| SCPNT | Southern California Postrotary Nystagmus Test |
|---|---|
| SCSIT | Southern California Sensory Integration Test |
| STSA | Southern Thoracic Surgical Association |
| RIBA | Recombinant Immuno-Blot Assay |
| BLOT | British Library of Tape |
| SB | Southern Blot |
|---|---|
| SBH | Southern blot hybridisation |
| ENSO | El Nino Southern Oscillation |
| SBMV | Southern bean mosaic virus |
| SB | Southern blotting |
| blot, southern | A common test for checking for a match between DNA molecules. DNA fragments are separated by agarose gel electrophoresis, transferred (blotted) onto membrane filters, and hybridised with complementary radiolabelled probes. The aim is to detect specific base sequenceswith the probes. Lest all of this sound esoteric, note that in the television series The X Files a Southern blot was done (in this case, to learn if some alien virus genome had been integrated into a person's genome). The Southern blot is named after its inventor, the British biologist M.E. Southern. There is also a Northern blot and a Western blot. (12 Dec 1998) |
|---|---|
| southern blot | A technique used for searching for a specific DNA fragment. The process is as follows: 1. separate DNA fragments by gel electrophoresis 2. Change pH of gel to basic, thus allowing disruption of H-bonds 3. blot gel with nitrocellulose paper 4. Heat paper so as to fix DNA fragments 5. probe with labelled messenger RNA or cDNA 6. wash Complementary mRNA/cDNA fragments will have hybridised. (09 Oct 1997) |
| Southern blot analysis | A procedure to separate and identify DNA sequences; DNA fragments are separated by electrophoresis on an agarose gel, transferred (blotted) onto a nitrocellulose or nylon membrane, and hybridised with complementary (labelled) nucleic acid probes. (05 Mar 2000) |
| africa, southern | The geographical area of africa comprising angola, botswana, lesotho, malawi, mozambique, namibia, south africa, swaziland, zambia, and zimbabwe. It includes what was formerly called south-west africa or german southwest africa but it was terminated in 1966 by a united nations resolution. (12 Dec 1998) |
| blotting, southern | A method (first developed by e.m. Southern) for detection of DNA that has been electrophoretically separated and immobilised by blotting on nitrocellulose or other type of paper or nylon membrane. (12 Dec 1998) |
| Southern | M.E., 20th century British biologist. See: Southern blot analysis. (05 Mar 2000) |
| Southern blotting | <molecular biology, procedure> General term for the transfer of protein, RNA or DNA molecules from a relatively thick acrylamide or agarose gel or to a paper like membrane (usually nylon or nitrocellulose) by capilliarity or an electric field, preserving the spatial arrangment. Once on the membrane, the molecules are immobilised, typically by baking or by ultra violet irradiation and can then be detected at high sensitivity by hybridisation (in the case of DNA & RNA) or antibody labelling (in the case of protein). RNA blots are called Northern blots, DNA blots, Southern, protein blots, Western. (18 Nov 1997) |
| cell hybridization | Fusion of two or more dissimilar cells, leading to formation of a synkaryon. (05 Mar 2000) |
| cross hybridization | Annealing of a DNA probe to an imperfectly matching DNA molecule. (05 Mar 2000) |
| protein hybridization | The formation of a protein consisting of two or more polypeptide chains from separate and different polypeptide chains. (12 Dec 1998) |
| hybridization | <molecular biology> The process of joining two complementary strands of DNA or one each of DNA and RNA to form a double-stranded molecule. Technique in which single stranded nucleic acids are allowed to interact so that complexes or hybrids, are formed by molecules with sufficiently similar, complementary sequences. By this means the degree of sequence identity can be assessed and specific sequences detected. The hybridisation can be carried out in solution or with one component immobilised on a gel or, most commonly, nitrocellulose paper. Hybrids are detected by various means: visualisation in the electron microscope, by radioactively labelling one component and removing noncomplexed DNA or by washing or digestion with an enzyme that attacks single stranded nucleic acids and finally estimating the radioactivity bound. Hybridisations are done in all combinations: DNA DNA (DNA can be rendered single stranded by heat denaturation), DNA RNA or RNA RNA. In situ hybridisations involve hybridising a labelled nucleic acid (often labelled with a fluorescent dye) to suitably prepared cells or histological sections. This is used particularly to look for specific transcription or localisation of genes to specific chromosomes (FISH analysis). <zoology> The mating of individuals from different species or sub-species. (13 Oct 1997) |
| somatic cell hybridization | Production of a heterokaryon. (05 Mar 2000) |
| nucleic acid hybridization | Widely used technique which exploits the ability of complementary sequences in single-stranded dnas or rnas to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (kendrew, encyclopedia of molecular biology, 1994, p503; dorlands, 28th ed, p781) (12 Dec 1998) |
| DNA hybridization | A technique used to determine the relatedness of microorganisms by the speed and efficiency of the reassociation of single-stranded DNA to form double-stranded DNA when one of the strands originates from one organism and the other strand from another organism; occurs when the base sequences are complementary or nearly so. (05 Mar 2000) |
| in situ hybridization | <molecular biology> The use of a DNA or RNA probe to detect the complementary DNA sequence. (12 Dec 1998) |
제품명 |
판매사 |
보험코드 | 성분/함량 | 구분/보험급여 |
|---|
제품명 |
판매사 |
보험코드 | 성분/함량 | 구분/보험급여 |
|---|