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picrocarmine stain <technique> A red crystalline powder derived from a solution of carmine, ammonia, and picric acid which is evaporated, leaving the powder (soluble in water); it produces excellent staining of keratohyaline granules.
(05 Mar 2000)
picro-Mallory trichrome stain <technique> A modification of Mallory's trichrome stain that involves the addition of picric acid.
(05 Mar 2000)
picronigrosin stain <technique> A solution of nigrosin in picric acid, used for staining connective tissue.
(05 Mar 2000)
Weigert-Gram stain <technique> A stain for bacteria in tissues in which sections are stained in alum-haematoxylin, then in eosin, aniline methyl violet, and Lugol's solution.
(05 Mar 2000)
Weigert's iron haematoxylin stain <technique> A nuclear staining solution containing haematoxylin, ferric chloride, and hydrochloric acid; useful in combination with von Gieson's stain, especially for demonstrating connective tissue elements or Entamoeba histolytica in sections.
(05 Mar 2000)
Weigert's stain <technique> 1. For actinomyces, a staining method using immersion in a dark red orsellin solution in alcohol, then staining in crystal-violet solution.
See: iron haematoxylin.
2. For elastin, a staining solution of fuchsin, resorcin, and ferric chloride; elastic fibres stain blue-black.
3. For fibrin, a staining method using solutions of aniline-crystal violet and iodine-potassium iodide, then decolorizing in aniline oil and xylol; the fibrin is stained dark blue.
4. For myelin, a staining method using ferric chloride and haematoxylin; myelin stains deep blue, degenerated portions a light yellowish colour.
5. For neuroglia, a complicated process in which the final treatment is like that for staining fibrin; neuroglia and nuclei stain blue.
6. For reticulum, a silver impregnation technique in which reticulum appears as black, well-defined fibres without beading and with a relatively clear background.
(05 Mar 2000)
Mowry's colloidal iron stain <technique> A stain used for demonstrating acid mucopolysaccharides.
(05 Mar 2000)
Williams' stain <technique> A stain for Negri bodies which utilises picric acid, fuchsin, and methylene blue; Negri bodies are magenta, granules and nerve cells blue, and erythrocytes yellowish.
(05 Mar 2000)
plasma stain <technique> Plasmatic stainplasmic stain, a stain whose principal affinity is for the cytoplasm of cells.
(05 Mar 2000)
MSB trichrome stain <technique> A stain for fibrin using martius yellow, brilliant crystal scarlet 6R, and soluble blue; fibrin is selectively stained red and connective tissue appears blue.
(05 Mar 2000)
plastic section stain <technique> For electron microscopy, a stain (e.g., osmic acid, PTA, potassium permanganate) used on thin sections of plastic-embedded tissues, utilizing differential attachment of heavy atoms to various cellular and tissue structures so that electrons will be absorbed and scattered by these structures to produce an image; to achieve differential staining, the stain must penetrate nonwettable plastic embedments, for light microscopy, a stain (e.g., alkaline toluidine blue, silver methenamine) used on plastic-embedded tissues to attain higher resolution and more detail than normally possible; semi-thick (0.5-1.5 um) sections are particularly useful in renal pathology, especially in combination with the phase microscope.
(05 Mar 2000)
multiple stain <technique> A mixture of several dyes each having an independent selective action on one or more portions of the tissue.
(05 Mar 2000)
Wright's stain <technique> A staining mixture of eosinates of polychromed methylene blue used in staining of blood smears.
(05 Mar 2000)
contrast stain <technique> A dye used to colour one portion of a tissue or cell which remained unaffected when the other part was stained by a dye of different colour.
Synonym: differential stain.
(05 Mar 2000)
port-wine stain <technique> A mark on the skin that resembles port wine (porto) in its rich ruby red colour. Due to an abnormal aggregation of capillaries, a port-wine stain is a type of haemangioma. It occurs on the face as a sign of sturge-weber syndrome.
(12 Dec 1998)
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