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| AGE | acrylamide gel; acute gastroenteritis; advanced glycation end product; agarose gel electrophoresis; ... |
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| AGD | agar gel diffusion; agarose diffusion; alpha-ketoglutarate dehydrogenase |
| AE | above-elbow [amputation]; acrodermatitis enteropathica; activation energy; adult erythrocyte; advers... |
| PAGE | Poly-Acrylamide Gel Electrophoresis |
| SDS-PAGE | Sodium DodecylSulfate-PolyacrylAmide Gel Electrophoresis |
| AGE | Agarose gel electrophoresis |
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| BN-PAGE | Blue Native polyacrylamide gel electrophoresis |
| CGE | Capillary Gel Electrophoresis |
| CSGE | Conformation sensitive gel electrophoresis |
| CDGE | Constant denaturant gel electrophoresis |
| agarose gel electrophoresis | <procedure> A type of electrophoresis that uses a matrix of highly purified agar to separate large nucleotides in size. (06 May 1997) |
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| gel electrophoresis | <molecular biology> Electrophoresis using a gel supporting phase. Usually applied to systems where the gel is based on polyacrylamide. See: electrophoresis. (05 May 1997) |
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| polyacrylamide gel electrophoresis | Analytical and separative technique in which molecules, particularly proteins, are separated by their different electrophoretic mobilities in a hydrated gel. The gel suppresses convective mixing of the fluid phase through which the electrophoresis takes place and contributes molecular sieving. Commonly carried out in the presence of the anionic detergent sodium dodecylsulphate (SDS). SDS denatures proteins so that noncovalently associating sub unit polypeptides migrate independently and by binding to the proteins confers a net negative charge roughly proportional to the chain weight. See: SDS PAGE. (21 Jun 1999) |
| pulsed-field gel electrophoresis | Gel electrophoresis in which, after electrophoretic migration has begun, the current is briefly stopped and reapplied in a different orientation; allows for the purification of long DNA molecules. Synonym: pulsed-field gel electrophoresis. (05 Mar 2000) |
| pulse-field gel electrophoresis | Gel electrophoresis in which, after electrophoretic migration has begun, the current is briefly stopped and reapplied in a different orientation; allows for the purification of long DNA molecules. Synonym: pulsed-field gel electrophoresis. (05 Mar 2000) |
| electrophoresis, agar gel | Electrophoresis in which agar or agarose gel is used as the diffusion medium. (12 Dec 1998) |
| electrophoresis, gel, pulsed-field | Electrophoresis in which the direction of the electric field is changed periodically. This technique is similar to other electrophoretic methods normally used to separate double-stranded DNA molecules ranging in size up to tens of thousands of base-pairs. However, by alternating the electric field direction one is able to separate DNA molecules up to several million base-pairs in length. (12 Dec 1998) |
| electrophoresis, gel, two-dimensional | Electrophoresis in which a second perpendicular electrophoretic transport is performed on the separate components resulting from the first electrophoresis. This technique is usually performed on polyacrylamide gels. (12 Dec 1998) |
| electrophoresis, polyacrylamide gel | Electrophoresis in which a polyacrylamide gel is used as the diffusion medium. (12 Dec 1998) |
| electrophoresis, starch gel | Electrophoresis in which a starch gel (a mixture of amylose and amylopectin) is used as the diffusion medium. (12 Dec 1998) |
| two dimensional gel electrophoresis | <technique> A high resolution separation technique in which protein samples are separated by isoelectric focussing in one dimension and then laid on an SDS gel for size determined separation in the second dimension. Can resolve hundreds of components on a single gel. (18 Nov 1997) |
| agarose | <chemical> This linear galactan is created by purifying agar, when it is heated and cooled, it forms a gel that is used as a support for many types of electrophoresis and immunodiffusion (agarbiose). A typical gel is about 1% agarose. Agarose is more porous than acrylamide and is sold in different grades, the lower its sulphate content, the more highly purified it is. (06 May 1997) |
| chromatography, agarose | A method of gel filtration chromatography using agarose, the non-ionic component of agar, for the separation of compounds with molecular weights up to several million. (12 Dec 1998) |
| agar-gel reaction | <immunology> The reaction between an antibody and an antigen during an immunology lab procedure where the two are allowed to diffuse toward each other through an agar-gel medium. Lines of precipitation form in the places on the gel where the two react with each other and shows where the reaction has occurred. (09 Oct 1997) |
| aluminum hydroxide gel | A suspension containing Al2O3, mainly in the form of aluminum hydroxide, used as an antacid; a dried form, with the same use, is obtained by drying the product of interaction in aqueous solution of an aluminum salt with ammonium or sodium carbonate. (05 Mar 2000) |
| aluminum phosphate gel | An aqueous suspension of between 4.0 and 5.0% of aluminum phosphate; used as an antacid. (05 Mar 2000) |
| agarose gel electrophoresis |
Agarose gel electrophoresis is a method used in molecular biology to separate DNA strands by size, and to determine the size of the separated strands by comparison to strands of known length. It operates by a mechanism similar to sifting molecules through a sieve; an electric field is used to drag negatively charged DNA molecules through a gel matrix, and the shorter DNA molecules move faster than the longer ones since they are able to slip through the gel more easily. ...
Ãâó: en.wikipedia.org/wiki/Agarose_gel_electrophoresis
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| agarose gel electrophoresis |
A technique used to separate DNA fragments (and proteins) by their size. An electric current is used to propel the DNA (or proteins) through a porous gel matrix.
Ãâó: www.bscs.org/onco/glossary.htm
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