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  • endpoint PCR
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  • PCR(polymerase chain reaction)
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  • polymerase chain reaction (PCR)
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  • polymerase chain reaction(PCR)
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  • polymerase chain reaction=PCR
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  • sib-pair methods
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  • polymerase chain reaction (PCR)
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  • PCr
    (å²) Phosphocreatine
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appl appliance; application, applied
ECVAM European Centre for the Validation of Alternative Methods
FENa, FeNa Fractional Excretion of Sodium
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PCR Polymerase Chain Reaction
Pcr Plasma Creatinine
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AP-PCR Arbitrarily Primed PCR
DOP-PCR Degenerate Oligonucleotide Primed PCR
DDRT-PCR Differential display reverse transcription PCR
IS-PCR In situ PCR
LM-PCR Ligation mediated-PCR
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  • JrId: 2311
    JournalTitle: PCR methods and applications.
    MedAbbr: PCR Methods Appl
    ISSN: 1054-9803
    ESSN:
    IsoAbbr: PCR Methods Appl.
    NlmId: 9201445
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PCR <molecular biology, technique> The first practical system for in vitro amplification of DNA and as such one of the most important recent developments in molecular biology.
Two synthetic oligonucleotide primers, which are complementary to two regions of the target DNA (one for each strand) to be amplified, are added to the target DNA (that need not be pure), in the presence of excess deoxynucleotides and Taq polymerase, a heat stable DNA polymerase. In a series (typically 30) of temperature cycles, the target DNA is repeatedly denatured (around 90_C), annealed to the primers (typically at 50-60_C) and a daughter strand extended from the primers (72_C). As the daughter strands themselves act as templates for subsequent cycles, DNA fragments matching both primers are amplified exponentially, rather than linearly.
The original DNA need thus be neither pure nor abundant and the polymerase chain reaction has accordingly become widely used not only in research, but in clinical diagnostics and forensic science.
Acronym: PCR
(14 Oct 1997)
PCR test <investigation> A very sensitive test that measures the presence or amount of RNA or DNA of a specific organism or virus (for example: HIV or CMV) in the blood or tissue. PCR tests such as HoffmannLa Roche's HIV RT PCR are being used to gauge HIV disease progression and the effect of particular treatments on HIV infection.
(09 Oct 1997)
reverse transcriptase PCR A technique used to amplify RNA targets. The specimen containing the target RNA (e.g., HIV-1 RNA, Hepatitis C Virus RNA) is subjected to reverse transcription to make complementary DNA (cDNA), which is then, in turn, amplified by PCR.
Acronym: RT-PCR
(05 Mar 2000)
differential display PCR Method of image formation in the light microscope based on the method proposed by Nomarski (though strictly speaking all forms of optical microscopy rely to a greater or lesser extent on differential interference). The light beam is split by a Wollaston prism in the condenser, to form slightly divergent beams polarized at right angles. One passes through the specimen (and is retarded if the refractive index is greater) and one through the background nearby: the two are recombined in a second Wollaston prism in the objective and interfere to form an image. The image is spuriously three dimensional the nucleus, for example: appears to stand out above the cell (or be hollowed out) because it has a higher refractive index than the cytoplasm. The Nomarski system has the advantage that there is no phase halo, but the contrast is low and image formation with crowded cells is poor because the background does not differ from the specimen.
(18 Nov 1997)
replica methods <technique> Methods in the preparation of specimens for transmission electron microscopy. The specimen (for example: a piece of freeze fractured tissue) is shadowed with metal and coated with carbon and then the tissue is digested away. The replica is then picked up on a grid and it is the replica that is examined in the microscope.
(18 Nov 1997)
communication methods, total Utilization of all available receptive and expressive modes for the purpose of achieving communication with the hearing impaired, such as gestures, postures, facial expression, types of voice, formal speech and non-speech systems, and simultaneous communication.
(12 Dec 1998)
Crede's methods Instillation of one drop of a 2% solution of silver nitrate into each eye of the newborn infant, to prevent ophthalmia neonatorum, resting the hand on the fundus uteri from the moment of the expulsion of the foetus, and gently rubbing in case of haemorrhage or failing contraction; then, when the afterbirth is loosened it is expelled by firm compression or squeezing of the fundus by the hand, use of manual pressure on a bladder, particularly a paralysed bladder, to express urine.
Synonym: Crede's manoeuvres.
(05 Mar 2000)
seroepidemiologic methods Epidemiologic studies based on the detection through serological testing of characteristic change in the serum level of specific antibodies. Latent subclinical infections and carrier states can thus be detected in addition to clinically overt cases.
(12 Dec 1998)
feeding methods Methods of giving food to humans or animals.
(12 Dec 1998)
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