| AVS | aortic valve stenosis; arteriovenous shunt; auditory vocal sequencing |
|---|---|
| GAWTS | genomic amplification with transcript sequencing |
| RAWTS | ribonucleic acid amplification with transcript sequencing |
| VMST | visual motor sequencing test |
| RNA | Ribo-Nucleic Acid |
| SBT | Sequencing Based Typing |
|---|---|
| SBR | Sequencing Batch Reactor |
| SBH | Sequencing by Hybridization |
| D RNA | defective RNA |
| HCV RNA | Hepatitis C virus RNA |
| gene sequencing | Determination of the sequence of nucleotide bases in a strand of DNA. (14 Nov 1997) |
|---|---|
| maxam-gilbert sequencing | A lab technique used to find out the sequence of nucleotide bases in a nucleic acid (a DNA or RNA molecule). The technique involves putting copies of the nucleic acid into separate test tubes, each of which contains a chemical that will cleave the molecule at a different base (either adenine, guanine, cytosine, or thymine or uracil (the last depending on whether it is DNA or RNA)). The result is that each of the test tubes contains fragments of the nucleic acid that all end at the same base, but at different points on the molecule where the base occurs. The contents of the test tubes are then separated by size with gel electrophoresis (one gel well per test tube, four total wells), the smallest fragments will travel the farthest and the largest will travel the least far from the well. The sequence can then be determined from the picture of the finished gel by noting the sequence of the marks on the gel and from which well they came from. (09 Oct 1997) |
| Maxim-Gilbert sequencing | <molecular biology> A method of sequencing DNA using dimethyl sulfate and hydrazinolysis. (05 Mar 2000) |
| chemical sequencing | A lab technique used to determine the sequence of nucleotides in a DNA molecule. The DNA molecule is labelled with radioactive phosphorous (chemical element P), cut into fragments, and analysed through electrophoresis. (09 Oct 1997) |
| protein sequencing | Determining the sequence of amino acids in a protein, a process carried out by automated techniques. (14 Nov 1997) |
| sequencing | <molecular biology, procedure> Determination of the order of nucleotides (base sequences) in a DNA or RNA molecule or the order of amino acids in a protein. (11 Jun 1998) |
| dideoxy sequencing | The most popular method of DNA sequence determination (c.f. Maxam Gilbert sequencing). Starting with single stranded template DNA, a short complementary primer is annealed and extended by a DNA polymerase. The reaction is split into 4 tubes (called A, C, G or T) each containing a low concentration of the indicated dideoxy nucleotide, in addition to the normal deoxynucleotides. Dideoxynucleotides, once incorporated, block further chain extension and so each tube accumulates a mixture of chains of lengths determined by the template sequence. The 4 reactions are denatured and run out on an acrylamide sequencing gel in neighbouring lanes and the sequence read up the gel according to the order of the bands. (18 Nov 1997) |
| DNA sequencing | <molecular biology> Any lab technique used to find out the sequence of nucleotide bases in a DNA molecule or fragment. Examples are dideoxy sequencing and Maxam-Gilbert sequencing. (09 Oct 1997) |
| acceptor RNA | rNA |
| antisense RNA | <molecular biology> A complementary RNA sequence that binds to (and thus blocks the transcription of) a naturally-occuring (sense) messenger RNA molecule. These proteins can be used to selectively turn off production of certain proteins or block viral genetic instructions, by marking them for destruction by cellular enzymes, in order to prevent the building of new virus or the infection of new cells. (09 Oct 1997) |
| bacteriophage T3 RNA polymerase | <enzyme> Used for the rapid generation of strand-specific RNA molecules that can be used for the identification of genes in hybridization experiments Registry number: EC 2.7.7.- Synonym: t3 RNA polymerase (26 Jun 1999) |
| cap II RNA(nucleoside-2'-)methyltransferase | <enzyme> Converts cap i-terminated mRNA to cap II-terminated mRNA Registry number: EC 2.1.1.- Synonym: cap II methylase (26 Jun 1999) |
| cap I RNA (nucleoside-2'-)methyltransferase | <enzyme> Converts cap 0-terminated mRNA to cap i-terminated mRNA Registry number: EC 2.1.1.- Synonym: cap I methylase (26 Jun 1999) |
| p68 RNA helicase | <enzyme> An RNA helicase isolated from uv-induced tumours in mice; amino acid sequence has been determined Registry number: EC 2.7.7.- Synonym: dead box helicase p68 (26 Jun 1999) |
| masked messenger RNA | <molecular biology> Long lived and stable mRNA found originally in the oocytes of echinoderms and constituting a store of maternal information for protein synthesis that is unmasked (derepressed) during the early stages of morphogenesis. In these early stages the rate of cell division is so rapid that transcription from the embryonic genome cannot occur. Undoubtedly not restricted to oocytes and the term can be applied to any mRNA which is present in inactive form. (18 Nov 1997) |
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