| WAR | Wasserman antigen reaction; without additional reagents |
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| FA | false aneurysm; Families Anonymous; Fanconi anemia; far advanced; fatty acid; febrile antigen; femor... |
| FAT | family attitudes test; fluorescent antibody technique; fluorescent antibody test |
| FTA | fluorescent titer antibody; fluorescent treponemal antibody |
| IFA | idiopathic fibrosing alveolitis; immunofluorescence assay; immunofluorescent antibody; incomplete Fr... |
| AB-PAS | Alcian blue-periodic acid Schiff |
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| PAS | Periodic Acid Schiff |
| PAS | Periodic Acid-Schiff reaction |
| PAS | Periodic acid-Schiff reagent |
| SB | Schiff base |
| Kasten's fluorescent Schiff reagents | Fluorescent analogues of Schiff's reagent which are fluorescent basic dyes lacking acidic side groups and containing one or more primary amine groups; used in cytochemical detection of DNA in Kasten's fluorescent Feulgen stain, polysaccharides in Kasten's fluorescent PAS stain, and proteins in the ninhydrin-Schiff stain; such analogues include acriflavine, auramine O, and flavophosphine N. (05 Mar 2000) |
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| Kasten's fluorescent Feulgen stain | <technique> A fluorescent modification of the Feulgen stain, utilizing any one of a variety of fluorescent basic dyes to which SO2 is added; the brilliant fluorescence makes this method unusually sensitive and adaptable to cytofluorometric quantification of DNA. (05 Mar 2000) |
| Kasten's fluorescent PAS stain | <technique> A fluorescent modification of the periodic acid Schiff stain for polysaccharides which uses one of Kasten's fluorescent Schiff reagents. (05 Mar 2000) |
| sulfhydryl reagents | Chemical agents that react with sh groups. This is a chemically diverse group that is used for a variety of purposes. Among these are enzyme inhibition, enzyme reactivation or protection, and labelling. (12 Dec 1998) |
| indicators and reagents | Substances used for the detection, identification, analysis, etc. Of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., colour, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidisers, reducers, fluxes, and colourimetric reagents. (12 Dec 1998) |
| Kasten, Frederick | <person> U.S. Histochemist and cell biologist, *1927. See: Kasten's fluorescent Schiff reagents, Kasten's fluorescent Feulgen stain, Kasten's fluorescent PAS stain. (05 Mar 2000) |
| periodic acid Schiff reaction | <technique> A histochemical technique based on periodic acid oxidation of a substance containing the 1,2-glycol grouping. It is used for staining carbohydrates as the resulting dialdehyde reacts with Schiff reagent to form a coloured product. The adjacent hydroxyl groups are oxidized to form aldehydes by periodic acid (HIO4) and these aldehyde groups react with Schiff's reagent (basic fuchsin decolourised by sulphurous acid) to give a purple colour. Acronym: PAS (18 Nov 1997) |
| periodic acid Schiff stain | <technique> A histochemical technique based on periodic acid oxidation of a substance containing the 1,2-glycol grouping. It is used for staining carbohydrates as the resulting dialdehyde reacts with Schiff reagent to form a coloured product. Substances that can be demonstrated include carbohydrates, mucins, cartilage matrix, collagen, reticulum, basement membranes, fibrin, thyroid colloid, amyloid, glomerular hyaline deposits, and a number of other secretions or tissue constituents. Also used in for staining gels on which glycoproteins have been run. See: periodic acid Schiff reaction Synonym: PAS stain. (22 Sep 2002) |
| Schiff base | The reaction of a primary amine with an aldehyde or ketone yields an imine sometimes called a Schiff base. When an arylamine is used the Schiff base may form an intermediate in a staining reaction, for example for polysaccharides. (18 Nov 1997) |
| schiff bases | Condensation products of aromatic amines and aldehydes forming azomethines substituted on the n atom, containing the general formula r-n:chr. (12 Dec 1998) |
| Schiff, Hugo | <person> German chemist in Florence, 1834-1915. See: Schiff base, Schiff's reagent, Kasten's fluorescent Schiff reagents, periodic acid-Schiff stain, ninhydrin-Schiff stain for proteins. (05 Mar 2000) |
| Schiff, Moritz | <person> German physiologist, 1823-1896. See: Schiff-Sherrington phenomenon. (05 Mar 2000) |
| Schiff-Sherrington phenomenon | When the spinal cord is transected in the midthoracic region or a little lower, the stretch and other postural reflexes of the upper extremity become exaggerated; if the transection is made in the sacral cord, a similar effect is observed in the lower limbs. The effect is regarded as a release phenomenon, i.e., release from an inhibitory influence normally exerted by the spinal segments below the transection. (05 Mar 2000) |
| Schiff's reagent | An aqueous solution of basic fuchsin or pararosaniline which is decolorised by sulfur dioxide, commonly prepared by addition of hydrochloric acid to a dye solution containing a metabisulphite or bisulphite salt; used for aldehydes and in histochemistry to detect polysaccharides, DNA, and proteins. See: Feulgen stain, periodic acid-Schiff stain, ninhydrin-Schiff stain for proteins. (05 Mar 2000) |
| ninhydrin-Schiff stain | <technique> For proteins, proteins are revealed by using ninhydrin or alloxan to produce aldehydes from primary aliphatic amines by oxidative deamination; the aldehydes are shown by reaction with Schiff's reagent. (05 Mar 2000) |
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