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fluorescent antibody technique, indirect A form of fluorescent antibody technique commonly used to detect serum antibodies and immune complexes in tissues and microorganisms in specimens from patients with infectious diseases. The technique involves formation of an antigen-antibody complex which is labelled with fluorescein-conjugated anti-immunoglobulin antibody.
(12 Dec 1998)
fluorescent antibody techniques Lab techniques for locating antigens in a prepared tissue sample by using antibodies with fluorescent labels which will bind to the antigens of interest.
(09 Oct 1997)
fluorescent antibody titre An indirect method of measuring the concentration of an antibody in serum through the detection of fluorescent light emitted in a chemical reaction.
(27 Sep 1997)
fluorescent antinuclear antibody test FANA test, a test for antinuclear antibody components; used, in particular, for the diagnosis of collagen-vascular diseases.
(05 Mar 2000)
fluorescent dyes Dyes that emit light when exposed to light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags. They are used as markers in biochemistry and immunology.
(12 Dec 1998)
fluorescent in situ hybridization See FISH.
(12 Dec 1998)
fluorescent label A molecule which fluoresces and can be attached to a probe molecule that does not fluoresce.
(09 Oct 1997)
fluorescent microscope <instrument> A microscope equipped to examine material that fluoresces under ultraviolet (UV) light.
(12 Dec 1998)
fluorescent screen A screen coated with fluorescent crystals such as the calcium tungstate used in the fluoroscope.
(05 Mar 2000)
fluorescent stain <technique> A stain or staining procedure using a fluorescent dye or substance that will combine selectively with certain tissue components and that will then fluoresce upon irradiation with ultraviolet or violet-blue light.
(05 Mar 2000)
fluorescent treponemal antibody-absorption test <investigation> A sensitive and specific serologic test for syphilis. It is more specific than the VDRL assay.
The patient's serum is diluted to remove non-specific antibodies and then mixed on a glass slide with Nichol's strain of Treponema pallidum. If antibodies are present in the patient's serum they bind to the antigen and the bound antibodies are detected with fluoresceinated antihuman gamma-globulin antibody.
Synonym: FTA-ABS test.
(12 Sep2002)
fluorescin <chemistry> A colourless, amorphous substance which is produced by the reduction of fluorescein, and from which the latter may be formed by oxidation.
Source: Websters Dictionary
(01 Mar 1998)
fluoric <chemistry> Pertaining to, obtained from, or containing, fluorine.
Origin: Cf. F. Fluorique.
Source: Websters Dictionary
(01 Mar 1998)
fluoridated teeth <dentistry> Teeth exposed to fluorine salts during odontogenesis.
(05 Mar 2000)
fluoridation Addition of fluorides to a community water supply, usually 1 ppm, to reduce incidence of dental decay.
(05 Mar 2000)
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