| peroxidase stain | <technique> A method for demonstrating peroxidase granules in some neutrophils and in eosinophils; the enzyme promotes the oxidation of benzidine by hydrogen peroxide; tissues treated with horseradish peroxidase can also have the enzyme detected in the electron microscope. (05 Mar 2000) |
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| methyl green-pyronin stain | <technique> A staining method useful for identification of plasma cells which are intensely pyroninophilic; a mixture of a green and a red dye that has the property of staining highly polymerised nucleic acid (DNA) green and low molecular weight nucleic acids (RNA) red. See: Unna-Pappenheim stain. (05 Mar 2000) |
| Romanovsky type stain | <technique> Composite histological stains including methylene blue, Azure A or B and eosin, sometimes with other stains. Examples are Giemsa, Wright's and Leishman's stain. (18 Nov 1997) |
| Romanowsky's blood stain | <technique> Prototype of the eosin-methylene blue stain's for blood smears, using aqueous solutions made of a mixture of methylene blue (saturated) and eosin. Romanowsky-type stain's depend for their action on compounds formed by interaction of methylene blue and eosin; most are of no value if water is present in the alcohol because neutral dyes become precipitated. (05 Mar 2000) |
| chromate stain | <technique> For lead, a method in which tissues preserved in chromate-containing fixatives, such as Regaud's or Orth's fixatives, precipitate lead as yellow lead chromate crystals; formalin-fixed sections are treated with potassium chromate acidified with acetic acid. (05 Mar 2000) |
| chrome alum haematoxylin-phloxine stain | <technique> A stain used to demonstrate pancreatic islet cells; alpha cells appear red, beta cells blue or unstained. (05 Mar 2000) |
| Roux's stain | <technique> A double stain for diphtheria bacilli which employs crystal violet or dahlia and methyl green. (05 Mar 2000) |
| Ciaccio's stain | <technique> A method for demonstrating complex insoluble intra-cellular lipids using fixation in a formalin-dichromate solution, embedding in paraffin, staining with Sudan III or IV, and examination in aqueous mountant. (05 Mar 2000) |
| phosphotungstic acid stain | <technique> The first general stain used for electron microscopy; a selective stain for extracellular components such as elastin, collagen, and basement membrane mucopolysaccharides; it can be followed by uranyl acetate or lead. Synonym: PTA stain. (05 Mar 2000) |
| Wachstein-Meissel stain | <technique> For calcium-magnesium-ATPase, a method similar to that of Gomori's non-specific acid phosphatase stain, except that incubation is carried out with ATP as substrate at neutral pH; enzyme activity is generally demonstrated at cell membranes. (05 Mar 2000) |
| Warthin-Starry silver stain | <technique> A stain for spirochetes in which preparations are incubated in 1% silver nitrate solution followed by a developer. (05 Mar 2000) |
| picrocarmine stain | <technique> A red crystalline powder derived from a solution of carmine, ammonia, and picric acid which is evaporated, leaving the powder (soluble in water); it produces excellent staining of keratohyaline granules. (05 Mar 2000) |
| picro-Mallory trichrome stain | <technique> A modification of Mallory's trichrome stain that involves the addition of picric acid. (05 Mar 2000) |
| picronigrosin stain | <technique> A solution of nigrosin in picric acid, used for staining connective tissue. (05 Mar 2000) |
| Weigert-Gram stain | <technique> A stain for bacteria in tissues in which sections are stained in alum-haematoxylin, then in eosin, aniline methyl violet, and Lugol's solution. (05 Mar 2000) |