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"Southern blot test"¿¡ ´ëÇÑ °Ë»ö °á°úÀÔ´Ï´Ù. °Ë»ö °á°ú º¸´Â µµÁß¿¡ Tab ۸¦ ´©¸£½Ã¸é °Ë»ö âÀÌ ¼±Åõ˴ϴÙ.
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¿µ¹® test ÇÑ±Û °Ë»ç
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  ¾î¶² ´Ù¸¥ ¹°ÁúÀ» °ËÃâ, ÃøÁ¤, »ý¼ºÇϱâ À§ÇѠƯÁ¤ÇÑ È­ÇйÝÀÀÀ» ÀÏÀ¸Å°´Âµ¥ »ç¿ëµÇ´Â ¹æ¹ý.
¿µ¹® scratch test ÇÑ±Û ³­Àý¹ý
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  ÇǺθ¦ ³¯Ä«·Î¿î ¹Ù´Ã·Î ±Ü¾î ÇǺÎÀÇ ¹ÝÀÀÀ» º¸´Â °Ë»ç·Î ÇǺΠ°ú¹Î¹ÝÀÀÀ̳ª ¾Ë·¹¸£±â¸¦ ¾Ë¾Æº¸±â À§ÇÑ °Ë»çÀÌ´Ù. ¹Ù´Ã³¡¿¡ Ç׿øÀ» ¹¯Èù µÚ, ÇǺιØÀ» ±Ü¾î ¹ÝÀÀÀ» ¾Ë¾Æº»´Ù. À̶§ Ç׿øÀÌ ¾Æ´Ñ ´ëÁ¶¹°Áú(¿¹¸¦ µé¾î º¸ÅëÀÇ ¹°)À» ¹¯Èù ¹Ù´ÃÀ» °°ÀÌ ¹ÝÀÀÇÏ¿© ÇǺ馱âÁõ(dermographism) ´ÜÁö ¹Ù´ÃÀÇ ±ÜÈû¸¸À¸·Î ¾Ë·¹¸£±â °°Àº ¹ÝÀÀÀ» ÀÏÀ¸Å°´Â Çö»ó°ú °¨º°ÇؾߠÇÑ´Ù.
  
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¿µ¹® stool guaiac test ÇÑ±Û ´ëº¯ ±¸¾ÆÀÌ¾Ç °Ë»ç
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  ´ëº¯³»¿¡ ÀÖÀ» ¼ö Àִ ÀáÇ÷(´«¿¡ º¸ÀÌÁö ¾Ê´Â ÃâÇ÷)À¯¹«¸¦ °Ë»çÇϴ ¹æ¹ýÀ¸·Î, Ç÷±¸³»ÀÇ heme peroxidase¿¡ ÀÇÇØ guaiacÀÌ »êÈ­µÇ´Â ¹ÝÀÀÀ» ÀÌ¿ëÇÏ¿© ÃøÁ¤ÇÑ´Ù. ¹æ¹ýÀº 3Àϰ£¿¡ °ÉÃÄ ÇÑ º¯¿¡¼­ 2±ºµ¥¾¿ Ã¤ÃëÇÏ¿© °Ë»çÇÑ´Ù. À§¾ç¼º ¹ÝÀÀ(°ÅÁþÀ¸·Î Ç÷¾×ÀÌ ÀÖ´Ù°í ³ªÅ¸³ª´Â ¹ÝÀÀ)Àº ½Ä¹° °ú»êÈ­È¿¼Ò¸¦ ÇÔÀ¯Çϰí Àִ ȫ´ç¹«ÀÇ ¼·Ã볪 Ç÷±¸ ¼ººÐÀ» ÇÔÀ¯Çϰí Àִ °í±â ¼·Ã렵¼­ ³ªÅ¸³¯ ¼ö ÀÖÀ¸¸ç, À§À½¼º ¹ÝÀÀ(½ÇÁ¦·Î Ç÷¾×Àº ÀÖÁö¸¸, Ç÷¾×ÀÌ ¾ø´Ù°í ³ªÅ¸³ª´Â ¹ÝÀÀ)Àº È¯¿ø·ÂÀ» °¡Áö°í Àִ ºñŸ¹Î CÀÇ º¹¿ë½Ã ³ªÅ¸³¯ ¼ö ÀÖ´Ù. Æ¯È÷ À§¾ç¼º ¹ÝÀÀÀÌ ¸Å¿ì ÈçÇÏ´Ù.
¿µ¹® Rorschach Test ÇÑ±Û ·Î¸£»þÇÏ °Ë»ç
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  »ç°íÀå¾Ö¿Í Á¤¼­Àå¾Ö¿¡ ¹Î°¨ÇÑ Åõ»ç°Ë»ç(projective test). °ËÀº»ö°ú ¸î°¡Áö »öÀ¸·Î ÀÌ·ç¾îÁø À×Å©¾ó·è°°Àº µµÇüÀÌ ±×·ÁÁø 10°³ÀÇ Ä«µå¸¦ ÀÌ¿ëÇÑ´Ù. ÇǰËÀÚ¿¡°Ô Ä«µå¸¦ º¸ÀÌ°í º» °Í¿¡ ´ëÇØ ¸»Çϵµ·Ï ÇÑ´Ù. ´ÙÀ½¿¡´Â ¾ó·èÀÇ ¾î´À À§Ä¡°¡ ÇǰËÀÚ°¡ ¸»ÇÑ Áö°¢´ë»óÀ» ¾Ï½ÃÇÏ´ÂÁö Ã£µµ·Ï ÇÑ´Ù. ÇǰËÀÚÀÇ ´äº¯À» ºÐ¼®Çϸ頱×ÀÇ »ç°í¿Í Á¤¼­»óÅ¿¡ ´ëÇÑ Á¤º¸¸¦ ¾òÀ» ¼ö ÀÖ´Ù. 
¿µ¹® Mantoux test ÇÑ±Û ¸ÁÅä¿ì°Ë»ç
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  ¼Ò¾Æ¿¡ ÁַΠ½Ç½ÃÇϴ °Ë»ç ¹æ¹ýÀ¸·Î, BCGÁ¢Á¾ÈÄ¿¡³ª È¤Àº °áÇÙ±Õ¿¡ ÇѹøÀ̶󵵠³ëÃâµÈ °æ¿ì¿¡ ¾ç¼º¹ÝÀÀÀ¸·Î ³ª¿Â´Ù. ¿ì¸®³ª¶ó¿¡¼­´Â »ýÈÄ 1°³¿ù¿¡ BCG¿¹¹æÁ¢Á¾À» ½ÃÇàÇϹǷΠ´ëºÎºÐ ¾ç¼ºÀ¸·Î ³ª¿À°Ô µÇ³ª, ÀÌ ¿¹¹æÁ¢Á¾À» ¹ÞÁö ¾ÊÀº »óÅ¿¡¼­ ¾ç¼ºÀ¸·Î ³ª¿À¸é, °áÇÙ±ÕÀǠħÅõ¸¦ ÀǽÉÇÒ ¼ö ÀÖ´Ù.
´ëÇÑÀÇÇù ÀÇÇпë¾î »çÀü °Ë»ö À¯»ç °Ë»ö °á°ú : 15 ÆäÀÌÁö: 1
  • ¿µ¹®
    ÇѱÛ
  • Southern blot analysis
    ¼­´øºí·ÔºÐ¼®
  • dot blot test
    Á¡Àû°Ë»ç, ¾ó·èÁ¡°Ë»ç
  • Western blot test
    ¿þ½ºÅϺí·Ô°Ë»ç
  • blot
    1. Á¡, ¾ó·è 2. ºí·Ô¹ý
  • blot hemorrhage
    ¹ÝÁ¡ÃâÇ÷
  • enzyme-linked immunoelectrotransfer blot
    È¿¼Ò°áÇո鿪ÀÌÀû¹ý, È¿¼Ò¿¬°ü¸é¿ªÀü±âÀü´Þ¹ý
  • Northern blot analysis
    ³ë´øºí·ÔºÐ¼®
  • Western blot analysis
    ¿þ½ºÅϺí·ÔºÐ¼®
  • acceptance test
    Àμö°Ë»ç
  • antibody identification test
    Ç×üȮÀΰ˻ç
  • antibody screening test
    Ç×ü¼±º°°Ë»ç
  • anticholinesterase test
    Ç×Äݸ°¿¡½ºÅ×¶ó¾ÆÁ¦°Ë»ç
  • antiglobulin test
    Çױ۷κҸ°°Ë»ç
  • antimicrobial susceptibility test
    Ç×±ÕÁ¦°¨¼ö¼º°Ë»ç
  • antinuclear antibody test
    Ç×ÇÙÇ×ü°Ë»ç
´ëÇÑÀÇÇù Çʼö ÀÇÇпë¾îÁý »çÀü °Ë»ö À¯»ç °Ë»ö °á°ú : 15 ÆäÀÌÁö: 1
  • ¿µ¹®
    ÇѱÛ
  • nitroblue tetrazolium test
    ´ÏÆ®·Îºí·çÅׯ®¶óÁ¹·ý°Ë»ç
  • test strip
    °Ë»ç¶ì
  • test
    °Ë»ç, ½ÃÇè
  • absorption test
    Èí¼ö½ÃÇè, Èí¼ö°Ë»ç
  • agglutination test
    ÀÀÁý°Ë»ç
  • antibody screening test
    Ç×ü¼±º°°Ë»ç
  • anticholinesterase test
    Ç×Äݸ°¿¡½ºÅ×¶óÁ¦°Ë»ç
  • antiglobulin test
    Çױ۷κҸ°°Ë»ç
  • antimicrobial susceptibility test
    Ç×±ÕÁ¦°¨¼ö¼º°Ë»ç
  • blood test
    Ç÷¾×°Ë»ç
  • blood coagulation test
    Ç÷¾×ÀÀ°í°Ë»ç
  • blood sugar test
    Ç÷´ç°Ë»ç
  • bronchial provocation test
    ±â°üÁöÀ¯¹ß°Ë»ç
  • contraction stress test
    ¼öÃàÀڱذ˻ç
  • Coombs' test
    (¢¡antiglobulin test) ÄñÁî°Ë»ç
¿¾ ´ëÇÑÀÇÇù ÀÇÇпë¾î »çÀü °Ë»ö À¯»ç °Ë»ö °á°ú : 15 ÆäÀÌÁö: 1
  • ¿µ¹®
    ÇѱÛ
  • Southern blot analysis
    ¼­´øºí·ÔºÐ¼®
  • dot blot test
    Á¡Àû°Ë»ç, ¾ó·èÁ¡°Ë»ç
  • Southern hybridization
    ¼­´øºÎÇÕ¹ý
  • blot
    Á¡, ¾ó·è
  • blot hemorrhage
    ¹ÝÁ¡ÃâÇ÷
  • enzyme-linked immunoelectrotransfer blot
    È¿¼Ò¸é¿ªÀÌÀû¹ý, È¿¼Ò¿¬°ü¸é¿ªÀü±âÀü´ÞÁ¡
  • dot blot hybridization
    ¹ÝÁ¡Å¹º»ºÎÇÕ¹ý
  • abrasion test
    ¸¶¸ê½ÃÇè
  • absorption test
    Èí¼ö½ÃÇè, Èí¼ö°Ë»ç
  • acceptance test
    Àμö°Ë»ç
  • achievement test
    ¼ºÃëµµ°Ë»ç
  • acid challenge test
    »êÅõ¿©°Ë»ç
  • acid elution slide test
    »ê¿ëÃâ½½¶óÀ̵å°Ë»ç
  • adsorption test
    ÈíÂø½ÃÇè, ºÎÂø°Ë»ç
  • afterimage test
    ÀÜ»ó°Ë»ç
¿¾ ´ëÇÑÀÇÇù 2 ÀÇÇпë¾î »çÀü °Ë»ö ¸ÂÃã °Ë»ö °á°ú : 1 ÆäÀÌÁö: 1
  • ¿µ¹®
    ÇѱÛ
  • Southern blot test
    ¼­´øºí·Ô½ÃÇè
¿¾ ´ëÇÑÀÇÇù 2 ÀÇÇпë¾î »çÀü °Ë»ö À¯»ç °Ë»ö °á°ú : 15 ÆäÀÌÁö: 1
  • ¿µ¹®
    ÇѱÛ
  • Western blot test
    ¿þ½ºÅϺí·Ô½ÃÇè
  • immunoelectrophoresis Western blot test
    ¸é¿ªÀü±â¿µµ¿ ¿þ½ºÅϺí·ÎÆ®½ÃÇè
  • Southern blotting
    ¼­´ø ºí·µÆÃ
  • hybridization, Southern
    ¼­´øºÎÇÕ¹ý(ݬùêÛö)
  • Northern blot
    ³ë´ø ¹ÝÁ¡Âï±âºÐ¼®¹ý, ³ë´øÅ¹º»ºÎÇÕ¹ý(~ݬùêÛö)
  • Western blot
    ¿þ½ºÅϺҷµÆ®
  • hybridization, dot blot
    ¹ÝÁ¡Å¹º»ºÎÇÕ¹ý(~ݬùêÛö), ¹ÝÁ¡Ç¥Áö¹ý
  • G-test=£¾gonadotropin stimulation test
    °í³ªµµÆ®·ÎÇÉ ÀÚ±ØÅ×½ºÆ®. µµÆ®·ÎÇÉÁö±Ø°Ë»ç
  • Ham test=acidified serum test
    ÇÜ °Ë»ç, »ê¼ºÈ­Ç÷û¿ëÇ÷°Ë»ç
  • RPR test => rapid plasma reagin test
    ½Å¼ÓÇ÷À帮¾ÆÁø<°¨ÀÛÇ×ü>½ÃÇè
  • VDRL test= venereal disease research laboratory test
    ¼ºº´¿¬±¸¼Ò½ÄÅ×½ºÆ®.
  • VDRL test= venereal disease research laboratory test
    ¼ºº´¿¬±¸¼Ò½ÄÅ×½ºÆ®.
  • acidified serum test=Ham test
    »ê¼ºÈ­Ç÷û°Ë»ç
  • acidified-serum lysis test=Ham test
    »ê¼ºÈ­Ç÷û¿ëÇ÷°Ë»ç
  • antiglobulin test = Coombs test
    Çױ۷κҸ°°Ë»ç
¿¾ ´ëÇÑÀÇÇù 3 ÀÇÇпë¾î »çÀü °Ë»ö À¯»ç °Ë»ö °á°ú : 15 ÆäÀÌÁö: 1
  • ¿µ¹®
    ÇѱÛ
  • southern blot anlysis
    ¼­´øºí¶óÆ®ºÐ¼®(¡­ÝÂà°)
  • dot blot test
    Á¡Àû(ïÇîÙ)½ÃÇè, Á¡Àû¹ý
  • immunoelectrophoresis Western blot test
    ¸é¿ªÀü±â¿µµ¿ ¿þ½ºÅϺí·ÎÆ®½ÃÇè
  • hybridization, Southern
    ¼­´øºÎÇÕ¹ý(ݬùêÛö)
  • southern blotting
    ½á´øºí·Ô
  • southern blotting
    ½á´ø ºí·ÔÆÃ
  • blot hemorrhage
    ¹ÝÁ¡ÃâÇ÷
  • enzyme-linked immunoelectrotransfer blot
    È¿¼Ò¿¬°ü ¸é¿ªÀü±âÀü´ÞÁ¡
  • hybridization, dot blot
    ¹ÝÁ¡Å¹º»ºÎÇÕ¹ý(~ݬùêÛö), ¹ÝÁ¡Ç¥Áö¹ý
  • nethern blot
  • acidified serum test=Ham test
    »ê¼ºÈ­Ç÷û°Ë»ç
  • acidified-serum lysis test=Ham test
    »ê¼ºÈ­Ç÷û¿ëÇ÷°Ë»ç
  • antiglobulin test = Coombs test
    Çױ۷κҸ°°Ë»ç
  • biologic test ; biological test
    »ý¹°ÇÐÀû ½ÃÇè(¡­ãËúÐ).
  • bromosulfalein test =BSF test
    ºê·Ò¼úÆÛ·¹ÀνÃÇè.
´ëÇѱâ»ýÃæÇÐȸ ÀÇÇпë¾î »çÀü °Ë»ö À¯»ç °Ë»ö °á°ú : 10 ÆäÀÌÁö: 1
  • ¿µ¹®
    ÇѱÛ
  • enzyme-linked immunoelectrotransfer blot (EITB)
    È¿¼Ò¸é¿ªÀÌÀû¹ý
  • Bachman intradermal test
    ¼±¸ðÃæÇdz»¹ÝÀÀ°Ë»ç
  • bentonite flocculation test
    º¥Å䳪ÀÌÆ®ÀÀÁý°Ë»ç
  • Casoni test
    Æ÷ÃæÇdz»¹ÝÀÀ°Ë»ç
  • circumoval precipitin test (COPT)
    Ãæ¶õÁÖÀ§Ä§°­°Ë»ç
  • intradermal test
    Çdz»¹ÝÀÀ°Ë»ç
  • Mazzotti test
    ¸¶Á¶Æ¼°Ë»ç
  • Montenegro test
    ¸óÅ״ϱ׷ΰ˻ç
  • Sabin-Feldman dye test
    (Åå¼ÒÆ÷ÀÚÃæ) »ö¼Ò¸é¿ª°Ë»ç
  • screening test
    ¼±º°°Ë»ç
´ëÇÑ»ýÈ­ÇкÐÀÚ»ý¹°ÇÐȸ ¿ë¾î »çÀü °Ë»ö À¯»ç °Ë»ö °á°ú : 15 ÆäÀÌÁö: 1
  • ¿µ¹®
    ÇѱÛ
  • Southern blotting
    ¼­´ø ºí·ÔÆÃ
  • blot hybridization
    ºí·Ô Æ¢±âÇü¼º(û¡à÷)
  • DNA dot blot
    DNA Á¡(ïÃ)ºí·Ô
  • dot blot assay
    Á¡(ïÃ)ºí·Ô ¾Æ¼¼ÀÌ
  • gel blot hybridization
    Á© ºí·Ô Æ¢±âÇü¼º(û¡à÷)
  • RNA dot blot
    RNA Á¡(ïÇ)ºí·Ô
  • acrolein test
    ¾ÆÅ©·Ñ·¹ÀνÃÇè (ãËúÐ)
  • acute test
    ´Ü·® µ¶¼º½ÃÇè (Ó¤ÕáÔ¸àõãËúÐ)
  • adrenaline tolerance test
    ¾Æµå·¹³¯¸° ºÎÇϽÃÇè(ݶùÃãËúÐ)
  • albustix test
    ¾ËºÎ½ºÆ½½º½ÃÇè(ãËúÐ)
  • Ames test
    ¿¡ÀÓÁî °Ë»ç¹ý
  • antiglobulin consumption test
    Ç×(ù÷)±Û·ÎºÒ¸° ¼Ò¸ð½ÃÇè(á¼ÙÄãËúÐ)
  • antiglobulin test
    Ç×(ù÷)±Û·ÎºÒ¸° ½ÃÇè(ù÷ô÷ãËúÐ)
  • Aschheim-Zondek test
    ¾Æ»þÀÓ-Á¸µ¦ ½ÃÇè(ãËúÐ)
  • Ascoli test
    ¾Æ½ºÄݸ® ½ÃÇè(ãËúÐ)
KI ÀÇÇпë¾î »çÀü °Ë»ö À¯»ç °Ë»ö °á°ú : 15 ÆäÀÌÁö: 1
  • ¿µ¹®
    ÇѱÛ
  • pelvis perfusion test(Whitaker test)
    ½Å¿ì°ü·ù°Ë»ç
  • arm to lung time test
    ÆÈÆó¼øÈ¯½Ã°£½ÃÇè
  • chi-square test
    Ä«ÀÌÁ¦°ö°ËÁ¤
  • complement fixation test
    º¸Ã¼°áÇÕ½ÃÇè
  • contraction test
    ¼öÃà°Ë»ç
  • double blind test
    ÀÌÁ߸Ͱ˹ý
  • dye dilution test
    »ö¼ÒÈñ¼®½ÃÇè
  • guaiac test
    °úÀ̾׽ÃÇè
  • hemagglutination inhibition test
    Ç÷±¸ÀÀÁý¾ïÁ¦½ÃÇè
  • laboratory test
    °Ë»ç
  • pivot shift test
    ÃßÃà º¯À§½ÃÇè
  • provocation test
    À¯¹ß½ÃÇè
  • provocative test
    À¯¹ß½ÃÇè
  • pulmonary function test
    Æó±â´É°Ë»ç
  • qualitative test
    Á¤¼º½ÃÇè
KMLE ÀÇÇоà¾î »çÀü À¯»ç °Ë»ö °á°ú : 5 ÆäÀÌÁö: 1
SCPNT Southern California Postrotary Nystagmus Test
SCSIT Southern California Sensory Integration Test
PAT Pain Apperception Test; paroxysmal atrial tachycardia; patient; phenylaminotetrazole; physical abili...
IBT ink blot test
STSA Southern Thoracic Surgical Association
KMLE ÀÚµ¿ÃßÃâ ÀÇÇоà¾î »çÀü À¯»ç °Ë»ö °á°ú : 5 ÆäÀÌÁö: 1
SB Southern Blot
SBH Southern blot hybridisation
ENSO El Nino Southern Oscillation
SBMV Southern bean mosaic virus
SB Southern blotting
°æºÏ´ë Ä¡°ú´ëÇÐ ±¸°­³»°ú ±³½Ç »çÀü À¯»ç °Ë»ö °á°ú : 15 ÆäÀÌÁö: 1
  • ¿µ¹®
    ÇѱÛ
    ¼³¸í
  • dot blot test
    Á¡Àû ½ÃÇè, Á¡Àû¹ý
    southern blotting³ª northern blotting°ú´Â ´Þ¸® Á¦ÇÑ È¿¼Ò¸¦ »ç¿ëÇϰųª Å©±âº°·Î ÀÚ¸£Áö ¾ÊÀ¸¸ç ¸Å¿ì ºü¸£´Ù´Â ÀåÁ¡ÀÌ ÀÖ´Ù. Á¶»çÇϰíÀÚ Çϴ ȯÀÚÀÇ DNA ½Ã·á ¶Ç´Â °Ë»çÇÒ RNA¸¦ ¸· À§¿¡ Á¡ ¸ð¾çÀ¸·Î ¿Ã·Á³õ¾Æ
  • abrasion test
    ¸¶¸ê ½ÃÇè
    ¸¶¸ð¿¡ °ßµð´Â Á¤µµ¸¦ ½ÃÇèÇÏ´Â °Ë»ç.
  • acetest tablet test
    ¾Æ¼¼Å×½ºÆ® Á¤ ½ÃÇè
    ¹éÁö À§¿¡ ¿Ã·Á³õÀº ¾Æ¼¼Å×½ºÆ® Á¤¿¡ ¿ä ÇÑ ¹æ¿ïÀ» ¶³¾î¶ß¸± ¶§, ¾î´À Á¤µµÀÇ ¾Æ¼¼ÅæÀÌ Á¸ÀçÇÏ¸é ±× ¾ç¿¡ µû¶ó¼­ ¾Æ¼¼Å×½ºÆ® Á¤ÀÌ ´ãÀÚ»ö
  • acetoacetic acid test
    ¾Æ¼¼Åä ÃÊ»ê ½ÃÇè
  • achievement test
    ¼öµæ´É·Â °Ë»ç, ¼öµæ´É·Âµµ °Ë»ç
  • acid elution test
    »ê ¿ë¸® ½ÃÇè
    ÅÂ¾Æ Çì¸ð±Û·ÎºóÀÇ °ËÃâ ½ÃÇèÀ¸·Î, ½½¶óÀÌµå ±Û¶ó½º À§¿¡ °ø±â °ÇÁ¶µÈ Ç÷¾× µµÆ÷ Ç¥º»À» 80% ¸ÞŸ³î·Î °íÁ¤ÇÏ¿©,
  • acoustic reflex test
    û°¢¹Ý»ç ½ÃÇè
    û°¢¹Ý»ç ¿ªÄ¡ÀÇ ÃøÁ¤¹ýÀ¸·Î¼­ Àüµµ¼º°ú °¨°¢½Å°æ¼º ³­Ã»À» °¨º° Áø´ÜÇϰųª û½Å°æÁ¾À» Áø´ÜÇÏ´Â µ¥ ÀÌ¿ëµÈ´Ù.
  • ACTH stimulation test
    ACTH ÀÚ±Ø °Ë»ç
    Çϼöü ºÎ½ÅÇÇÁú°è ¿¹ºñ·Â°Ë»çÀÇ Çϳª. ºÎ½ÅÇÇÁúÀÚ±Ø È£¸£¸ó
  • Addis test
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  • adsorption test
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blot, southern A common test for checking for a match between DNA molecules. DNA fragments are separated by agarose gel electrophoresis, transferred (blotted) onto membrane filters, and hybridised with complementary radiolabelled probes. The aim is to detect specific base sequenceswith the probes. Lest all of this sound esoteric, note that in the television series The X Files a Southern blot was done (in this case, to learn if some alien virus genome had been integrated into a person's genome). The Southern blot is named after its inventor, the British biologist M.E. Southern. There is also a Northern blot and a Western blot.
(12 Dec 1998)
southern blot A technique used for searching for a specific DNA fragment. The process is as follows:
1. separate DNA fragments by gel electrophoresis
2. Change pH of gel to basic, thus allowing disruption of H-bonds
3. blot gel with nitrocellulose paper
4. Heat paper so as to fix DNA fragments
5. probe with labelled messenger RNA or cDNA
6. wash
Complementary mRNA/cDNA fragments will have hybridised.
(09 Oct 1997)
Southern blot analysis A procedure to separate and identify DNA sequences; DNA fragments are separated by electrophoresis on an agarose gel, transferred (blotted) onto a nitrocellulose or nylon membrane, and hybridised with complementary (labelled) nucleic acid probes.
(05 Mar 2000)
africa, southern The geographical area of africa comprising angola, botswana, lesotho, malawi, mozambique, namibia, south africa, swaziland, zambia, and zimbabwe. It includes what was formerly called south-west africa or german southwest africa but it was terminated in 1966 by a united nations resolution.
(12 Dec 1998)
blotting, southern A method (first developed by e.m. Southern) for detection of DNA that has been electrophoretically separated and immobilised by blotting on nitrocellulose or other type of paper or nylon membrane.
(12 Dec 1998)
Southern M.E., 20th century British biologist.
See: Southern blot analysis.
(05 Mar 2000)
Southern blotting <molecular biology, procedure> General term for the transfer of protein, RNA or DNA molecules from a relatively thick acrylamide or agarose gel or to a paper like membrane (usually nylon or nitrocellulose) by capilliarity or an electric field, preserving the spatial arrangment. Once on the membrane, the molecules are immobilised, typically by baking or by ultra violet irradiation and can then be detected at high sensitivity by hybridisation (in the case of DNA & RNA) or antibody labelling (in the case of protein). RNA blots are called Northern blots, DNA blots, Southern, protein blots, Western.
(18 Nov 1997)
blot 1. To spot, stain, or bespatter, as with ink. "The brief was writ and blotted all with gore." (Gascoigne)
2. To impair; to damage; to mar; to soil. "It blots thy beauty, as frosts do bite the meads." (Shak)
3. To stain with infamy; to disgrace. "Blot not thy innocence with guiltless blood." (Rowe)
4. To obliterate, as writing with ink; to cancel; to efface; generally with out; as, to blot out a word or a sentence. Often figuratively; as, to blot out offenses. "One act like this blots out a thousand crimes." (Dryden)
5. To obscure; to eclipse; to shadow. "He sung how earth blots the moon's gilded wane." (Cowley)
6. To dry, as writing, with blotting paper.
Synonym: To obliterate, expunge, erase, efface, cancel, tarnish, disgrace, blur, sully, smear, smutch.
Origin: Cf. Dan. Plette. See Blot.
Source: Websters Dictionary
(01 Mar 1998)
blot, northern A technique in molecular biology, used mainly to separate and identify pieces of RNA. Called a Northern blot only because it is similar to a Southern blot (which is named after its inventor, the British biologist M.E. Southern).
(12 Dec 1998)
blot, western A technique in molecular biology, used to separate and identify proteins. Called a Western blot merely because it has some similarity to a Southern blot (which is named after its inventor, the British biologist M.E. Southern).
(12 Dec 1998)
western blot <technique> A technique similar to Southern blotting, though it is used for proteins.
(09 Oct 1997)
Western blot analysis A procedure in which proteins separated by electrophoresis in polyacrylamide gels are transferred (blotted) onto nitrocellulose or nylon membranes and identified by specific complexing with antibodies that are either pre-or post-tagged with a labelled secondary protein.
See: immunoblot.
Synonym: Western blot, Western blotting.
Origin: coined to distinguish it from eponymic Southern blot a.
(05 Mar 2000)
Northern blot <molecular biology, procedure> An electroblotting method in which RNA is transferred to a filter and detected by hybridisation to (32)P labelled RNA or DNA.
(18 Nov 1997)
Northern blot analysis A procedure similar to the Southern blot analysis, used mostly to separate and identify RNA fragments; typically via transferring RNA fragments from an agarose gel to a nitrocellulose filter followed by detection with a suitable probe.
Origin: coined to distinguish it from eponymic Southern blot a.
(05 Mar 2000)
dot blot <molecular biology, procedure> Method for detecting a specific protein or message. A spot of solution is dotted onto nitrocellulose paper, a specific antibody or probe is allowed to bind and the presence of bound antibody/probe then shown by using a peroxidase coupled second antibody, as in Western blotting or by other visualisation methods.
(18 Nov 1997)
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Southern Blot test An analytical method traditionally used in DNA analysis. After a sample of DNA fragments is separated by agarose gel electrophoresis, the fragments are transferred to a solid cellulose support by blotting. The gel is placed be
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