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SPF skin protection factor; specific-pathogen free; spectrophotofluorometer; S-phase fraction; split pro...
APIP additional personal injury protection
CPC central posterior curve; cerebellar Purkinje cell; cerebral palsy clinic; cerebral performance categ...
CPT carnitine palmityl transferase; carotid pulse tracing; chest physiotherapy; child protection team; c...
EP echo planar; ectopic pregnancy; edible portion; electrophoresis; electrophysiologic; electroprecipit...
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MNase Micrococcal nuclease
SNase Staphylococcal Nuclease
Nase nuclease
DAS-ELISA Double antibody sandwich enzyme-linked immunosorbent assays
EMSA Electromobility shift assays
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CancerWEB ¿µ¿µ ÀÇÇлçÀü À¯»ç °Ë»ö °á°ú : 15 ÆäÀÌÁö: 1
chemiluminescence assays Including a subcategory using bioluminescence (biologically derived chemiluminescence agents), use the generation of light from oxidative chemical reactions as an indicator of the quantity of unbound luminescent labelled antigen. This allows quantitation of unlabelled antigen from patient specimens in a variety of homogeneous (single phase) or heterogeneous (multiple phase) immunoassay techniques.
(05 Mar 2000)
drug screening assays, antitumour Methods of investigating the effectiveness of anticancer cytotoxic drugs and biologic inhibitors. These include in vitro cell-kill models and cytostatic dye exclusion tests as well as in vivo measurement of tumour growth parameters in laboratory animals.
(12 Dec 1998)
browse protection <ecology> Mechanical or chemical protection of shrubs and trees against damage caused by game and grasing animals.
(09 Oct 1997)
cathodic protection <chemistry> The attachment of an active metal to a system to protect the system from corrosion. The active metal serves as the anode, making the metal in the system a cathode.
(16 Mar 1998)
protection Synonym: protective block.
Origin: see protective
(05 Mar 2000)
protection test A test to determine the antimicrobial activity of a serum by inoculating a susceptible animal with a mixture of the serum and the virus or other microbe being tested.
Synonym: neutralization test.
(05 Mar 2000)
sun protection factor A number on a scale (from 2 upwards) for rating sunscreens. Sunscreens with an spt of 15 or higher provide the best protection from the sun's harmful rays.
(12 Dec 1998)
ecological protection <ecology> Protection consists of measures undertaken to protect and preserve elements of an ecosystem's structure and functions against future degradation.
(10 Nov 1998)
environmental protection agency <organisation> The U.S. Regulatory agency for biotechnology of microbes. The major lawsunder which the agency has regulatory powers are the Federal Insecticide, Fungicide, and Rodenticide act (FIFRA), and the Toxic Substances ControlAct (TSCA).
(30 Mar 1998)
united states environmental protection agency An agency in the executive branch which endeavors to abate and control pollution in the areas of air, water, solid waste, noise, radiation, and toxic substances.
(12 Dec 1998)
APEX nuclease <enzyme> DNA repair enzyme having both exonuclease and apurinic-apyrimidinic endonuclease activities; apex is from mouse; rapen from rat has high homology with apex
Registry number: EC 3.1.-
Synonym: rapen redox factor
(26 Jun 1999)
aspergillus nuclease s1 <enzyme> An enzyme that catalyses endonucleolytic cleavage to 5-phosphomononucleotides and 5-phosphooligonucleotide end-products. It has a preference for single-stranded substrates but is active with either ribo- or deoxyribonucleic acids.
Registry number: EC 3.1.30.1
(12 Dec 1998)
viral alkaline nuclease <enzyme> Has both exo and endonuclease activity
Registry number: EC 3.1.-
Synonym: hsv type 1 alkaline nuclease, herpes simplex virus type I alkaline nuclease, alkaline nuclease, herpes simplex virus
(26 Jun 1999)
repair nuclease <enzyme, molecular biology> Class of enzymes involved in DNA repair. It includes endonucleases that recognise a site of damage or an incorrect base pairing and cut it out and exonucleases that remove neighbouring nucleotides on one strand. These are then replaced by a DNA polymerase.
(18 Nov 1997)
restriction nuclease <enzyme, molecular biology> Class of bacterial enzymes that cut DNA at specific sites. In bacteria their function is to destroy foreign DNA, such as that of bacteriophages (host DNA is specifically modified at these sites).
Type I restriction endonucleases occur as a complex with the methylase and a polypeptide that binds to the recognition site on DNA. They are often not very specific and cut at a remote site.
Type II restriction endonucleases are the classic experimental tools. They have very specific recognition and cutting sites. The recognition sites are short, 4-8 nucleotides and are usually palindromic sequences. Because both strands have the same sequence running in opposite directions the enzymes make double stranded breaks, which, if the site of cleavage is off centre, generates fragments with short single stranded tails, these can hybridise to the tails of other fragments and are called sticky ends.
They are generally named according to the bacterium from which they were isolated (first letter of genus name and the first two letters of the specific name). The bacterial strain is identified next and multiple enzymes are given Roman numerals. For example the two enzymes isolated from the R strain of E. Coli are designated Eco RI and Eco RII.
(10 Mar 1998)
MeSH(Medical Subject Headings) ¸ÂÃã °Ë»ö (http://www.nlm.nih.gov) °á°ú : 1 ÆäÀÌÁö: 1
  • Nuclease Protection Assays - »õâ Techniques for measuring specific nucleic acid interaction with another nucleic acid or with a protein by digestion of the non-interacting nucleic acid by various nucleases. After all non-interacting regions are eliminated by nuclease digestion, the protected nucleic acid that remains is analyzed. DNA FOOTPRINTING utilizes this technique to analyze the DNA contact sites of DNA-BINDING PROTEINS.
    Synonyms : Assay, Nuclease Protection, Assays, Nuclease Protection, Nuclease Protection Assay
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