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Roux's stain <technique> A double stain for diphtheria bacilli which employs crystal violet or dahlia and methyl green.
(05 Mar 2000)
Ciaccio's stain <technique> A method for demonstrating complex insoluble intra-cellular lipids using fixation in a formalin-dichromate solution, embedding in paraffin, staining with Sudan III or IV, and examination in aqueous mountant.
(05 Mar 2000)
phosphotungstic acid stain <technique> The first general stain used for electron microscopy; a selective stain for extracellular components such as elastin, collagen, and basement membrane mucopolysaccharides; it can be followed by uranyl acetate or lead.
Synonym: PTA stain.
(05 Mar 2000)
Wachstein-Meissel stain <technique> For calcium-magnesium-ATPase, a method similar to that of Gomori's non-specific acid phosphatase stain, except that incubation is carried out with ATP as substrate at neutral pH; enzyme activity is generally demonstrated at cell membranes.
(05 Mar 2000)
Warthin-Starry silver stain <technique> A stain for spirochetes in which preparations are incubated in 1% silver nitrate solution followed by a developer.
(05 Mar 2000)
picrocarmine stain <technique> A red crystalline powder derived from a solution of carmine, ammonia, and picric acid which is evaporated, leaving the powder (soluble in water); it produces excellent staining of keratohyaline granules.
(05 Mar 2000)
picro-Mallory trichrome stain <technique> A modification of Mallory's trichrome stain that involves the addition of picric acid.
(05 Mar 2000)
picronigrosin stain <technique> A solution of nigrosin in picric acid, used for staining connective tissue.
(05 Mar 2000)
Weigert-Gram stain <technique> A stain for bacteria in tissues in which sections are stained in alum-haematoxylin, then in eosin, aniline methyl violet, and Lugol's solution.
(05 Mar 2000)
Weigert's iron haematoxylin stain <technique> A nuclear staining solution containing haematoxylin, ferric chloride, and hydrochloric acid; useful in combination with von Gieson's stain, especially for demonstrating connective tissue elements or Entamoeba histolytica in sections.
(05 Mar 2000)
Weigert's stain <technique> 1. For actinomyces, a staining method using immersion in a dark red orsellin solution in alcohol, then staining in crystal-violet solution.
See: iron haematoxylin.
2. For elastin, a staining solution of fuchsin, resorcin, and ferric chloride; elastic fibres stain blue-black.
3. For fibrin, a staining method using solutions of aniline-crystal violet and iodine-potassium iodide, then decolorizing in aniline oil and xylol; the fibrin is stained dark blue.
4. For myelin, a staining method using ferric chloride and haematoxylin; myelin stains deep blue, degenerated portions a light yellowish colour.
5. For neuroglia, a complicated process in which the final treatment is like that for staining fibrin; neuroglia and nuclei stain blue.
6. For reticulum, a silver impregnation technique in which reticulum appears as black, well-defined fibres without beading and with a relatively clear background.
(05 Mar 2000)
Mowry's colloidal iron stain <technique> A stain used for demonstrating acid mucopolysaccharides.
(05 Mar 2000)
Williams' stain <technique> A stain for Negri bodies which utilises picric acid, fuchsin, and methylene blue; Negri bodies are magenta, granules and nerve cells blue, and erythrocytes yellowish.
(05 Mar 2000)
plasma stain <technique> Plasmatic stainplasmic stain, a stain whose principal affinity is for the cytoplasm of cells.
(05 Mar 2000)
MSB trichrome stain <technique> A stain for fibrin using martius yellow, brilliant crystal scarlet 6R, and soluble blue; fibrin is selectively stained red and connective tissue appears blue.
(05 Mar 2000)
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