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glucose oxidase [EC 1.1.3.4] an enzyme of the oxidoreductase class that catalyzes the oxidation of glucose, reducing oxygen to hydrogen peroxide. It is a flavoprotein, containing FAD, and is highly specific for β-D-glucose. The enzyme is produced by Penicillium notatum and other fungi and has antibacterial activity in the presence of glucose and oxygen because of the hydrogen peroxide produced. It is used to estimate glucose concentration in blood or urine samples through the formation of colored dyes by the hydrogen peroxide.
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glucose t. any of various laboratory tests for glucose in the urine; many formerly common ones are no longer used. See Benedict's t., Rubner's t., and saccharimeter t.
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glucose t., impaired a term denoting values of fasting plasma glucose or results of an oral glucose tolerance test that are abnormal but not high enough to be diagnostic of diabetes mellitus. Formerly called chemical, latent, preclinical, or subclinical diabetes.
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glucose tolerance f. a biologically active complex of chromium and nicotinic acid that facilitates the reaction of insulin with receptor sites on tissues.
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glucose tolerance t. a metabolic test of carbohydrate tolerance, measuring active insulin, a hepatic function based on the power of the normal liver to absorb and store large quantities of glucose, and the effectiveness of intestinal absorption of glucose. The most common method is the oral glucose tolerance test (q.v.).
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