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Mowry's colloidal iron stain <technique> A stain used for demonstrating acid mucopolysaccharides.
(05 Mar 2000)
Williams' stain <technique> A stain for Negri bodies which utilises picric acid, fuchsin, and methylene blue; Negri bodies are magenta, granules and nerve cells blue, and erythrocytes yellowish.
(05 Mar 2000)
plasma stain <technique> Plasmatic stainplasmic stain, a stain whose principal affinity is for the cytoplasm of cells.
(05 Mar 2000)
MSB trichrome stain <technique> A stain for fibrin using martius yellow, brilliant crystal scarlet 6R, and soluble blue; fibrin is selectively stained red and connective tissue appears blue.
(05 Mar 2000)
plastic section stain <technique> For electron microscopy, a stain (e.g., osmic acid, PTA, potassium permanganate) used on thin sections of plastic-embedded tissues, utilizing differential attachment of heavy atoms to various cellular and tissue structures so that electrons will be absorbed and scattered by these structures to produce an image; to achieve differential staining, the stain must penetrate nonwettable plastic embedments, for light microscopy, a stain (e.g., alkaline toluidine blue, silver methenamine) used on plastic-embedded tissues to attain higher resolution and more detail than normally possible; semi-thick (0.5-1.5 um) sections are particularly useful in renal pathology, especially in combination with the phase microscope.
(05 Mar 2000)
multiple stain <technique> A mixture of several dyes each having an independent selective action on one or more portions of the tissue.
(05 Mar 2000)
Wright's stain <technique> A staining mixture of eosinates of polychromed methylene blue used in staining of blood smears.
(05 Mar 2000)
contrast stain <technique> A dye used to colour one portion of a tissue or cell which remained unaffected when the other part was stained by a dye of different colour.
Synonym: differential stain.
(05 Mar 2000)
port-wine stain <technique> A mark on the skin that resembles port wine (porto) in its rich ruby red colour. Due to an abnormal aggregation of capillaries, a port-wine stain is a type of haemangioma. It occurs on the face as a sign of sturge-weber syndrome.
(12 Dec 1998)
positive stain <technique> Direct binding of a dye with a tissue component to produce contrast; in electron microscopy, heavy metals like uranyl and lead salts are used to bind to selective cell constituents to produce increased density to the electron beam, i.e., contrast.
(05 Mar 2000)
haematoxylin and eosin stain <technique> Probably the most generally useful of all staining methods for tissues; nuclei are stained a deep blue-black with haematoxylin, and cytoplasm is stained pink after counterstaining with eosin, usually in water.
(05 Mar 2000)
haematoxylin-malachite green-basic fuchsin stain <technique> A stain for epoxy resin-extracted sections; semi-thick sections have their plastic dissolved out and the residual tissue is stained sequentially with the various dyes; nuclei and astrocytes are purplish-pink and myelin, lipid droplets, nucleoli, and oligodendrocytes are bright blue-green.
(05 Mar 2000)
haematoxylin-phloxine B stain <technique> A stain for intact epoxy sections; semi-thick sections of plastic-embedded tissues have the following structures stained blue to black; chromatin, nucleoli, basophilic cytoplasm, mitochondria, plasma and nuclear membranes, anisotropic myofibrils, mast cell granules, and elastic membranes of blood vessels; appearing pink to red are collagen fibrils, reticulum, goblet cell mucins, hyalin cartilage matrix, stereocilia, cytoplasm, and erythrocytes; fat droplets and perichondrocyte matrix are green.
(05 Mar 2000)
Hale's colloidal iron stain <technique> A stain used to distinguish acid mucopolysaccharides such as hyaluronic acid; may be combined with PAS to also visualise carbohydrate-containing proteins and glycoproteins.
(05 Mar 2000)
half-a-gram stain <technique> A lab technique used to detect the presence of members from the bacterial family Legionellaceae in samples of sputum.
(09 Oct 1997)
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