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AFA acromegaloid facial syndrome; advanced first aid; alcohol-formaldehyde-acetic [fixative]
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Carnoy's fixative Ethanol, chloroform, and acetic acid (6:3:1) or ethanol and acetic acid (3:1), an extremely rapid fixative used for glycogen preservation and as a nuclear fixative.
(05 Mar 2000)
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Carnoy Jean Baptiste, French biologist, 1836-1899.
See: Carnoy's fixative.
(05 Mar 2000)
acetone fixative Acetone used at low temperatures to fix enzymes, particularly phosphatases; it removes fat and glycogen.
(05 Mar 2000)
Altmann's fixative A bichromate-osmic acid fixative.
(05 Mar 2000)
Bouin's fixative A solution of glacial acetic acid, formalin, and picric acid, useful for soft and delicate tissues (as those of embryos) and small pieces of tissues; it preserves glycogen and nuclei and permits brilliant staining, but penetrates slowly, distorts kidney tissue and mitochondria, and does not permit Feulgen stain for DNA.
(05 Mar 2000)
Marchi's fixative A mixture of Muller's fixative with osmium tetroxide, with potassium chlorate substituted for the potassium dichromate of Muller's fixative for better results; used to demonstrate degenerating myelin.
See: Marchi's stain.
(05 Mar 2000)
Park-Williams fixative A fixative for spirochetes, comprised of a 2% solution of osmic acid to the fumes of which the bacteria are exposed for a few seconds.
(05 Mar 2000)
Regaud's fixative A fixative containing formaldehyde and sodium dichromate, used to preserve mitochondria but not fat; requires afterchroming and extensive washing.
(05 Mar 2000)
glutaraldehyde fixative A fixative used in phosphate or cacodylate buffer for electron microscopy, and as a chromatin and enzyme fixative; may be used preceding osmic acid as a second fixative to add membrane preservation for electron microscopy.
(05 Mar 2000)
Golgi's osmiobichromate fixative <chemical> An osmic-bichromate mixture used to demonstrate nerve cells and their processes.
(05 Mar 2000)
Champy's fixative A mixture of potassium bichromate, chromic acid, and osmic acid, considered an excellent cytologic fixative with advantages and disadvantages similar to those of Flemming's fixative; it differs from Flemming's fixative in substituting bichromate for acetic acid.
(05 Mar 2000)
methanol fixative A fixative used with dry blood films, and often incorporated into the stain used.
(05 Mar 2000)
picroformol fixative A fixative containing formalin and picric acid.
(05 Mar 2000)
Muller's fixative A hardening fixative composed of potassium dichromate, sodium sulfate, and distilled water, similar to Regaud's fixative.
(05 Mar 2000)
Helly's fixative A combination of potassium dichromate, mercuric chloride, formaldehyde, and distilled water, used as a microanatomic fixative for cytoplasmic granules and nuclear staining; has the same disadvantages as Zenker's fixative.
(05 Mar 2000)
Hermann's fixative <chemical> A hardening fixative of glacial acetic acid, osmic acid, and platinum chloride.
(05 Mar 2000)
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