| ART | absolute retention time; Accredited Record Technician; acoustic reflex test; algebraic reconstructio... |
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| CT | calcitonin; calf testis; cardiac tamponade; cardiothoracic [ratio]; carotid tracing; carpal tunnel; ... |
| SPT | secretin-pancreazymin [test]; single patch technique; sleep period time; spectrin; station pull-thro... |
| SCPNT | Southern California Postrotary Nystagmus Test |
| SCSIT | Southern California Sensory Integration Test |
| SB | Southern Blot |
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| SBH | Southern blot hybridisation |
| ENSO | El Nino Southern Oscillation |
| SBMV | Southern bean mosaic virus |
| SB | Southern blotting |
| blot, southern | A common test for checking for a match between DNA molecules. DNA fragments are separated by agarose gel electrophoresis, transferred (blotted) onto membrane filters, and hybridised with complementary radiolabelled probes. The aim is to detect specific base sequenceswith the probes. Lest all of this sound esoteric, note that in the television series The X Files a Southern blot was done (in this case, to learn if some alien virus genome had been integrated into a person's genome). The Southern blot is named after its inventor, the British biologist M.E. Southern. There is also a Northern blot and a Western blot. (12 Dec 1998) |
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| southern blot | A technique used for searching for a specific DNA fragment. The process is as follows: 1. separate DNA fragments by gel electrophoresis 2. Change pH of gel to basic, thus allowing disruption of H-bonds 3. blot gel with nitrocellulose paper 4. Heat paper so as to fix DNA fragments 5. probe with labelled messenger RNA or cDNA 6. wash Complementary mRNA/cDNA fragments will have hybridised. (09 Oct 1997) |
| Southern blot analysis | A procedure to separate and identify DNA sequences; DNA fragments are separated by electrophoresis on an agarose gel, transferred (blotted) onto a nitrocellulose or nylon membrane, and hybridised with complementary (labelled) nucleic acid probes. (05 Mar 2000) |
| africa, southern | The geographical area of africa comprising angola, botswana, lesotho, malawi, mozambique, namibia, south africa, swaziland, zambia, and zimbabwe. It includes what was formerly called south-west africa or german southwest africa but it was terminated in 1966 by a united nations resolution. (12 Dec 1998) |
| blotting, southern | A method (first developed by e.m. Southern) for detection of DNA that has been electrophoretically separated and immobilised by blotting on nitrocellulose or other type of paper or nylon membrane. (12 Dec 1998) |
| Southern | M.E., 20th century British biologist. See: Southern blot analysis. (05 Mar 2000) |
| Southern blotting | <molecular biology, procedure> General term for the transfer of protein, RNA or DNA molecules from a relatively thick acrylamide or agarose gel or to a paper like membrane (usually nylon or nitrocellulose) by capilliarity or an electric field, preserving the spatial arrangment. Once on the membrane, the molecules are immobilised, typically by baking or by ultra violet irradiation and can then be detected at high sensitivity by hybridisation (in the case of DNA & RNA) or antibody labelling (in the case of protein). RNA blots are called Northern blots, DNA blots, Southern, protein blots, Western. (18 Nov 1997) |
| blot | 1. To spot, stain, or bespatter, as with ink. "The brief was writ and blotted all with gore." (Gascoigne) 2. To impair; to damage; to mar; to soil. "It blots thy beauty, as frosts do bite the meads." (Shak) 3. To stain with infamy; to disgrace. "Blot not thy innocence with guiltless blood." (Rowe) 4. To obliterate, as writing with ink; to cancel; to efface; generally with out; as, to blot out a word or a sentence. Often figuratively; as, to blot out offenses. "One act like this blots out a thousand crimes." (Dryden) 5. To obscure; to eclipse; to shadow. "He sung how earth blots the moon's gilded wane." (Cowley) 6. To dry, as writing, with blotting paper. Synonym: To obliterate, expunge, erase, efface, cancel, tarnish, disgrace, blur, sully, smear, smutch. Origin: Cf. Dan. Plette. See Blot. Source: Websters Dictionary (01 Mar 1998) |
| blot, northern | A technique in molecular biology, used mainly to separate and identify pieces of RNA. Called a Northern blot only because it is similar to a Southern blot (which is named after its inventor, the British biologist M.E. Southern). (12 Dec 1998) |
| blot, western | A technique in molecular biology, used to separate and identify proteins. Called a Western blot merely because it has some similarity to a Southern blot (which is named after its inventor, the British biologist M.E. Southern). (12 Dec 1998) |
| western blot | <technique> A technique similar to Southern blotting, though it is used for proteins. (09 Oct 1997) |
| Western blot analysis | A procedure in which proteins separated by electrophoresis in polyacrylamide gels are transferred (blotted) onto nitrocellulose or nylon membranes and identified by specific complexing with antibodies that are either pre-or post-tagged with a labelled secondary protein. See: immunoblot. Synonym: Western blot, Western blotting. Origin: coined to distinguish it from eponymic Southern blot a. (05 Mar 2000) |
| Northern blot | <molecular biology, procedure> An electroblotting method in which RNA is transferred to a filter and detected by hybridisation to (32)P labelled RNA or DNA. (18 Nov 1997) |
| Northern blot analysis | A procedure similar to the Southern blot analysis, used mostly to separate and identify RNA fragments; typically via transferring RNA fragments from an agarose gel to a nitrocellulose filter followed by detection with a suitable probe. Origin: coined to distinguish it from eponymic Southern blot a. (05 Mar 2000) |
| dot blot | <molecular biology, procedure> Method for detecting a specific protein or message. A spot of solution is dotted onto nitrocellulose paper, a specific antibody or probe is allowed to bind and the presence of bound antibody/probe then shown by using a peroxidase coupled second antibody, as in Western blotting or by other visualisation methods. (18 Nov 1997) |
| Southern blot technique |
a technique for transferring DNA fragments separated by agarose gel electrophoresis to a nitrocellulose filter, on which specific fragments can then be detected by their hybridization to probes, which were labeled radioactively in the original technique but are now often labeled using nonradioactive methods.
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| Southern blot technique (blot analysis, blot hybridization, blot test) |
see under technique.
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제품명 |
판매사 |
보험코드 | 성분/함량 | 구분/보험급여 |
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제품명 |
판매사 |
보험코드 | 성분/함량 | 구분/보험급여 |
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