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AFA acromegaloid facial syndrome; advanced first aid; alcohol-formaldehyde-acetic [fixative]
orth, ortho orthopedics, orthopedic
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Orth's fixative Formalin added to Muller's fixative, used for bringing out chromaffin, studying early degenerative processes and necrosis, and for demonstrating rickettsiae and bacteria.
(05 Mar 2000)
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Orth, Johannes <person> German pathologist, 1847-1923.
See: Orth's fixative, Orth's stain.
(05 Mar 2000)
Orth's stain <technique> A lithium carmine stain for nerve cells and their processes.
(05 Mar 2000)
acetone fixative Acetone used at low temperatures to fix enzymes, particularly phosphatases; it removes fat and glycogen.
(05 Mar 2000)
Altmann's fixative A bichromate-osmic acid fixative.
(05 Mar 2000)
Bouin's fixative A solution of glacial acetic acid, formalin, and picric acid, useful for soft and delicate tissues (as those of embryos) and small pieces of tissues; it preserves glycogen and nuclei and permits brilliant staining, but penetrates slowly, distorts kidney tissue and mitochondria, and does not permit Feulgen stain for DNA.
(05 Mar 2000)
Carnoy's fixative Ethanol, chloroform, and acetic acid (6:3:1) or ethanol and acetic acid (3:1), an extremely rapid fixative used for glycogen preservation and as a nuclear fixative.
(05 Mar 2000)
Marchi's fixative A mixture of Muller's fixative with osmium tetroxide, with potassium chlorate substituted for the potassium dichromate of Muller's fixative for better results; used to demonstrate degenerating myelin.
See: Marchi's stain.
(05 Mar 2000)
Park-Williams fixative A fixative for spirochetes, comprised of a 2% solution of osmic acid to the fumes of which the bacteria are exposed for a few seconds.
(05 Mar 2000)
Regaud's fixative A fixative containing formaldehyde and sodium dichromate, used to preserve mitochondria but not fat; requires afterchroming and extensive washing.
(05 Mar 2000)
glutaraldehyde fixative A fixative used in phosphate or cacodylate buffer for electron microscopy, and as a chromatin and enzyme fixative; may be used preceding osmic acid as a second fixative to add membrane preservation for electron microscopy.
(05 Mar 2000)
Golgi's osmiobichromate fixative <chemical> An osmic-bichromate mixture used to demonstrate nerve cells and their processes.
(05 Mar 2000)
Champy's fixative A mixture of potassium bichromate, chromic acid, and osmic acid, considered an excellent cytologic fixative with advantages and disadvantages similar to those of Flemming's fixative; it differs from Flemming's fixative in substituting bichromate for acetic acid.
(05 Mar 2000)
methanol fixative A fixative used with dry blood films, and often incorporated into the stain used.
(05 Mar 2000)
picroformol fixative A fixative containing formalin and picric acid.
(05 Mar 2000)
Muller's fixative A hardening fixative composed of potassium dichromate, sodium sulfate, and distilled water, similar to Regaud's fixative.
(05 Mar 2000)
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