| Weigert's stain | <technique> 1. For actinomyces, a staining method using immersion in a dark red orsellin solution in alcohol, then staining in crystal-violet solution. See: iron haematoxylin. 2. For elastin, a staining solution of fuchsin, resorcin, and ferric chloride; elastic fibres stain blue-black. 3. For fibrin, a staining method using solutions of aniline-crystal violet and iodine-potassium iodide, then decolorizing in aniline oil and xylol; the fibrin is stained dark blue. 4. For myelin, a staining method using ferric chloride and haematoxylin; myelin stains deep blue, degenerated portions a light yellowish colour. 5. For neuroglia, a complicated process in which the final treatment is like that for staining fibrin; neuroglia and nuclei stain blue. 6. For reticulum, a silver impregnation technique in which reticulum appears as black, well-defined fibres without beading and with a relatively clear background. (05 Mar 2000) |
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| Mowry's colloidal iron stain | <technique> A stain used for demonstrating acid mucopolysaccharides. (05 Mar 2000) |
| Williams' stain | <technique> A stain for Negri bodies which utilises picric acid, fuchsin, and methylene blue; Negri bodies are magenta, granules and nerve cells blue, and erythrocytes yellowish. (05 Mar 2000) |
| plasma stain | <technique> Plasmatic stainplasmic stain, a stain whose principal affinity is for the cytoplasm of cells. (05 Mar 2000) |
| MSB trichrome stain | <technique> A stain for fibrin using martius yellow, brilliant crystal scarlet 6R, and soluble blue; fibrin is selectively stained red and connective tissue appears blue. (05 Mar 2000) |
| plastic section stain | <technique> For electron microscopy, a stain (e.g., osmic acid, PTA, potassium permanganate) used on thin sections of plastic-embedded tissues, utilizing differential attachment of heavy atoms to various cellular and tissue structures so that electrons will be absorbed and scattered by these structures to produce an image; to achieve differential staining, the stain must penetrate nonwettable plastic embedments, for light microscopy, a stain (e.g., alkaline toluidine blue, silver methenamine) used on plastic-embedded tissues to attain higher resolution and more detail than normally possible; semi-thick (0.5-1.5 um) sections are particularly useful in renal pathology, especially in combination with the phase microscope. (05 Mar 2000) |
| multiple stain | <technique> A mixture of several dyes each having an independent selective action on one or more portions of the tissue. (05 Mar 2000) |
| Wright's stain | <technique> A staining mixture of eosinates of polychromed methylene blue used in staining of blood smears. (05 Mar 2000) |
| contrast stain | <technique> A dye used to colour one portion of a tissue or cell which remained unaffected when the other part was stained by a dye of different colour. Synonym: differential stain. (05 Mar 2000) |
| port-wine stain | <technique> A mark on the skin that resembles port wine (porto) in its rich ruby red colour. Due to an abnormal aggregation of capillaries, a port-wine stain is a type of haemangioma. It occurs on the face as a sign of sturge-weber syndrome. (12 Dec 1998) |
| positive stain | <technique> Direct binding of a dye with a tissue component to produce contrast; in electron microscopy, heavy metals like uranyl and lead salts are used to bind to selective cell constituents to produce increased density to the electron beam, i.e., contrast. (05 Mar 2000) |
| haematoxylin and eosin stain | <technique> Probably the most generally useful of all staining methods for tissues; nuclei are stained a deep blue-black with haematoxylin, and cytoplasm is stained pink after counterstaining with eosin, usually in water. (05 Mar 2000) |
| haematoxylin-malachite green-basic fuchsin stain | <technique> A stain for epoxy resin-extracted sections; semi-thick sections have their plastic dissolved out and the residual tissue is stained sequentially with the various dyes; nuclei and astrocytes are purplish-pink and myelin, lipid droplets, nucleoli, and oligodendrocytes are bright blue-green. (05 Mar 2000) |
| haematoxylin-phloxine B stain | <technique> A stain for intact epoxy sections; semi-thick sections of plastic-embedded tissues have the following structures stained blue to black; chromatin, nucleoli, basophilic cytoplasm, mitochondria, plasma and nuclear membranes, anisotropic myofibrils, mast cell granules, and elastic membranes of blood vessels; appearing pink to red are collagen fibrils, reticulum, goblet cell mucins, hyalin cartilage matrix, stereocilia, cytoplasm, and erythrocytes; fat droplets and perichondrocyte matrix are green. (05 Mar 2000) |
| Hale's colloidal iron stain | <technique> A stain used to distinguish acid mucopolysaccharides such as hyaluronic acid; may be combined with PAS to also visualise carbohydrate-containing proteins and glycoproteins. (05 Mar 2000) |