Cf. F. Optique, L. Optice, Gr. (sc). See Optic. Source: Websters Dictionary (01 Mar 1998) |
| electron optics | <study> The science that deals with propagation electrons, as light optics deals with that of light and its phenomena. Eye lens (see lens, eye). (05 Aug 1998) |
| microscopy, confocal | A light microscopic technique in which only a small spot is illuminated and observed at a time. An image is constructed through point-by-point scanning of the field in this manner. Light sources may be conventional or laser, and fluorescence or transmitted observations are possible. (12 Dec 1998) |
| confocal | See: confocal microscope. (05 Mar 2000) |
| confocal microscope | <instrument> A microscope that allows the observer to visualise objects in a single plane of focus, thereby creating a sharper image (usually the objects are fluorescent molecules); a refinement of this microscope uses optical sectioning and a computer to record serial sections. This permits three-dimensional reconstruction. (05 Mar 2000) |
| confocal microscopy | <procedure> A system of (usually) epifluorescence light microscopy in which a fine laser beam of light is scanned over the object through the objective lens. The technique is particularly good at rejecting light from outside the plane of focus and so produces higher effective resolution than is normally achieved. (18 Nov 1997) |
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