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Heidenhain's iron haematoxylin stain <technique> An iron alum haematoxylin stain used for staining muscle striations and mitotic structures blue-black.
(05 Mar 2000)
Schaeffer-Fulton stain <technique> A stain for bacterial spores using malachite green and safranin so that bacterial bodies are red to pink and spores are green.
(05 Mar 2000)
Schmorl's ferric-ferricyanide reduction stain <technique> A stain to test for reducing substances in tissues, including melanin, argentaffin granules, thyroid colloid, keratin, keratohyalin, and lipofuscin pigments; ferricyanide is converted into ferrocyanide which is converted to insoluble Prussian blue in the presence of ferric ions.
(05 Mar 2000)
Schmorl's picrothionin stain <technique> A stain for compact bone which employs thionin and picric acid solutions to produce blue to blue-black staining of bone canaliculi and cells; bone matrix is yellowish and cartilage ground substance is purple.
(05 Mar 2000)
Schultz stain <technique> A stain for cholesterol; a relatively specific but insensitive histochemical test for cholesterol and cholesterol esters in which frozen sections of formalin-fixed tissues are oxidised in iron alum, hydrogen peroxide, or sodium iodate, then treated with sulfuric acid to give a blue-green to red colour in a positive reaction; the presence of glycerol inhibits the reaction.
(05 Mar 2000)
Prussian blue stain <technique> A stain employing acid potassium ferrocyanide to demonstrate iron, as in siderocytes.
(05 Mar 2000)
Hirsch-Peiffer stain <technique> A stain used for cytologic demonstration staining of metachromatic leukodystrophy; excess sulfatides stain metachromatically (golden brown) with cresyl violet in acetic acid.
(05 Mar 2000)
Hiss' stain <technique> A stain for demonstrating the capsules of microorganisms, using gentian violet or basic fuchsin followed by a copper sulphate wash.
(05 Mar 2000)
PTA stain <technique> The first general stain used for electron microscopy; a selective stain for extracellular components such as elastin, collagen, and basement membrane mucopolysaccharides; it can be followed by uranyl acetate or lead.
Synonym: PTA stain.
(05 Mar 2000)
Holmes' stain <technique> A silver nitrate staining method for nerve fibres.
(05 Mar 2000)
Puchtler-Sweat stain <technique> For basement membranes, a staining method using resorcin-fuchsin and nuclear fast red solutions after Carnoy's fixative; basement membranes are gray to black and nuclei pink to red.
For haemoglobin and haemosiderin, a complex staining method in which, on a yellow background, haemoglobin is stained red, haemosiderin blue to green and elastic fibres are pink.
(05 Mar 2000)
selective stain <technique> A stain that colours one portion of a tissue or cell exclusively or more deeply than the remaining portions.
(05 Mar 2000)
Hortega's neuroglia stain <technique> One of several silver carbonate methods to demonstrate astrocytes, oligodendroglia, and microglia.
(05 Mar 2000)
Hucker-Conn stain <technique> A crystal violet-ammonium oxalate mixture used in Gram's stain.
(05 Mar 2000)
silver-ammoniacal silver stain <technique> A stain for the acid protein component of nucleolar regions which are active or which were transcriptionally active in the preceding interphase; uses silver nitrate, ammoniacal silver, and formalin.
Synonym: Ag-AS stain.
(05 Mar 2000)
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