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CSLM confocal scanning microscopy
CEM computerized electroencephalographic map; conventional transmission electron microscope
EM early memory; ejection murmur; electromagnetic; electron micrograph; electron microscopy, electron m...
E/M electron microscope, electron microscopy; evaluation and management
ESM ejection systolic murmur; endoscopic specular microscope; ethosuximide
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CLSM Confocal Laser Scanning Microscope
CSLM Confocal Scanning Laser Microscope
LSCM Laser Scanning Confocal Microscope
CMTF Confocal Microscopy Through Focusing
TSCM Tandem Scanning Confocal Microscopy
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confocal microscope <instrument> A microscope that allows the observer to visualise objects in a single plane of focus, thereby creating a sharper image (usually the objects are fluorescent molecules); a refinement of this microscope uses optical sectioning and a computer to record serial sections. This permits three-dimensional reconstruction.
(05 Mar 2000)
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microscopy, confocal A light microscopic technique in which only a small spot is illuminated and observed at a time. An image is constructed through point-by-point scanning of the field in this manner. Light sources may be conventional or laser, and fluorescence or transmitted observations are possible.
(12 Dec 1998)
confocal See: confocal microscope.
(05 Mar 2000)
confocal microscopy <procedure> A system of (usually) epifluorescence light microscopy in which a fine laser beam of light is scanned over the object through the objective lens. The technique is particularly good at rejecting light from outside the plane of focus and so produces higher effective resolution than is normally achieved.
(18 Nov 1997)
confocal optics <microscopy> A (microscope) optical system in which the condenser and objective lenses both focus onto one single point in the specimen. Generally, the image of a pinhole source is focused onto a point in the specimen, and that point is focused by the objective lens onto a point detector or through a mask with a pinhole aperture. With confocal optics, the Abbe limit of resolution can be exceeded since only a limited region of the specimen is viewed at any onetime.
(05 Aug 1998)
binocular microscope <instrument, microscopy> A microscope fitted with double eyepieces for vision with both eyes. The purpose in dividing the same image from a single objective of the usual compound micro-scope is to reduce eyestrain and muscular fatigue which may result from monocular, high-power microscopy.
The purpose in obtaining a different image for each of two oculars is to provide stereoscopy by means of two different angles of view. There are two kinds of stereoscopic microscopes: binobjective (Greenough) older type and monobjective (common main objective) newer type. (See stereo microscope, Greenough microscope, etc.)
(05 Aug 1998)
Rheinberg microscope <instrument> A modified form of dark-field microscope in which the central opaque stop in the condenser is replaced by a coloured filter, producing a background of contrasting colour against which the specimen is illuminated.
(05 Mar 2000)
Greenough microscope <instrument, microscopy> One of two kinds of stereomicroscopes with two separate compound microscopes, one for each eye, focused on the same object. The other kind has a common main objective.
See: binocular microscope.
(05 Aug 1998)
phase-contrast microscope <instrument> A specially constructed microscope that has a special condenser and objective containing a phase-shifting ring whereby small differences in index of refraction are made visible as intensity or contrast differences in the image; particularly useful for examining structural details in transparent specimens such as living or unstained cells and tissues.
(05 Mar 2000)
microscope <instrument> A piece of laboratory equipment that is used to magnify small things that are too small to be seen by the naked eye, or too small for the details to be seen by the naked eye, so that their finer details can be seen and studied.
Examples are the light (or optical) microscope, electron microscope, X-ray microscope, and acoustic microscope.
(09 Oct 1997)
microscope, compound A microscope that consists of two microscopes in series, the first serving as the ocular lens (close to the eye) and the second serving as the objective lens (close to the object to be viewed). Credit for creating the compound microscope goes usually to the dutch spectaclemakers hans and zacharias janssen who in 1590 invented an instrument that could be used as either a microscope or telescope. The compound microscope evolved into the dominant type of optical microscope today.
(12 Dec 1998)
microscope, electron <microscopy> An electron-optical device which produces a magnified image of an object. Detail may be revealed by virtue of selective transmission, reflection, or emission of electrons by the object.
(05 Aug 1998)
microscope, field emission <microscopy> An image-forming device in which a strong electrostatic field causes cold emission of electrons from a sharply rounded point or from a specimen that has been placed on that point. The electrons are accelerated to a phosphorescent screen, or photographic film, giving a visible picture of the variation of emission over the specimen surface.
(05 Aug 1998)
microscope, fluorescent A microscope equipped to examine material that fluoresces under ultraviolet (uv) light.
(12 Dec 1998)
microscope, Greenough <microscopy> A stereoscopic microscope with paired objectives, prisms, and eyepieces invented by H. Greenough. The name is sometimes incorrectly used for any stereoscopic microscope with paired objectives showing erect images.
(05 Aug 1998)
microscope mirror <microscopy> Usually plane on one side and concave on the other. The flat side is generally used unless the objective is of very low power and there is no condenser. The mirror should be so mounted that the concave side can be focused on the specimen.
(05 Aug 1998)
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