| CM | California mastitis [test]; calmodulin; capreomycin; carboxymethyl; cardiac murmur; cardiac muscle; ... |
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| FCM | flow cytometry |
| FFC | fixed flexion contracture; fluorescence flow cytometry; free from chlorine |
| IAC | image analysis cytometry; ineffective airway clearance; internal auditory canal; interposed abdomina... |
| FC | Flow Cytometry |
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| FCM | Flow Cytometry |
| FCXM | Flow cytometry crossmatch |
| ICM | Image cytometry |
| LSC | Laser scanning cytometry |
| cytometry | The counting of cells, especially blood cells, using a cytometer or haemocytometer. Flow cytometry, a method of measuring fluorescence from stained cells that are in suspension and flowing through a narrow orifice, usually in combination with one or two lasers to activate the dyes; used to measure cell size, number, viability, and nucleic acid content with the aid of acridine orange, Kasten's fluorescent Feulgen stain, ethidium bromide, trypan blue, and other selected staining reagents. Synonym: flow cytophotometry. (05 Mar 2000) |
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| cytometry, flow | Analysis of biological material by detection of the light-absorbing or fluorescing properties of cells or subcellular fractions such as chromosomes passing in a narrow stream through a laser beam. Flow cytometry is used with automated sorting devices to sort successive droplets of the stream into different fractions depending on the fluorescence emitted by each droplet. (12 Dec 1998) |
| image cytometry | A technique encompassing morphometry, densitometry, neural networks, and expert systems that has numerous clinical and research applications and is particularly useful in anatomic pathology for the study of malignant lesions. The most common current application of image cytometry is for DNA analysis, followed by quantitation of immunohistochemical staining. (12 Dec 1998) |
| flow cytometry | <technique> Flow cytometry is an emerging technique which holds great promise for the separation, classification and quantitation of blood cells and antibodies which affect blood cells. Complex computerised instruments are used to pass a monocellular stream of cells, platelets or other microscopic particulate elements through a beam of laser light. The cells are categorised first by size and then computer analysed to sort the mixture of cellular elements into cell type by size. Cells are labelled with fluorescent dye and then passed, in suspending medium, through a narrow dropping nozzle so that each cell is in a small droplet. A laser based detector system is used to excite fluorescence and droplets with positively fluorescent cells are given an electric charge. Charged and uncharged droplets are separated as they fall between charged plates and so collect in different tubes. The machine can be used either as an analytical tool, counting the number of labelled cells in a population or to separate the cells for subsequent growth of the selected population. Further sophistication can be built into the system by using a second laser system at right angles to the first to look at a second fluorescent label or to gauge cell size on the basis of light scatter. The great strength of the system is that it looks at large numbers of individual cells and makes possible the separation of populations with, for example: particular surface properties. Tabulation of counted data in conjunction with size analysis enables determination of relative percentages of each specific cellular subset for which monoclonal antibody conjugates are utilised, even when the size of the cell is identical to other subset species. Flow cytometry is a slightly imprecise but common term for the use of the Fluorescence-activated Cell Sorter (FACS). (01 Dec 1998) |
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