| HPLC | high-performance liquid chromatography; high-power liquid chromatography; high-pressure liquid chrom... |
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| CCCC | centrifugal countercurrent chromatography |
| FGC | fibrinogen gel chromatography |
| FPLC | fast protein liquid chromatography |
| GC | ganglion cell; gas chromatography; general circulation; general closure; general condition; generali... |
| RP-HPLC | chromatography and reversed-phase high-performance liquid chromatography |
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| AC | Affinity chromatography |
| CGC | Capillary Gas Chromatography |
| CPC | Centrifugal partition chromatography |
| CCC | Counter-current chromatography |
| chromatography | <investigation> Techniques for separating molecules based on differential absorption and elution. Term for separation methods involving flow of a fluid carrier over a nonmobile absorbing phase. (18 Nov 1997) |
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| chromatography paper | Used in paper chromatography. Synonym: high quality filter paper. Congo red paper, paper impregnated with Congo red; used as a pH indicator, changing from blue-violet at 3.0 to red at 5.0. Filter paper, an unsized paper used in pharmacy and chemistry for filtering solutions; many varieties are used for paper chromatography. (05 Mar 2000) |
| chromatography, affinity | A chromatographic technique that utilises the ability of biological molecules to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (12 Dec 1998) |
| chromatography, agarose | A method of gel filtration chromatography using agarose, the non-ionic component of agar, for the separation of compounds with molecular weights up to several million. (12 Dec 1998) |
| chromatography, deae-cellulose | A type of ion exchange chromatography using diethylaminoethyl cellulose (deae-cellulose) as a positively charged resin. (12 Dec 1998) |
| chromatography, gas | Fractionation of a vaporised sample as a consequence of partition between a mobile gaseous phase and a stationary phase held in a column. Two types are gas-solid chromatography, where the fixed phase is a solid, and gas-liquid, in which the stationary phase is a nonvolatile liquid supported on an inert solid matrix. (12 Dec 1998) |
| chromatography, gel | Chromatography on non-ionic gels without regard to the mechanism of solute discrimination. (12 Dec 1998) |
| chromatography, high pressure liquid | Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. (12 Dec 1998) |
| chromatography, ion exchange | Separation technique in which the stationary phase consists of ion exchange resins. The resins contain loosely held small ions that easily exchange places with other small ions of like charge present in solutions washed over the resins. (12 Dec 1998) |
| chromatography, liquid | Chromatographic techniques in which the mobile phase is a liquid. (12 Dec 1998) |
| chromatography, thin layer | Chromatography on thin layers of adsorbents rather than in columns. The adsorbent can be alumina, silica gel, silicates, charcoals, or cellulose. (12 Dec 1998) |
| absorption chromatography | <investigation> Techniques for separating molecules based on differential absorption and elution. Term for separation methods involving flow of a fluid carrier over a nonmobile absorbing phase. (18 Nov 1997) |
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| adsorption chromatography | Chromatography in which separation of substances is achieved by the difference in degree of adsorption of the compounds to a stationary phase. (05 Mar 2000) |
| affinity chromatography | <investigation> A technique of analytical chemistry used to separate and purify a biological molecule from a mixture, based on the attraction of the molecule of interest to a particular ligand which has been previously attached to a solid, inert substance. The mixture is passed through a column containing the ligand attached to the stationary substance, so that the molecule of interest stays within the column while the rest of the mixture continues through to the end. Then, a different chemical is flushed through the column to detach the molecule from the ligand and bring it out separately from the rest of the mixture. (09 Feb 1998) |
| gas chromatography | <technique> A chromatographic technique (a type of column chromatography) in which the stationary phase is solid while the mobile phase is gaseous samples. The gaseous samples are separated based on their different adsorption ability to the solid phase. (09 Oct 1997) |
| gas-liquid chromatography | <technique> A chemistry lab technique, a type of column chromatography, used to separate the components of a mixed substance. The substance is held stationary by an inert solid coated with an inert liquid which is not likely to evaporate (i.e. Is nonvolatile), while a gas (called an eluant) flows past it bringing out the components one at a time. (09 Oct 1997) |
| paper chromatography | <technique> Separation method in which filter paper is used as the support. A type of chromatography in which the stationary phase is a sheet of special-grade filter paper. It is in all other aspects similar to thin-layer chromatography. Not a very sensitive method, but historically important as one of the first methods available for separating natural compounds. (07 Mar 2000) |
| gel exclusion chromatography | <investigation> A lab technique, a type of column chromatography, used to separate the components of a mixture by molecular size and to collect the molecules which are larger than a certain size. It is similar to gel filtration, small molecules are slowed or trapped by the pores in the gel beads filling the column, while large molecules, too large to fit into the pores, slide past the beads and get to the bottom of the column first. at this point, the large molecules are collected. Gel exclusion refers to the maximum size of molecule which will fit into the gel bead pores, and this lab technique is used to collect the molecules in the mixture which are larger than, or excluded from, the pores. (09 Oct 1997) |
| gel filtration chromatography | See: gel filtration. (05 Mar 2000) |
| partition chromatography | The separation of similar substances by repeated divisions between two immiscible liquids, so that the substances, in effect, cross the partition between the liquids in opposite directions; where one of the liquids is bound as a film on filter paper, the process is termed paper partition chromatography or paper chromatography. (05 Mar 2000) |
| reversed phase chromatography | A form of partitionary chromatography in which the stationary phase is more polar than the mobile phase. (05 Mar 2000) |
| column chromatography | A form of partition, adsorption, ion exchange, or affinity chromatography in which one phase is liquid (aqueous) flowing down a column packed with the second phase, a solid; the dissolved substances form a partition between the solid and liquid phases depending on the chemical and physical conditions of each phase; the more strongly adsorbed solutes reach the bottom of the column later than the less strongly adsorbed ones. (05 Mar 2000) |
| high-performance liquid chromatography | <investigation> A lab technique, a type of column chromatography, which uses a combination of several separation techniques to separate substances at higher resolution. Extremely sharp peaks on the elution profile can be produced with high-performance liquid chromatography (HPLC). (09 Oct 1997) |
| high-pressure liquid chromatography | <investigation> A lab technique, a type of column chromatography, which uses a combination of several separation techniques to separate substances at higher resolution. Extremely sharp peaks on the elution profile can be produced with high-performance liquid chromatography (HPLC). (09 Oct 1997) |
| ion exchange chromatography | <procedure> Separation of molecules by absorption and desorption from charged polymers. An important technique for protein purification. For small molecules the support is usually polystyrene, but for macromolecules, cellulose, acrylamide or agarose supports give less non-specific absorption and denaturation. Typical charged residues are CM carboxymethyl) or DEAE (diethylaminoethyl). (27 Oct 1998) |
| thin layer chromatography | <technique> Chromatography using a thin layer of powdered medium on an inert sheet to support the stationary phase. Faster than paper chromatography, gives higher resolution and requires smaller samples. (18 Nov 1997) |
Synonyms : Chromatographies
Synonyms : Affinity Chromatography, Bioaffinity Chromatography
Synonyms : Agarose Chromatography, Sepharose Chromatography, Agarose Chromatographies, Chromatographies, Agarose, Chromatographies, Sepharose, Sepharose Chromatographies
Synonyms : Chromatography, DEAE Cellulose, DEAE Cellulose Chromatography
Synonyms : Gas Chromatography, Chromatographies, Gas, Chromatographies, Gas-Liquid, Chromatography, Gas Liquid, Gas Chromatographies, Gas-Liquid Chromatographies, Gas-Liquid Chromatography
| chromatography |
any of a diverse group of techniques used to separate mixtures of substances based on differences in the relative affinities of the substances for two different media, one (the mobile phase) a moving fluid and the other (the stationary phase or sorbent) a porous solid or gel or a liquid coated on a solid support; the speed at which each substance is carried along by the mobile phase depends on its solubility (in a liquid mobile phase) or vapor pressure (in a gas mobile phase) and on its affinity for the sorbent.
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| chromatography | a process used for separating mixtures by virtue of differences in absorbency |
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| chromatography | a vertical glass tube used in column chromatography |
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