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Cleland nomenclature A nomenclature for representing the binding mechanisms of enzyme-catalyzed reactions; in this nomenclature, substrates are represented by the letters A, B, C, etc., while products are represented by P, Q, R, etc., enzyme by E, and modified forms of the enzyme by F, G, etc.; in addition, the number of substrates or products is represented by uni, bi, ter, etc.; thus, an aminotransferase reaction (e.g., alanine transaminase) has a ping-pong bi bi mechanism; glutamine synthetase has been reported to have a random ter ter mechanism.subentries under mechanism.
(05 Mar 2000)
Cleland's reagent <chemical> A reagent commonly used in biochemical studies as a protective agent to prevent the oxidation of sh (thiol) groups and for reducing disulphides to dithiols.
Pharmacological action: sulfhydryl reagents, expectorants.
Chemical name: 2,3-Butanediol, 1,4-dimercapto-, (R*,R*)-
(12 Dec 1998)
sulfhydryl reagents Chemical agents that react with sh groups. This is a chemically diverse group that is used for a variety of purposes. Among these are enzyme inhibition, enzyme reactivation or protection, and labelling.
(12 Dec 1998)
indicators and reagents Substances used for the detection, identification, analysis, etc. Of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., colour, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidisers, reducers, fluxes, and colourimetric reagents.
(12 Dec 1998)
Kasten's fluorescent Schiff reagents Fluorescent analogues of Schiff's reagent which are fluorescent basic dyes lacking acidic side groups and containing one or more primary amine groups; used in cytochemical detection of DNA in Kasten's fluorescent Feulgen stain, polysaccharides in Kasten's fluorescent PAS stain, and proteins in the ninhydrin-Schiff stain; such analogues include acriflavine, auramine O, and flavophosphine N.
(05 Mar 2000)
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