| NRRL | Northern Regional Research Laboratory |
|---|---|
| FISH | Fluorescence In Situ Hybridization |
| CISH | competitive in situ hybridization |
| FISH | fluorescence in situ hybridization |
| FLASH | fast low angle shot; fluorescence in situ hybridization |
| NT | Northern Territory |
|---|---|
| NFM | Northern fowl mites |
| CGH | Comparative genome hybridization |
| FISH | Filter In Situ Hybridization |
| FISH | Fluorescence In Situ Hybridization |
| africa, northern | The geographical area of africa comprising algeria, egypt, libya, morocco, and tunisia. It includes also the vast deserts and oases of the sahara. It is often referred to as north africa, french-speaking africa, or the magreb. (12 Dec 1998) |
|---|---|
| blot, northern | A technique in molecular biology, used mainly to separate and identify pieces of RNA. Called a Northern blot only because it is similar to a Southern blot (which is named after its inventor, the British biologist M.E. Southern). (12 Dec 1998) |
| blotting, northern | Detection of RNA that has been electrophoretically separated and immobilised by blotting on nitrocellulose or other type of paper or nylon membrane. (12 Dec 1998) |
| northern | 1. Of or pertaining to the north; being in the north, or nearer to that point than to the east or west. 2. In a direction toward the north; as, to steer a northern course; coming from the north; as, a northern wind. Northern diver. <zoology> See Loon. Northern lights. See Aurora borealis, under Aurora. <botany> Northern spy, an excellent American apple, of a yellowish colour, marked with red. Origin: AS. Northerne. Source: Websters Dictionary (01 Mar 1998) |
| Northern blot | <molecular biology, procedure> An electroblotting method in which RNA is transferred to a filter and detected by hybridisation to (32)P labelled RNA or DNA. (18 Nov 1997) |
| Northern blot analysis | A procedure similar to the Southern blot analysis, used mostly to separate and identify RNA fragments; typically via transferring RNA fragments from an agarose gel to a nitrocellulose filter followed by detection with a suitable probe. Origin: coined to distinguish it from eponymic Southern blot a. (05 Mar 2000) |
| northern territory | A territory in north central Australia, east of the state of queensland. Its capital is darwin. Originally a part of new south wales, it was annexed to south Australia in 1863, entered the commonwealth as part of it in 190l, transferred to the commonwealth in 1911, divided in 1927, but was reestablished in 1931. It was granted self government within the commonwealth of Australia in 1978. (12 Dec 1998) |
| cell hybridization | Fusion of two or more dissimilar cells, leading to formation of a synkaryon. (05 Mar 2000) |
| cross hybridization | Annealing of a DNA probe to an imperfectly matching DNA molecule. (05 Mar 2000) |
| protein hybridization | The formation of a protein consisting of two or more polypeptide chains from separate and different polypeptide chains. (12 Dec 1998) |
| hybridization | <molecular biology> The process of joining two complementary strands of DNA or one each of DNA and RNA to form a double-stranded molecule. Technique in which single stranded nucleic acids are allowed to interact so that complexes or hybrids, are formed by molecules with sufficiently similar, complementary sequences. By this means the degree of sequence identity can be assessed and specific sequences detected. The hybridisation can be carried out in solution or with one component immobilised on a gel or, most commonly, nitrocellulose paper. Hybrids are detected by various means: visualisation in the electron microscope, by radioactively labelling one component and removing noncomplexed DNA or by washing or digestion with an enzyme that attacks single stranded nucleic acids and finally estimating the radioactivity bound. Hybridisations are done in all combinations: DNA DNA (DNA can be rendered single stranded by heat denaturation), DNA RNA or RNA RNA. In situ hybridisations involve hybridising a labelled nucleic acid (often labelled with a fluorescent dye) to suitably prepared cells or histological sections. This is used particularly to look for specific transcription or localisation of genes to specific chromosomes (FISH analysis). <zoology> The mating of individuals from different species or sub-species. (13 Oct 1997) |
| somatic cell hybridization | Production of a heterokaryon. (05 Mar 2000) |
| nucleic acid hybridization | Widely used technique which exploits the ability of complementary sequences in single-stranded dnas or rnas to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (kendrew, encyclopedia of molecular biology, 1994, p503; dorlands, 28th ed, p781) (12 Dec 1998) |
| DNA hybridization | A technique used to determine the relatedness of microorganisms by the speed and efficiency of the reassociation of single-stranded DNA to form double-stranded DNA when one of the strands originates from one organism and the other strand from another organism; occurs when the base sequences are complementary or nearly so. (05 Mar 2000) |
| in situ hybridization | <molecular biology> The use of a DNA or RNA probe to detect the complementary DNA sequence. (12 Dec 1998) |
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