| 영문 | Gram stain | 한글 | 그람염색 |
|---|---|---|---|
| 설명 | 미생물을 염색하는 방법 중의 하나. 1884년 덴마크의 의사 H.C.J. 그람(1853~ 1938)이 고안한 특수 염색법이다. 이 염색법을 사용하면 미생물은 양성균과 음성균의 2무리로 크게 나뉜다. 염색법은 균을 유리면에 바르고 가열 고정한다. 젠시안바이오렛, 크리스탈 바이오렛, 메틸바이오렛 등 파라로즈 아닐린계 색소의 어느 하나를 사용하여 우선 염색하고 이어서 루골액을 작용시킨 뒤, 에탄올 또는 아세톤으로 탈색한다. 이때 탈색되지 않고 보라색을 타나내는 것을 그람양성균이라 부르며, 포도알균-폐렴알균-사슬알균 등이 대표적인 예이다. 탈색되면 그람음성균이라 부르며, 대장균-녹농균 등이 있다. 최후에 사프라닌, 푹신 등 적색계의 색소액으로 후염색을 한다. 미생물의 감별에 중요한 염색법이며 염색성의 차이는 영양요구성, 화학요법제(항생물질 등)에 대한 감수성의 차이와도 관련되는 점이 많으므로 사용약제의 선택시에도 중요한 역할을 한다. 그람양성균과 음성균의 차이는 세균벽의 구조에 있는데, 양성균은 세포막의 바깥쪽 펩티드글리칸이라는 당단백질층의 두께가 20~80nm나 되고, 그램음성균의 펩티드글리칸 층은 2~3nm로 얇고, 다시 바깥쪽에 외막이라 부르는 층이 있다. |
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| GMS | General Medical Service; geriatric mental state; Gilbert-Meulengracht syndrome; Gomori methenamine s... |
|---|---|
| AF | abnormal frequency; acid-fast; adult female; afebrile; aflatoxin; albumin-free; albumose-free; aldeh... |
| TAF | albumose-free tuberculin [Ger. Tuberculin Albumose frei]; tissue angiogenesis factor; toxin-antitoxi... |
| AFB Stain | Acid Fast Bacilli Stain |
| HE stain | Hematoxylin-Eosin stain |
| AF | Aldehyde Fuchsin |
|---|---|
| GMS | Gomori Methenamine-Silver |
| PWS | Port Wine Stain |
| HBFP | Haematoxylin--Basic Fuchsin--picric acid |
| ALDH | Aldehyde dehydrogenase |
| Gomori's aldehyde fuchsin stain | <technique> A stain used to demonstrate beta cells of the pancreas, storage form of thyrotrophic hormone in beta cells of the anterior pituitary, hypophyseal neurosecretory substance, mast cells, granules, elastic fibres, sulfated mucins, and gastric chief cells. (05 Mar 2000) |
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| aldehyde fuchsin | A stain developed by Gomori, utilizing basic fuchsin paraldehyde and hydrochloric acid; it produces violet staining of elastic fibres, mast cell granules, gastric chief cells, beta cells of the pancreatic islets, and certain hypophyseal beta granules; other pituitary granules and cells stain in other colours. See: Gomori's aldehyde fuchsin stain. (05 Mar 2000) |
|---|---|
| Gomori-Jones periodic acid-methenamine-silver stain | <technique> A staining method using methenamine silver, periodic acid, gold chloride, haematoxylin, and eosin to delineate basement membrane, reticulin, collagen, and nuclei; used in renal histopathology. See: Rambourg's periodic acid-chromic methenamine-silver stain. (05 Mar 2000) |
| Gomori's chrome alum haematoxylin-phloxine stain | <technique> A technique used to demonstrate cytoplasmic granules, after Bouin's or formalin-Zenker fixatives, using oxidised haematoxylin plus phloxine; in the pancreas, beta cells are blue, alpha and delta cells are red, and zymogen granules are red to unstained; in the pituitary, alpha cells are pink, beta cells and chromophobes are gray-blue, and nuclei are purple to blue. (05 Mar 2000) |
| Gomori's methenamine-silver stain | <technique> Techniques for 1) argentaffin cells: a method using a methenamine-silver solution in combination with gold chloride, sodium thiosulphate, and safranin O; argentaffin granules appear brown-black against a green background; 2) urates: warm sections are treated directly with a hot methenamine-silver solution to produce a blackening of urates; 3) fungi: see Grocott-Gomori methenamine-silver stain; 4) melanin, which reduces silver nitrate. (05 Mar 2000) |
| Gomori's non-specific acid phosphatase stain | <technique> A method in which formalin-fixed frozen sections are incubated in a substrate containing sodium beta-glycerophosphate and lead nitrate at pH 5.0; the insoluble lead phosphate produced is treated with ammonium sulfide to give a black lead sulfide. (05 Mar 2000) |
| Gomori's non-specific alkaline phosphatase stain | <technique> A calcium-cobalt sulfide method using frozen sections or cold acetone-or formalin-fixed paraffin sections, plus sodium beta-glycerophosphate as a substrate at pH 9.0 to 9.5 with Mg++ as activator; calcium ions precipitate the liberated phosphate, cobalt salt replaces the calcium phosphate, and ammonium sulfide converts the product to a black cobalt sulfide. (05 Mar 2000) |
| Gomori's one-step trichrome stain | <technique> A connective tissue stain that uses haematoxylin and a dye mixture containing chromotrope 2R and light green or aniline blue; muscle fibres appear red, collagen is green (or blue if aniline blue is used), and nuclei are blue to black. (05 Mar 2000) |
| Gomori's silver impregnation stain | <technique> A reliable method for reticulin, as an aid in the diagnosis of neoplasm and early cirrhosis of the liver; the staining solution employs silver nitrate, potassium hydroxide, and ammonia water carefully prepared to avoid having silver precipitate. (05 Mar 2000) |
| Grocott-Gomori methenamine-silver stain | <technique> A modification of Gomori's methenamine-silver stain for fungi in which sections are pretreated with chromic acid before addition of the methenamine-silver solution and then counterstained with light green to demonstrate black-brown fungi against a pale green background. (05 Mar 2000) |
| Altmann's anilin-acid fuchsin stain | <technique> A mixture of picric acid, anilin, and acid fuchsin which stains mitochondria crimson against a yellow background. (05 Mar 2000) |
| basic fuchsin-methylene blue stain | <technique> A stain for intact epoxy sections; semi-thick sections of plastic-embedded tissues have nuclei stained purple; collagen, elastic lamina, and connective tissue are stained blue; mitochondria, myelin, and lipid droplets are stained red; cytoplasm, smooth muscle cells, axoplasm, and chrondroblasts are stained pink. (05 Mar 2000) |
| haematoxylin-malachite green-basic fuchsin stain | <technique> A stain for epoxy resin-extracted sections; semi-thick sections have their plastic dissolved out and the residual tissue is stained sequentially with the various dyes; nuclei and astrocytes are purplish-pink and myelin, lipid droplets, nucleoli, and oligodendrocytes are bright blue-green. (05 Mar 2000) |
| Gomori, George | <person> Hungarian histochemist in the U.S., 1904-1957. See: Grocott-Gomori methenamine-silver stain, Gomori's non-specific alkaline phosphatase stain, Gomori's one-step trichrome stain, Gomori's silver impregnation stain, Gomori's chrome alum haematoxylin-phloxine stain. See entries under stain. (05 Mar 2000) |
| Gomori procedure | <procedure> Cytochemical staining procedure used to localise acid phosphatases. Depends upon the production of phosphate ions from organic phospho esters such as _ glycerophosphate. The phosphate in the presence of lead ions causes the formation of a precipitate of lead salt that is converted to the brown sulphide of lead by the action of yellow ammonium sulphide. (18 Nov 1997) |
| acid fuchsin | A mixture of the sodium salts bi-and trisulfonic acids of rosanilin and pararosanilin; used as an indicator dye and for staining of cytoplasm and collagen. Synonym: rubin S, rubine. (05 Mar 2000) |
제품명 |
판매사 |
보험코드 | 성분/함량 | 구분/보험급여 |
|---|
제품명 |
판매사 |
보험코드 | 성분/함량 | 구분/보험급여 |
|---|