| 영문 | Gram stain | 한글 | 그람염색 |
|---|---|---|---|
| 설명 | 미생물을 염색하는 방법 중의 하나. 1884년 덴마크의 의사 H.C.J. 그람(1853~ 1938)이 고안한 특수 염색법이다. 이 염색법을 사용하면 미생물은 양성균과 음성균의 2무리로 크게 나뉜다. 염색법은 균을 유리면에 바르고 가열 고정한다. 젠시안바이오렛, 크리스탈 바이오렛, 메틸바이오렛 등 파라로즈 아닐린계 색소의 어느 하나를 사용하여 우선 염색하고 이어서 루골액을 작용시킨 뒤, 에탄올 또는 아세톤으로 탈색한다. 이때 탈색되지 않고 보라색을 타나내는 것을 그람양성균이라 부르며, 포도알균-폐렴알균-사슬알균 등이 대표적인 예이다. 탈색되면 그람음성균이라 부르며, 대장균-녹농균 등이 있다. 최후에 사프라닌, 푹신 등 적색계의 색소액으로 후염색을 한다. 미생물의 감별에 중요한 염색법이며 염색성의 차이는 영양요구성, 화학요법제(항생물질 등)에 대한 감수성의 차이와도 관련되는 점이 많으므로 사용약제의 선택시에도 중요한 역할을 한다. 그람양성균과 음성균의 차이는 세균벽의 구조에 있는데, 양성균은 세포막의 바깥쪽 펩티드글리칸이라는 당단백질층의 두께가 20~80nm나 되고, 그램음성균의 펩티드글리칸 층은 2~3nm로 얇고, 다시 바깥쪽에 외막이라 부르는 층이 있다. |
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| AFB Stain | Acid Fast Bacilli Stain |
|---|---|
| HE stain | Hematoxylin-Eosin stain |
| WS | Waardenburg syndrome; ward secretary; Warkany syndrome; Warthin-Starry [stain]; water soluble; water... |
| cen | centromere; central |
| CENP | centromere protein |
| PWS | Port Wine Stain |
|---|---|
| ASGB | Adjustable Silicone Gastric Banding |
| b | C-banding |
| LASGB | Laparoscopic Adjustable Silicone Gastric Banding |
| PAB | Pulmonary Artery Banding |
| centromere banding stain | <technique> A selective chromosome banding stain used in human cytogenetics, employing Giemsa stain after most of the DNA is denatured or extracted by treatment with alkali, acid, salt, or heat; only heterochromatic regions close to the centromeres and rich in satellite DNA stain, with the exception of the Y chromosome whose long arm usually stains throughout. Synonym: centromere banding stain. (05 Mar 2000) |
|---|
| centromere | <cell biology> The region in eukaryote chromosomes where daughter chromatids are joined together. The kinetochore, to which the spindle chromosomes are attached, lies adjacent to the centromere. The centromeric DNA codes for the kinetochore. (18 Nov 1997) |
|---|---|
| G-banding stain | <technique> A unique chromosome staining technique, used in human cytogenetics to identify individual chromosomes, which produces characteristic bands. It utilises acetic acid fixation, air drying, denaturing chromosomes mildly with proteolytic enzymes, salts, heat, detergents, or urea, and finally Giemsa stain; chromosome bands appear similar to those fluorochromed by Q-banding stain. Synonym: Giemsa chromosome banding stain. (05 Mar 2000) |
| R-banding stain | <technique> A reverse Giemsa chromosome banding method that produces bands complementary to G-bands; induced by treatment with high temperature, low pH, or acridine orange staining; often used together with G-banding on human karyotype to determine whether there are deletions. (05 Mar 2000) |
| Giemsa chromosome banding stain | <technique> A unique chromosome staining technique, used in human cytogenetics to identify individual chromosomes, which produces characteristic bands. It utilises acetic acid fixation, air drying, denaturing chromosomes mildly with proteolytic enzymes, salts, heat, detergents, or urea, and finally Giemsa stain; chromosome bands appear similar to those fluorochromed by Q-banding stain. Synonym: Giemsa chromosome banding stain. (05 Mar 2000) |
| C-banding stain | <technique> A selective chromosome banding stain used in human cytogenetics, employing Giemsa stain after most of the DNA is denatured or extracted by treatment with alkali, acid, salt, or heat; only heterochromatic regions close to the centromeres and rich in satellite DNA stain, with the exception of the Y chromosome whose long arm usually stains throughout. Synonym: centromere banding stain. (05 Mar 2000) |
| quinacrine chromosome banding stain | q-banding stain |
| telomeric R-banding stain | <technique> A modified R-banding stain in which the telomeres become strongly stained and faint R-banding still occurs over the rest of the chromosomes; uses air-dried slides, aging for several days, and staining in hot phosphate-buffered Giemsa stain. (05 Mar 2000) |
| trypsin G-banding stain | <technique> A unique chromosome staining technique, used in human cytogenetics to identify individual chromosomes, which produces characteristic bands. It utilises acetic acid fixation, air drying, denaturing chromosomes mildly with proteolytic enzymes, salts, heat, detergents, or urea, and finally Giemsa stain; chromosome bands appear similar to those fluorochromed by Q-banding stain. Synonym: Giemsa chromosome banding stain. (05 Mar 2000) |
| banding | <dentistry> The process of cementing orthodontic bands to your teeth. (08 Jan 1998) |
| banding of chromosomes | Treatment of chromosomes to reveal characteristic patterns of horizontal bands. Thanks to these banding patterns that resemble bar codes, each human chromosome is distinctive and can be identified without ambiguity. (12 Dec 1998) |
| banding pattern | <genetics> Chromosomes stained with certain dyes, commonly quinacrine (Q banding) or Giemsa (G banding), show a pattern of transverse bands of light and heavy staining that is characteristic for the individual chromosome. The basis of the differential staining, which is the same in most tissues, is not understood: each band represents 5-10% of the length, about 10exp7 base pairs, although this is not true for polytene chromosomes in Drosophila that show more than 4000 bands. (18 Nov 1997) |
| BrDu-banding | The labeling of chromosomes in proliferating tissue by adding an excess of bromodeoxyuridine, which replaces the uridine incorporated in RNA and fluoresces in ultraviolet light; the bands result from sister chromatid exchanges. (05 Mar 2000) |
| G-banding | <genetics> Chromosomes stained with certain dyes, commonly quinacrine (Q banding) or Giemsa (G banding), show a pattern of transverse bands of light and heavy staining that is characteristic for the individual chromosome. The basis of the differential staining, which is the same in most tissues, is not understood: each band represents 5-10% of the length, about 10exp7 base pairs, although this is not true for polytene chromosomes in Drosophila that show more than 4000 bands. (18 Nov 1997) |
| R-banding | See: R-banding stain. (05 Mar 2000) |
| C banding | <molecular biology> Method of defining chromosome structure by staining with Giemsa and looking at the banding pattern in the heterochromatin of the centromeric regions. Giemsa banding (G banding) of the whole chromosome gives higher resolution. Q banding is done with quinacrine. (18 Nov 1997) |
제품명 |
판매사 |
보험코드 | 성분/함량 | 구분/보험급여 |
|---|
제품명 |
판매사 |
보험코드 | 성분/함량 | 구분/보험급여 |
|---|