| Glenner-Lillie stain | <technique> For pituitary, a modification of Mann's methyl blue-eosin stain which changes the dye proportions, buffering the dye mixture, and staining at 60캜; basophils are stained blue to black, acidophils are dark red, chromophobe granules are gray to pink, and erythrocytes are orange; with modification, the method is also useful for enterochromaffin cells, goblet cells, Paneth cells, and pancreatic islet cells. (05 Mar 2000) |
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| C-banding stain | <technique> A selective chromosome banding stain used in human cytogenetics, employing Giemsa stain after most of the DNA is denatured or extracted by treatment with alkali, acid, salt, or heat; only heterochromatic regions close to the centromeres and rich in satellite DNA stain, with the exception of the Y chromosome whose long arm usually stains throughout. Synonym: centromere banding stain. (05 Mar 2000) |
| vital stain | <technique> A stain that is taken up by live cells and that can be used to stain, for example: a group of cells in a developing embryo in order to try to determine a fate map. (18 Nov 1997) |
| centromere banding stain | <technique> A selective chromosome banding stain used in human cytogenetics, employing Giemsa stain after most of the DNA is denatured or extracted by treatment with alkali, acid, salt, or heat; only heterochromatic regions close to the centromeres and rich in satellite DNA stain, with the exception of the Y chromosome whose long arm usually stains throughout. Synonym: centromere banding stain. (05 Mar 2000) |
| von Kossa stain | <technique> A stain for calcium in mineralised tissue, utilizing a silver nitrate solution followed by sodium thiosulfate; calcified bone but not osteoid is stained brown to black. Synonym: Kossa stain. (05 Mar 2000) |
| Golgi's stain | <technique> Any of several methods for staining nerve cells, nerve fibres, and neuroglia using fixation and hardening in formalin-osmic-dichromate combinations for various times, followed by impregnation in silver nitrate. (05 Mar 2000) |
| Goodpasture's stain | <technique> A stain for Gram-negative bacteria, using aniline fuchsin. (05 Mar 2000) |
| Gordon and Sweet stain | <technique> A stain for reticulin, using acidified potassium permanganate, oxalic acid, iron alum, silver nitrate, formaldehyde, gold chloride, and sodium thiosulfate. (05 Mar 2000) |
| Gram's stain | <microbiology, technique> A method for differential staining of bacteria; smears are fixed by flaming, stained in a solution of crystal violet, treated with iodine solution, rinsed, decolorised, and then counterstained with safranin O. Gram-positive organisms stain purple black and Gram-negative organisms stain pink; useful in bacterial taxonomy and identification, and also in indicating fundamental differences in cell wall structure. (05 Mar 2000) |
| Gram stain | <microbiology, procedure> A method of staining bacteria using a violet stain. The gram staining characteristics (denoted as positive or negative) can assist in the identification of the offending bacteria. A heat fixed bacterial smear is stained with crystal violet (methyl violet), treated with 3% iodine/potassium iodide solution, washed with alcohol and counterstained. The method differentiates bacteria into two main classes, gram-positive and gram-negative. Certain bacteria, notably mycobacteria, that have walls with high lipid content show acid-fast staining the stain resists decolouration in strong acid. (27 Sep 1997) |
| periodic acid Schiff stain | <technique> A histochemical technique based on periodic acid oxidation of a substance containing the 1,2-glycol grouping. It is used for staining carbohydrates as the resulting dialdehyde reacts with Schiff reagent to form a coloured product. Substances that can be demonstrated include carbohydrates, mucins, cartilage matrix, collagen, reticulum, basement membranes, fibrin, thyroid colloid, amyloid, glomerular hyaline deposits, and a number of other secretions or tissue constituents. Also used in for staining gels on which glycoproteins have been run. See: periodic acid Schiff reaction Synonym: PAS stain. (22 Sep 2002) |
| green stain | <technique> A deposit, produced by chromogenic bacteria, found on the cervicolabial portions of the teeth, usually in children. See: acquired pellicle. (05 Mar 2000) |
| Perls' Prussian blue stain | <technique> A stain for ferric iron as in haemosiderins, using potassium ferrocyanide in acetic acid or dilute hydrochloric acid followed by a red counterstain such as safranin O or neutral red; various haemosiderins and most mineral irons give a blue-green reaction, while nuclei stain red. (05 Mar 2000) |
| Gridley's stain | <technique> For fungi, a method for fixed tissue sections based on Bauer's chromic acid leucofuchsin stain with the addition of Gomori's aldehyde fuchsin stain and metanil yellow as counterstains; against a yellow background, hyphae, conidia, yeast capsules, elastin, and mucin appear in different shades of blue to purple. (05 Mar 2000) |
| metachromatic stain | <technique> A stain, such as methylene blue, thionin, or azure A, that has the ability to produce different colours with various histological or cytological structures. (05 Mar 2000) |