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Schmorl's ferric-ferricyanide reduction stain <technique> A stain to test for reducing substances in tissues, including melanin, argentaffin granules, thyroid colloid, keratin, keratohyalin, and lipofuscin pigments; ferricyanide is converted into ferrocyanide which is converted to insoluble Prussian blue in the presence of ferric ions.
(05 Mar 2000)
Schmorl's picrothionin stain <technique> A stain for compact bone which employs thionin and picric acid solutions to produce blue to blue-black staining of bone canaliculi and cells; bone matrix is yellowish and cartilage ground substance is purple.
(05 Mar 2000)
Schultz stain <technique> A stain for cholesterol; a relatively specific but insensitive histochemical test for cholesterol and cholesterol esters in which frozen sections of formalin-fixed tissues are oxidised in iron alum, hydrogen peroxide, or sodium iodate, then treated with sulfuric acid to give a blue-green to red colour in a positive reaction; the presence of glycerol inhibits the reaction.
(05 Mar 2000)
Prussian blue stain <technique> A stain employing acid potassium ferrocyanide to demonstrate iron, as in siderocytes.
(05 Mar 2000)
Hirsch-Peiffer stain <technique> A stain used for cytologic demonstration staining of metachromatic leukodystrophy; excess sulfatides stain metachromatically (golden brown) with cresyl violet in acetic acid.
(05 Mar 2000)
Hiss' stain <technique> A stain for demonstrating the capsules of microorganisms, using gentian violet or basic fuchsin followed by a copper sulphate wash.
(05 Mar 2000)
PTA stain <technique> The first general stain used for electron microscopy; a selective stain for extracellular components such as elastin, collagen, and basement membrane mucopolysaccharides; it can be followed by uranyl acetate or lead.
Synonym: PTA stain.
(05 Mar 2000)
Holmes' stain <technique> A silver nitrate staining method for nerve fibres.
(05 Mar 2000)
Puchtler-Sweat stain <technique> For basement membranes, a staining method using resorcin-fuchsin and nuclear fast red solutions after Carnoy's fixative; basement membranes are gray to black and nuclei pink to red.
For haemoglobin and haemosiderin, a complex staining method in which, on a yellow background, haemoglobin is stained red, haemosiderin blue to green and elastic fibres are pink.
(05 Mar 2000)
selective stain <technique> A stain that colours one portion of a tissue or cell exclusively or more deeply than the remaining portions.
(05 Mar 2000)
Hortega's neuroglia stain <technique> One of several silver carbonate methods to demonstrate astrocytes, oligodendroglia, and microglia.
(05 Mar 2000)
Hucker-Conn stain <technique> A crystal violet-ammonium oxalate mixture used in Gram's stain.
(05 Mar 2000)
silver-ammoniacal silver stain <technique> A stain for the acid protein component of nucleolar regions which are active or which were transcriptionally active in the preceding interphase; uses silver nitrate, ammoniacal silver, and formalin.
Synonym: Ag-AS stain.
(05 Mar 2000)
silver protein stain <technique> A silver proteinate complex used in staining nerve fibres, nerve endings, and flagellate protozoa; also used to demonstrate phagocytosis in living animals by the cells of the reticuloendothelial system.
(05 Mar 2000)
silver stain <technique> Any of a variety of stain's (e.g., Bielschowsky's, Gomori's silver, impregnation stain's) which employ alkaline silver nitrate solutions to stain connective tissue fibres (reticulin, collagen), calcium salt deposits, spirochaetes, neurological tissue, and nucleolar organiser regions.
(05 Mar 2000)
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