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Schmidt-Thannhauser method A method for fractionation of nucleic acid, based upon the fact that RNA but not DNA is hydrolyzed to nucleotides by alkali; RNA can be hydrolyzed in about 2 hours in 0.75 n NaOH, but 18 hours and 0.3 n NaOH usually are used.
(05 Mar 2000)
Schweninger's method A method suggested to reduce obesity by restricting intake of fluid.
(05 Mar 2000)
scientific method The universally-accepted, organised approach to the study of science, which consists of the following steps:
1. Observation - collecting data.
2. Hypothesis - forming a preliminary possible explanation of the data.
3. Testing - test the hypothesis by collecting more data.
4. Results - interpreting the results of the test and deciding if the hypothesis should be rejected. The hypothesis is rejected if the results contradict it, showing that it is wrong.
5. Conclusion - stating a conclusion that can be evaluated independently by others.
(09 Oct 1997)
hexokinase method The most specific method for measuring glucose in serum or plasma, wherein hexokinase plus ATP transforms glucose to glucose-6-phosphate plus ADP; glucose-6-phosphate is then reacted with NADP and glucose-6-phosphate dehydrogenase to form NADP which is measured spectrophotometrically.
(05 Mar 2000)
Hilton's method Division of the nerves supplying a part, for the relief of pain in ulcers.
(05 Mar 2000)
Hirschberg's method A method of measuring the amount of deviation of a strabismic eye, by observing the reflection of a light fixated by the straight eye on the cornea of the deviating eye.
(05 Mar 2000)
Purmann's method Treatment of aneurysm by extirpation of the sac.
(05 Mar 2000)
Hung's method A simple saline flotation method for concentrating helminth eggs in the faeces.
See: flotation method.
Synonym: Hung's method.
(05 Mar 2000)
Shaffer-Hartmann method An obsolete method for the quantitative determination of glucose in biological fluids, based on the reduction of copper by the reducing group of the sugar.
(05 Mar 2000)
shotgun method Cloning of DNA fragments randomly generated from a genome.
See: genomic library.
(09 Oct 1997)
single-blind method A method in which either the observer(s) or the subject(s) is kept ignorant of the group to which the subjects are assigned.
(12 Dec 1998)
Somogyi method A measure of the level of activity of amylase in blood serum, as analyzed by means of the Somogyi method (the most frequently used procedure); one unit is equivalent to 1 mg of reducing sugar liberated as glucose per 100 ml of serum, when an aliquot of the latter is mixed with a standard starch substrate (plus sodium chloride for maximal activation) and incubated for a standard time; normal range is 80 to 150 unit's, but values are usually not regarded as clinically significant unless they are greater than 200.
(05 Mar 2000)
split cast method A procedure for placing indexed casts on an articulator to facilitate their removal and replacement on the instrument, the procedure of checking the ability of an articulator to receive or be adjusted to a maxillomandibular relation record.
Synonym: Needles' split cast method.
(05 Mar 2000)
Stas-Otto method A method of extraction of alkaloids from plants and animal bodies: the substance is digested in alcohol and tartaric acid, the fatty and resinous matters are precipitated with water, the fluid is made alkaline, and the alkaloids are extracted with ether or chloroform.
(05 Mar 2000)
Needles' split cast method A procedure for placing indexed casts on an articulator to facilitate their removal and replacement on the instrument, the procedure of checking the ability of an articulator to receive or be adjusted to a maxillomandibular relation record.
Synonym: Needles' split cast method.
(05 Mar 2000)
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